Creation of new ICSI:More natural gamete fusion avoiding injection
| Project/Area Number |
15K15593
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Akita University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
熊谷 仁 秋田大学, 医学(系)研究科(研究院), 准教授 (60333936)
佐藤 亘 秋田大学, 医学部, 助教 (10726441)
椛嶋 克哉 秋田大学, 医学部, 技術系補佐員 (30615422)
佐藤 恵美子 秋田大学, 医学(系)研究科(研究院), 技術系補佐員 (00638273)
白澤 弘光 秋田大学, 医学(系)研究科(研究院), 助教 (60598019)
|
|---|
| Research Collaborator |
HASEGAWA Hisataka 神奈川レディースクリニック, 胚培養士
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
|---|
| Keywords | 顕微授精 / センダイウイルス / 膜融合 / センダイウィルス |
|---|
| Outline of Final Research Achievements |
In this study, for the purpose of establishing a novel micro-insemination method without the egg cell membrane penetration, fertilization experiments were conducted by membrane fusion of sperm-eggs using inactivated Sendai virus using mouse as a model.
First, in order to remove the outer acrosomal membrane of the spermatozoa, artificial acrosome reaction induction experiments using Ca ionophore A 23187 were performed. Progression of the acrosome reaction and exposure of the inner acrosomal membrane occurred due to elevation of calcium concentration in spermatozoa, but as a result of carrying out fusion experiment using Sendai virus after the acrosome reaction induction treatment, finally sperm-egg membrane It did not lead to fusion.
|
Report
(4 results)
Research Products
(18 results)
-
[Journal Article] The location of “8”-shaped hatching influences inner cell mass formation in mouse blastocysts.2017
Author(s)
Yohei Onodera, Kazumasa Takahashi, Mayumi Goto, Mibuki Anzai, Natsuki Ono, Hiromitsu Shirasawa, Wataru Sato, Hiroshi Miura, Naoki Sato, Akira Sato, Yukiyo Kumazawa, Yukihiro Terada
-
Journal Title
PLoS ONE
Volume: 12
Issue: 4
Pages: e0175150-e0175150
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Time-lapse monitoring reveals that vitrification increases the frequency of contraction during the pre-hatching stage in mouse embryos2016
Author(s)
Shimoda Y,Kumagai J, Anzai M,Kabashima K,Togashi K,Miura Y,Shirasawa H,Sato W,Kumazawa Y, Terada Y
-
Journal Title
Journal of Reproduction and Development
Volume: 62
Issue: 2
Pages: 187-193
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Dysfunction in gap junction intercellular communication induces aberrant behavior of the inner cell mass and frequent collapses of expanded blastocysts in mouse embyos.2015
Author(s)
Togashi K, Kumagai J, Sato E, Shirasawa H, Shimoda Y, Makino K, Sato W, Kumazawa Y, Omori Y, Terada, Y.
-
Journal Title
J. Assist. Reprod. Genet.
Volume: 32
Issue: 6
Pages: 969-976
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets.2015
Author(s)
Shirasawa H,Kumagai J, Sato E, Kabashima K,Kumazawa Y,Sato W,Miura H,Nakamura R,Nanjo H, Minamiya Y,Akagami Y,Terada Y
-
Journal Title
Scientific Reports
Volume: 5
Issue: 1
Pages: 15371-15371
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
