| Project/Area Number |
15K15593
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Akita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
熊谷 仁 秋田大学, 医学(系)研究科(研究院), 准教授 (60333936)
佐藤 亘 秋田大学, 医学部, 助教 (10726441)
椛嶋 克哉 秋田大学, 医学部, 技術系補佐員 (30615422)
佐藤 恵美子 秋田大学, 医学(系)研究科(研究院), 技術系補佐員 (00638273)
白澤 弘光 秋田大学, 医学(系)研究科(研究院), 助教 (60598019)
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| Research Collaborator |
HASEGAWA Hisataka 神奈川レディースクリニック, 胚培養士
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 顕微授精 / センダイウイルス / 膜融合 / センダイウィルス |
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| Outline of Final Research Achievements |
In this study, for the purpose of establishing a novel micro-insemination method without the egg cell membrane penetration, fertilization experiments were conducted by membrane fusion of sperm-eggs using inactivated Sendai virus using mouse as a model.
First, in order to remove the outer acrosomal membrane of the spermatozoa, artificial acrosome reaction induction experiments using Ca ionophore A 23187 were performed. Progression of the acrosome reaction and exposure of the inner acrosomal membrane occurred due to elevation of calcium concentration in spermatozoa, but as a result of carrying out fusion experiment using Sendai virus after the acrosome reaction induction treatment, finally sperm-egg membrane It did not lead to fusion.
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