Getting genetic information from IVF media for noninvasive prenatal genetic screening
| Project/Area Number |
15K15594
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Chiba University |
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Principal Investigator |
SHOZU Makio 千葉大学, 大学院医学研究院, 教授 (30226302)
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| Research Collaborator |
KOBAYASHI Tatsuya
SONEHARA Hiroki
TERAMOTO Syokichi
SEGAWA Tomoya
OOKUBO Tsuyoshi
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | 産婦人科学 / 産科学 / 出生前診断 / 体外受精胚移殖 / 流産 / 体外受精胚移植 |
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| Outline of Final Research Achievements |
We examined what kind of genetic information are achievable from blastocyst culture media for possible non-invasive prenatal genetic screening. We found that serum hCG level within one week after implantation was inversely correlated with the number of X-chromosomes. We measured hCG level in used blastocyst culture medium and found that extremely high as well as low levels often resulted in non-implantation or abortion. Trace amount of genomic DNA was detected in the used medium, while mRNA was not. Significant amount of miRNA such as miR-22 as well as mitochondrial DNA (D-loop) were detectable in the blastocyst culture medium. Further study is warranted to determining relationship between these information and genetic constitution of embryo.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Ovarian nongestational choriocarcinoma and associated adenocarcinoma with the same germ cell origin determined by a molecular genetic approach: A case report.2016
Author(s)
Koyanagi T, Fujiwara H, Usui H, Ariga H, Machida S, Takei Y, Saga Y, Shozu M, Fukushima N, Niki T, Matsubara S, Suzuki M.
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Journal Title
Pathol Int.
Volume: 66
Issue: 9
Pages: 529-534
DOI
Related Report
Peer Reviewed
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