Identification and functional analysis of Progesterone target gene in preterm birth
Project/Area Number |
15K15597
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Obstetrics and gynecology
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Research Institution | The University of Tokyo |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
HIROTA Yasushi 東京大学, 医学部附属病院, 講師 (40598653)
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Project Period (FY) |
2015-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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Keywords | 早産 / プロゲステロン / プロゲステロン受容体 / 脱落膜 / 子宮 / 標的遺伝子 / 黄体ホルモン / RNA-seq / ChIP-seq |
Outline of Final Research Achievements |
Progesterone (P4) acts via the Progesterone receptor (PR). In pregnant women, P4 increases throughout pregnancy and remains high during labor and delivery. Given that levels of P4 do not decline before parturition, human parturition is involved in a functional PR withdrawal. It is speculated that preterm birth associated with the early decline of functional PR response, in the preterm uterus, the expression of PR target genes are decreased compared with normal delivery. To find the PR target genes related with preterm birth, we experimented as follows; (1) Using Ishikawa cells derived from human endometrium, Fifteen genes were identified as P4 responsive genes. Out of these 15 genes, we proposed that TRIM22 is a direct target gene of PR. (2) We cultured the decidual cells from normal and preterm delivery, the gene expression profile to response of P4 was analyzed by RNA-sequencing. As a result, the 385 genes were the specific genes in the decidua cells of preterm delivery.
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Report
(2 results)
Research Products
(22 results)
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[Journal Article] Effects of 1,25-dihydroxy vitamin D3 on endometriosis.2016
Author(s)
Miyashita M, Koga K, Izumi G, Sue F, Makabe T, Taguchi A, Nagai M, Urata Y, Takamura M, Harada M, Hirata T, Hirota Y, Wada-Hiraike O, Fujii T, Osuga Y.
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Journal Title
J Clin Endocrinol Metab
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] A potential role of endoplasmic reticulum stress in development of ovarian hyperstimulation syndrome.2016
Author(s)
Takahashi N, Harada M, Hirota Y, Zhao L, Yoshino O, Urata Y, Izumi G, Takamura M, Hirata T, Koga K, Wada-Hiraike O, Fujii T, Osuga Y.
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Journal Title
Mol Cell Endocrinol
Volume: 428
Pages: 161-169
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Resveratrol Enhances Apoptosis in Endometriotic Stromal Cells.2016
Author(s)
Taguchi, A., Koga, K., Kawana, K., Makabe, T., Sue, F., Miyashita, M., Yoshida, M., Urata, Y., Izumi, G., Takamura, M., Harada, M., Hirata, T., Hirota, Y., Wada-Hiraike, O., Fujii, T., Osuga, Y
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Journal Title
American Journal of Reproductive Immunology
Volume: 75
Issue: 4
Pages: 486-492
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Simultaneous Detection and Evaluation of Four Subsets of CD4+ T Lymphocyte in Lesions and Peripheral Blood in Endometriosis.2015
Author(s)
Takamura, M., Koga, K., Izumi, G., Hirata, T., Harada, M., Hirota, Y., Hiraike, O., Fujii, T., Osuga, Y
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Journal Title
American Journal of Reproductive Immunology
Volume: 74
Issue: 6
Pages: 480-486
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Evidence of the activation of unfolded protein response in granulosa and cumulus cells during follicular growth and maturation.2015
Author(s)
Harada, M., Nose, E., Takahashi, N., Hirota, Y., Hirata, T., Yoshino, O., Koga, K., Fujii, T., Osuga, Y
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Journal Title
Gynecological Endocrinology
Volume: 31
Issue: 10
Pages: 783-787
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Muscle Segment Homeobox Genes Direct Embryonic Diapause by Limiting Inflammation in the Uterus.2015
Author(s)
Cha, J., Burnum-Johnson, K.E., Bartos, A., Li, Y., Baker, ES., Tilton, S.C., Webb-Robertson, B.J., Piehowski, P.D., Monroe, M.E., Jegga, A.G., Murata, S., Hirota, Y., Dey, S.K.
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Journal Title
The Journal of Biological Chemistry
Volume: 290
Issue: 24
Pages: 15337-15349
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] MicroRNA-200a impairs progesterone signaling in the cervix, disturbing embryo implantation.2016
Author(s)
Yasushi Hirota, Hirofumi Haraguchi, Tomoko Saito-Fujita, Mahiro Egashira, Leona Matsumoto, Mitsunori Matsuo, Takehiro Hiraoka, Sudhansu K. Dey, Yutaka Osuga, Tomoyuki Fujii.
Organizer
International Symposium on Epigenome Dynamics and Regulation in Germ Cells
Place of Presentation
京都大学百周年時計台記念館(京都府京都市)
Year and Date
2016-02-18
Related Report
Int'l Joint Research
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[Presentation] Endometrial regeneration in the mouse model of decellularized matrix transplantation.2015
Author(s)
Takehiro Hiraoka, Yasushi Hirota, Tomoko Saito-Fujita, Tomoki Tanaka, Mitsunori Matsuo, Mahiro Egashira, Leona Matsumoto, Hirofumi Haraguchi, Katsuko S. Furukawa, Yutaka Osuga, Tomoyuki Fujii.
Organizer
第38回日本分子生物学会年会 (BMB 2015)
Place of Presentation
神戸ポートアイランド(兵庫県神戸市)
Year and Date
2015-12-02
Related Report
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[Presentation] Crucial role of hypoxia inducible factor 2α in the pregnant uterus.2015
Author(s)
Leona Matsumoto, Yasushi Hirota, Tomoko Saito-Fujita, Hirofumi Haraguchi, Mahiro Egashira, Mitsunori Matsuo, Takehiro Hiraoka, Kaori Koga, Sudhansu K. Dey, Tomoyuki Fujii, Yutaka Osuga.
Organizer
31st ESHRE Annual Meeting (ESHRE 2015)
Place of Presentation
Lisbon, Portugal
Year and Date
2015-06-15
Related Report
Int'l Joint Research
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[Presentation] MicroRNA controls receptivity to the embryo by regulating progesterone signaling in the cervix and uterus.2015
Author(s)
Hirofumi Haraguchi, Yasushi Hirota, Tomoko Saito-Fujita, Mahiro Egashira, Leona Matsumoto, Mitsunori Matsuo, Takehiro Hiraoka, Kaori Koga, Sudhansu K. Dey, Yutaka Osuga, Tomoyuki Fujii.
Organizer
IFFS/JSRM International Meeting 2015
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Year and Date
2015-04-26
Related Report
Int'l Joint Research
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