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Identification and functional analysis of Progesterone target gene in preterm birth

Research Project

Project/Area Number 15K15597
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Obstetrics and gynecology
Research InstitutionThe University of Tokyo

Principal Investigator

Kanatani Mayuko  東京大学, 医学部附属病院, 助教 (60748862)

Co-Investigator(Kenkyū-buntansha) HIROTA Yasushi  東京大学, 医学部附属病院, 講師 (40598653)
Project Period (FY) 2015-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords早産 / プロゲステロン / プロゲステロン受容体 / 脱落膜 / 子宮 / 標的遺伝子 / 黄体ホルモン / RNA-seq / ChIP-seq
Outline of Final Research Achievements

Progesterone (P4) acts via the Progesterone receptor (PR). In pregnant women, P4 increases throughout pregnancy and remains high during labor and delivery. Given that levels of P4 do not decline before parturition, human parturition is involved in a functional PR withdrawal. It is speculated that preterm birth associated with the early decline of functional PR response, in the preterm uterus, the expression of PR target genes are decreased compared with normal delivery. To find the PR target genes related with preterm birth, we experimented as follows; (1) Using Ishikawa cells derived from human endometrium, Fifteen genes were identified as P4 responsive genes. Out of these 15 genes, we proposed that TRIM22 is a direct target gene of PR. (2) We cultured the decidual cells from normal and preterm delivery, the gene expression profile to response of P4 was analyzed by RNA-sequencing. As a result, the 385 genes were the specific genes in the decidua cells of preterm delivery.

Report

(2 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (22 results)

All 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (14 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 9 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (6 results) (of which Int'l Joint Research: 5 results) Book (1 results)

  • [Int'l Joint Research] シンシナティ小児病院(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Effects of 1,25-dihydroxy vitamin D3 on endometriosis.2016

    • Author(s)
      Miyashita M, Koga K, Izumi G, Sue F, Makabe T, Taguchi A, Nagai M, Urata Y, Takamura M, Harada M, Hirata T, Hirota Y, Wada-Hiraike O, Fujii T, Osuga Y.
    • Journal Title

      J Clin Endocrinol Metab

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A potential role of endoplasmic reticulum stress in development of ovarian hyperstimulation syndrome.2016

    • Author(s)
      Takahashi N, Harada M, Hirota Y, Zhao L, Yoshino O, Urata Y, Izumi G, Takamura M, Hirata T, Koga K, Wada-Hiraike O, Fujii T, Osuga Y.
    • Journal Title

      Mol Cell Endocrinol

      Volume: 428 Pages: 161-169

    • DOI

      10.1016/j.mce.2016.03.032

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Resveratrol Enhances Apoptosis in Endometriotic Stromal Cells.2016

    • Author(s)
      Taguchi, A., Koga, K., Kawana, K., Makabe, T., Sue, F., Miyashita, M., Yoshida, M., Urata, Y., Izumi, G., Takamura, M., Harada, M., Hirata, T., Hirota, Y., Wada-Hiraike, O., Fujii, T., Osuga, Y
    • Journal Title

      American Journal of Reproductive Immunology

      Volume: 75 Issue: 4 Pages: 486-492

    • DOI

      10.1111/aji.12489

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of TRIM22 as a progesterone-responsive gene in Ishikawa endometrial cancer cells.2015

    • Author(s)
      Saito-Kanatani M, Urano T, Hiroi H, Momoeda M, Ito M, Fujii T, Inoue S
    • Journal Title

      J Steroid Biochem Mol Biol

      Volume: 154 Pages: 217-225

    • DOI

      10.1016/j.jsbmb.2015.08.024

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous Detection and Evaluation of Four Subsets of CD4+ T Lymphocyte in Lesions and Peripheral Blood in Endometriosis.2015

    • Author(s)
      Takamura, M., Koga, K., Izumi, G., Hirata, T., Harada, M., Hirota, Y., Hiraike, O., Fujii, T., Osuga, Y
    • Journal Title

      American Journal of Reproductive Immunology

      Volume: 74 Issue: 6 Pages: 480-486

    • DOI

      10.1111/aji.12426

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evidence of the activation of unfolded protein response in granulosa and cumulus cells during follicular growth and maturation.2015

    • Author(s)
      Harada, M., Nose, E., Takahashi, N., Hirota, Y., Hirata, T., Yoshino, O., Koga, K., Fujii, T., Osuga, Y
    • Journal Title

      Gynecological Endocrinology

      Volume: 31 Issue: 10 Pages: 783-787

    • DOI

      10.3109/09513590.2015.1062862

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Muscle Segment Homeobox Genes Direct Embryonic Diapause by Limiting Inflammation in the Uterus.2015

    • Author(s)
      Cha, J., Burnum-Johnson, K.E., Bartos, A., Li, Y., Baker, ES., Tilton, S.C., Webb-Robertson, B.J., Piehowski, P.D., Monroe, M.E., Jegga, A.G., Murata, S., Hirota, Y., Dey, S.K.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 290 Issue: 24 Pages: 15337-15349

    • DOI

      10.1074/jbc.m115.655001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] プロテオミクスを用いた妊娠成立・維持機構の研究.2015

    • Author(s)
      松尾光徳, 廣田泰.
    • Journal Title

      HORMONE FRONTIER IN GYNECOLOGY

      Volume: 22 Pages: 11-17

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Cyclic Stretch Augments Production of Neutrophil Chemokines, Matrix Metalloproteinases, and Activin A in Human Endometrial Stromal Cells.2015

    • Author(s)
      Izumi G, Koga K, Takamura M, Osuga Y.
    • Journal Title

      Am J Reprod Immunol

      Volume: epub Issue: 6 Pages: 501-506

    • DOI

      10.1111/aji.12359

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] How does Progesterone Support Embryo Implantation?2015

    • Author(s)
      Hiraoka T, Saito-Fujita T, Hirota Y.
    • Journal Title

      J Mamm Ova Res

      Volume: 32 Issue: 3 Pages: 87-94

    • DOI

      10.1274/jmor.32.87

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 急速に増大した仙尾部未熟奇形腫のため妊娠22週で子宮内胎児死亡となった1例2015

    • Author(s)
      矢野倫子、田口歩、齋藤真由子、中澤直子
    • Journal Title

      東京産科婦人科学会会誌

      Volume: 64 Pages: 30-33

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 着床における黄体ホルモンの作用2015

    • Author(s)
      平岡毅大、廣田泰
    • Journal Title

      産科と婦人科

      Volume: 82 Pages: 740-745

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 子宮内膜における着床制御因子 プロゲステロン・シグナルを中心に2015

    • Author(s)
      藤田知子、廣田泰
    • Journal Title

      HORMONE FRONTIER IN GYNECOLOGY

      Volume: 22 Pages: 176-179

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 種差からみた着床 ヒトとの共通点と相違点2015

    • Author(s)
      江頭真宏、廣田泰
    • Journal Title

      HORMONE FRONTIER IN GYNECOLOGY

      Volume: 22 Pages: 183-189

    • Related Report
      2015 Annual Research Report
  • [Presentation] MicroRNA-200a impairs progesterone signaling in the cervix, disturbing embryo implantation.2016

    • Author(s)
      Yasushi Hirota, Hirofumi Haraguchi, Tomoko Saito-Fujita, Mahiro Egashira, Leona Matsumoto, Mitsunori Matsuo, Takehiro Hiraoka, Sudhansu K. Dey, Yutaka Osuga, Tomoyuki Fujii.
    • Organizer
      International Symposium on Epigenome Dynamics and Regulation in Germ Cells
    • Place of Presentation
      京都大学百周年時計台記念館(京都府京都市)
    • Year and Date
      2016-02-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Endometrial regeneration in the mouse model of decellularized matrix transplantation.2016

    • Author(s)
      Takehiro Hiraoka, Yasushi Hirota.
    • Organizer
      International Symposium on Epigenome Dynamics and Regulation in Germ Cells
    • Place of Presentation
      京都大学百周年時計台記念館(京都府京都市)
    • Year and Date
      2016-02-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Endometrial regeneration in the mouse model of decellularized matrix transplantation.2015

    • Author(s)
      Takehiro Hiraoka, Yasushi Hirota, Tomoko Saito-Fujita, Tomoki Tanaka, Mitsunori Matsuo, Mahiro Egashira, Leona Matsumoto, Hirofumi Haraguchi, Katsuko S. Furukawa, Yutaka Osuga, Tomoyuki Fujii.
    • Organizer
      第38回日本分子生物学会年会 (BMB 2015)
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] Crucial role of hypoxia inducible factor 2α in the pregnant uterus.2015

    • Author(s)
      Leona Matsumoto, Yasushi Hirota, Tomoko Saito-Fujita, Hirofumi Haraguchi, Mahiro Egashira, Mitsunori Matsuo, Takehiro Hiraoka, Kaori Koga, Sudhansu K. Dey, Tomoyuki Fujii, Yutaka Osuga.
    • Organizer
      31st ESHRE Annual Meeting (ESHRE 2015)
    • Place of Presentation
      Lisbon, Portugal
    • Year and Date
      2015-06-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The presence of cellular senescence and its possible roles in the postpartum uterus.2015

    • Author(s)
      Mahiro Egashira, Yasushi Hirota, Tomoko Saito-Fujita, Hirofumi Haraguchi, Leona Matsumoto, Mitsunori Matsuo, Takehiro Hiraoka, Yutaka Osuga, Tomoyuki Fujii.
    • Organizer
      IFFS/JSRM International Meeting 2015
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2015-04-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] MicroRNA controls receptivity to the embryo by regulating progesterone signaling in the cervix and uterus.2015

    • Author(s)
      Hirofumi Haraguchi, Yasushi Hirota, Tomoko Saito-Fujita, Mahiro Egashira, Leona Matsumoto, Mitsunori Matsuo, Takehiro Hiraoka, Kaori Koga, Sudhansu K. Dey, Yutaka Osuga, Tomoyuki Fujii.
    • Organizer
      IFFS/JSRM International Meeting 2015
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2015-04-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Book] Uterine Endometrial Function2016

    • Author(s)
      Yasushi Hirota
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2022-01-27  

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