Elucidating the mechanism of resistance to chemotherapy in gynecologic cancer based on intratumor heterogeneity
Project/Area Number |
15K15598
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Obstetrics and gynecology
|
Research Institution | Niigata University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
吉原 弘祐 新潟大学, 医歯学系, 助教 (40547535)
加嶋 克則 新潟大学, 医歯学総合病院, 特任教授 (50345500)
関根 正幸 新潟大学, 医歯学系, 准教授 (70345502)
石黒 竜也 新潟大学, 医歯学総合病院, 助教 (80625690)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | intratumor heterogeneity / cancer genome / spheroid / Intratumor heterogeneity |
Outline of Final Research Achievements |
In this study, we generated total 11 spheroid cultures derived from gynecologic cancers. Before genomic analyses for spheroid cultures, we focused on Sex-determining region Y-box 2 (SOX2), which is an essential factor involved in the self-renewal and pluripotency of embryonic stem cells, to clarify the significance of SOX2 expression in endometrial cancer. SOX2 overexpression regulated the expression of cyclin-dependent kinase inhibitor 1A (CDKN1A), and SOX2 specifically bound to p21 promoter DNA in endometrial cancer cell lines expressing SOX2. Expressions of SOX2 in endometrial cancer patients were significantly correlated with histological grade and poor prognosis. Moreover, low p21 together with high SOX2 expressions in advanced endometrial cancer patients were associated with the most unfavorable outcomes of patients. These results indicated that simultaneous measurement of SOX2 and p21 expression in endometrial cancer patients may be a useful biomarker for patient prognosis.
|
Report
(3 results)
Research Products
(22 results)
-
[Journal Article] Establishment and characterization of an in vitro model of ovarian cancer stem-like cells with an enhanced proliferative capacity.2016
Author(s)
Ishiguro T, Sato A, Ohata H, Ikarashi Y, Takahashi R, Ochiya T, Yoshida M, Tsuda H, Onda T, Kato T, Kasamatsu T, Enomoto T, Tanaka K, Nakagama H, Okamoto K.
-
Journal Title
Cancer Research
Volume: 76
Issue: 1
Pages: 150-160
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
-
[Journal Article] The randomized phase 3 trial of irinotecan plus cisplatin compared with paclitaxel plus carboplatin as first line chemotherapy for ovarian clear cell carcinoma: JGOG3017/GCIG Trial.2016
Author(s)
Sugiyama T, Okamoto A, Enomoto T, Hamano T, Aotani E, Terao Y, Suzuki N, Mikami M, Yaegashi N, Kato K, Yoshikawa H, Yokoyama Y, Tanabe H, Nishino K, Nomura H, Kim JW, Kim BG, Pignata S, Alexandre J, Green J, Isonishi S, Terauchi F, Fujiwara K, Aoki D.
-
Journal Title
J Clin Oncol
Volume: 34
Issue: 24
Pages: 2881-2887
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
[Journal Article] Association of Low-Dose Aspirin and Survival of Women With Endometrial Cancer.2016
Author(s)
Matsuo K, Cahoon SS, Yoshihara K, Shida M, Kakuda M, Adachi S, Moeini A, Machida H, Garcia-Sayre J, Ueda Y, Enomoto T, et al.
-
Journal Title
Obstet Gynecol.
Volume: 128
Issue: 1
Pages: 127-37
DOI
Related Report
Peer Reviewed
-
[Journal Article] Allelic Imbalance in Regulation of ANRIL through Chromatin Interaction at 9p21 Endometriosis Risk Locus.2016
Author(s)
Nakaoka H, Gurumurthy A, Hayano T, Ahmadloo S, Omer WH, Yoshihara K, Yamamoto A, Kurose K, Enomoto T, Akira S, Hosomichi K,, Inoue I.
-
Journal Title
PLoS Genet.
Volume: 1
Issue: 4
Pages: 1-23
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Establishment of a Novel Histopathological Classification of High-Grade Serous Ovarian Carcinoma Correlated with Prognostically Distinct Gene Expression Subtypes.2016
Author(s)
Murakami R, Matsumura N, Mandai M, Yoshihara K, Tanabe H, Nakai H, Yamanoi K, Abiko K, Yoshioka Y, Hamanishi J, Yamaguchi K, Baba T, Koshiyama M, Enomoto T, Okamoto A, Murphy SK, Mori S, Mikami Y, Minamiguchi S, Konishi I
-
Journal Title
Am J Pathol.
Volume: 186
Issue: 5
Pages: 1103-13
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
[Journal Article] Mothers' attitudes in Japan regarding cervical cancer screening correlates with intention to recommend cervical cancer screening for daughters.2016
Author(s)
Egawa-Takata T, Ueda Y, Tanaka Y, Morimoto A, Kubota S, Yagi A, Terai Y, Ohmichi M, Ichimura T, Sumi T, Murata H, Okada H, Nakai H, Mandai M, Yoshino K, Kimura T, Saito J, Kudo R, Sekine M, Enomoto T, Horikoshi Y, Takagi T, Shimura K.
-
Journal Title
Int J Clin Oncol.
Volume: 21
Issue: 5
Pages: 962-968
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Similar protein expression profiles of ovarian and endometrial high-grade serous carcinomas.2016
Author(s)
Hiramatsu K, Yoshino K, Serada S, Yoshihara K, Hori Y, Fujimoto M, Matsuzaki S, Egawa-Takata T, Kobayashi E, Ueda Y, Morii E, Enomoto T, Naka T, Kimura T.
-
Journal Title
Br J Cancer.
Volume: 114(5)
Issue: 5
Pages: 554-561
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Venous thromboembolism, interleukin-6 and survival outcomes in patients with advanced ovarian clear cell carcinoma.2015
Author(s)
Matsuo K, Hasegawa K, Yoshino K, Murakami R, Hisamatsu T, Stone RL, Previs RA, Hansen JM, Ikeda Y, Miyara A, Hiramatsu K, Enomoto T, Fujiwara K, Matsumura N, Konishi I, Roman LD, Gabra H, Fotopoulou C, Sood AK.
-
Journal Title
Eur J Cancer
Volume: 51(14)
Pages: 1978-88
Related Report
Peer Reviewed
-
[Journal Article] Survival outcome of stage I ovarian clear cell carcinoma with lympho-vascular space invasion.2015
Author(s)
Matsuo K, Yoshino K, Hasegawa K, Murakami R, Ikeda Y, Adachi S, Hiramatsu K, Yokoyama T, Nishimura M, Sheridan TB, Enomoto T, Fujiwara K, Matsumura N, Konishi I, Fotopoulou C, Roman LD, Sood AK.
-
Journal Title
Gynecol Oncol.
Volume: 136(2)
Pages: 198-204
Related Report
Peer Reviewed
-
-
[Journal Article] CpG ODN potentiates the anti-tumor activity of anti-BST2 antibody.2015
Author(s)
Hiramatsu, K., Serada, S., Kobiyama, K., Nakagawa, S., Morimoto, A., Matsuzaki, S., Ueda,Y., Fujimoto, M., Yoshino, K., Ishii, K., Enomoto, T., Kimura, T., Naka, T.
-
Journal Title
Cancer Science
Volume: 106(10)
Issue: 10
Pages: 474-82
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Identification of novel exonic mobile element insertions in epithelial ovarian cancers.2015
Author(s)
Hayano,T., Yamada,S., Hosomichi,K., Nakaoka,H., Yoshihara,K., Adachi.S. Kashima,K., Tanaka, K and Enomoto,T.& Inoue,I.
-
Journal Title
Human Genome Variation
Volume: 2
Issue: 1
Pages: 15030-15030
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-