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The role of trophoblast or endometrial stem cell in implantation and development of trophoblasts

Research Project

Project/Area Number 15K15608
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Obstetrics and gynecology
Research InstitutionKyushu University

Principal Investigator

Kato Kiyoko  九州大学, 医学研究院, 教授 (10253527)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords絨毛細胞 / 幹細胞 / 子宮内膜 / 老化 / 絨毛幹細胞 / 着床 / 子宮内膜幹細胞 / サイトカイン
Outline of Final Research Achievements

We analyze the role of trophoblast or endometrial stem cell in implantation and development of trophoblasts. First of all, we isolated the side population cells of trophoblast cells, HTR-SVneo using FACS analysis. The proportion of next generated SP cells, a potential of serial colony formation, a long-term proliferation activity and the level of CDX2 were enhanced in SP cells compared with non-SP cells. Ly6D gene was the most overexpressed gene in SP cells and it was important for the maintenance of stemness of SP cells. Next, we analyzed the correlation between the aging signals in endometrial cells and the success of implantation. The ratio of senescence cells, the levels of p21and the ratio of G0/G1 phase of cell cycle were enhanced in the endometrial cells of the implantation failure cases. There was a difference of the secretion of inflammatory cytokines from the endometrial cells between success cases and failure cases of implantation.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (18 results)

All 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results)

  • [Int'l Joint Research] Verastem(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Verastem(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Air Pollutant Exposure within a Few Days of Delivery, and Placental Abruption in Ja-pan.2017

    • Author(s)
      Michikawa T, Morokoma S, Yamazaki S, Fukushima K, Kato K, Nitta H
    • Journal Title

      Epidemiology

      Volume: 28(2) Issue: 2 Pages: 190-196

    • DOI

      10.1097/ede.0000000000000605

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exposure to air pollutants during the early weeks of pregnancy, and placenta praevia and placenta ac-crete in the western part of Japan.2016

    • Author(s)
      Michikawa T, Morokuma S, Yamazaki S, Fukushima K, Kato K, Nitta H
    • Journal Title

      Environ Int.

      Volume: 92-93 Pages: 464-470

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An improved method for isolation of epithelial and stromal cells from the human endometrium2016

    • Author(s)
      Masuda A, Katoh N, Nakabayashi K, Kato K, Sonoda K, Kitade M, Takeda S, Hata K, Tomikawa J.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 62 Issue: 2 Pages: 213-218

    • DOI

      10.1262/jrd.2015-137

    • NAID

      130005146460

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] SNP55, a new functional polymorphism of MDM2-P2 promoter, contributes to allele-specific expression of MDM2 in endometrial cancers.2015

    • Author(s)
      Okamoto K, Tsunematsu R, Tahira T, Sonoda K, Asanoma K, Yagi H, Yoneda T, Hayashi K, Wake N, Kato K
    • Journal Title

      BMC Medical Genetics

      Volume: 16 Issue: 1 Pages: 67-67

    • DOI

      10.1186/s12881-015-0216-8

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Placental elasticity evaluation using virtual touch tissue quantification during pregnancy.2015

    • Author(s)
      Ohmaru T, Fujita Y , Sugitani M, Shimokawa M, Fukushima K, Kato K
    • Journal Title

      Placenta.

      Volume: 36 Pages: 915-920

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Endometrial Cancer Stem Cell as a Potential Therapeutic Target2015

    • Author(s)
      Kyo S, Kato K
    • Journal Title

      Semin Reprod Med.

      Volume: 33 Issue: 05 Pages: 341-349

    • DOI

      10.1055/s-0035-1563407

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of the Mechanism of TWIST1 Transcription by BHLHE40 and BHLHE41 in Cancer Cells.2015

    • Author(s)
      Asanoma K, Liu G, Yamane T, Miyanari Y, Takao T, Yagi H, Ohgami T, Ichinoe A, Sonoda K, Wake N, Kato K
    • Journal Title

      Molecular and Cellular Biology

      Volume: 35 Issue: 24 Pages: 4096-4109

    • DOI

      10.1128/mcb.00678-15

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Hyperglycemia impairs left-right axis formation and thereby disturbs heart morphogenesis in mouse embryos.2015

    • Author(s)
      Hachisuga M, Oki S, Kitajima K, Ikuta S, Sumi T, Kato K, Wake N, Meno C.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 112 Issue: 38 Pages: 5300-5307

    • DOI

      10.1073/pnas.1504529112

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Up-regulation of lymphocyte antigen 6 complex expression in side-population cells derived from a human trophoblast cell line HTR-8/SVneo.2015

    • Author(s)
      Inagaki T , Kusunoki S, Tabu K, Okabe H , Yamada I , Taga T , Matsuomoto A , Makino S , Takeda S , Kato K
    • Journal Title

      Human Cell

      Volume: 29 Issue: 1 Pages: 10-21

    • DOI

      10.1007/s13577-015-0121-7

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] Identification and characterization of human trophoblast stem-like cells.2016

    • Author(s)
      Kato K
    • Organizer
      The 102nd Annual Congress of Korean Society of Obstetrics and Gynecology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2016-09-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ISP-29-1. The expression of fibrosis related factor in villous stroma of placenta complicated with preeclampsia is induced by hypoxia and TGFβ stimulation.2016

    • Author(s)
      Ohmaru T,Asanoma K,Kondo Y,Hidaka N,Fujita Y,Kato K
    • Organizer
      第68回日本産科婦人科学会学術講演会
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Year and Date
      2016-04-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] 産婦人科における幹細胞研究とその臨床応用2016

    • Author(s)
      加藤聖子
    • Organizer
      第8回産婦人科内分泌研究会
    • Place of Presentation
      東京ガーデンパレス(東京都文京区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Identification and characterization of endometrial cancer stem-like cells.2015

    • Author(s)
      Kato K
    • Organizer
      The 4th Biennial Meeting of Asian Society of Gynecologic Oncology
    • Place of Presentation
      Seoul
    • Year and Date
      2015-11-12
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] がん治療のゲノム個別化 Precision Medicineを目指す多様な視点:がん幹細胞ー子宮体がん幹細胞の生物学的特性の解析と治療開発への展望.2015

    • Author(s)
      加藤聖子
    • Organizer
      第53回日本癌治療学会学術集会
    • Place of Presentation
      京都市
    • Year and Date
      2015-10-30
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 子宮内膜幹細胞の特性の解析2015

    • Author(s)
      加藤聖子
    • Organizer
      第18回日本IVF学会学術集会
    • Place of Presentation
      福岡市
    • Year and Date
      2015-09-27
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 子宮体癌の発生・進展機構の解明と新規治療法の開発―がん幹細胞の観点から―2015

    • Author(s)
      加藤聖子
    • Organizer
      第67回日本産科婦人科学会学術講演会
    • Place of Presentation
      横浜市
    • Year and Date
      2015-04-12
    • Related Report
      2015 Research-status Report
    • Invited

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Published: 2015-04-16   Modified: 2022-02-16  

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