Production of synthetic peptides with antimetastatic activity through inhibition of uPAR function
| Project/Area Number |
15K15609
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
吉元 千陽 奈良県立医科大学, 医学部, 助教 (00526725)
伊東 史学 奈良県立医科大学, 医学部, 助教 (20553241)
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| Research Collaborator |
KURITA Noriyuki 豊橋科学技術大学, 工学(系)研究科(研究院), 准教授
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | がん転移 / 卵巣癌 / 癌転移 |
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| Outline of Final Research Achievements |
To develop a potent inhibitor for the uPA binding, many types of peptides of amino acids were produced and their effect on the cancer invasion was investigated in the previous biochemical experiments. A computer ab initio molecular simulation has clarified that some amino acid residues of uPA play important roles in the specific binding between uPA and uPAR. In the present study, we propose KG6-Glu, KG7-Glu, KG8-Glu, KG9-Glu and KG10-Glu synthetic peptides and investigate the specific interactions and the binding affinity between uPAR and the peptides based on binding assay, Western blot, invasion and cell growth assay. We elucidated KG6-Glu peptide can bind more strongly to uPAR and propose a novel potent peptide which can inhibit the binding between uPAR and uPA efficiently. KG6-Glu peptide strongly inhibited tumor metastasis.
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Report
(3 results)
Research Products
(2 results)
