| Project/Area Number |
15K15610
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
MARUYAMA Tetsuo 慶應義塾大学, 医学部(信濃町), 准教授 (10209702)
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| Co-Investigator(Kenkyū-buntansha) |
内田 浩 慶應義塾大学, 医学部(信濃町), 講師 (90286534)
升田 博隆 慶應義塾大学, 医学部(信濃町), 講師 (80317198)
小野 政徳 慶應義塾大学, 医学部(信濃町), 共同研究員 (70348712)
荒瀬 透 慶應義塾大学, 医学部(信濃町), 共同研究員 (20348709)
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| Research Collaborator |
TAKAO Tomoka 慶應義塾大学, 医学部, 特任助教 (40612429)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 子宮筋腫 / MED12 / CRISPR/CAS9 / 生殖医学 / 幹細胞医学 / 脱細胞化 / 再細胞化 |
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| Outline of Final Research Achievements |
Uterine myometrial cells contained a small but significant amount of stem-like cells immediately after isolation from human myometrial tissues. The number of the stem-like cells, however, decreased considerably when they are cultured in vitro. Instead of myometrial stem-like cells, we introduced MED12 mutations, which are observed in approximately 70 % of uterine leiomyomas, into human myometrium cells using CRISPR/CAS9 system, and analyzed hormone-dependent cell proliferation and collagen expression. Preliminary data showed that MED12 mutation did not affect cell growth but tended to induce collagen expression. In vivo leiomyoma model system is under development.
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