| Project/Area Number |
15K15612
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
関沢 明彦 昭和大学, 医学部, 教授 (10245839)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWASHIMA Akihiro 昭和大学, 医学部, 助教 (10783376)
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| Research Collaborator |
NAKABAYASHI Makoto 昭和大学, 医学部, 助教
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | cell free DNA / MPS法 / 婦人科悪性腫瘍 / CNV / 染色体不安定性 / 腫瘍マーカー / 癌バイオマーカー / 遺伝子産物 / 子宮体癌 / 子宮頸癌 / 卵巣癌 |
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| Outline of Final Research Achievements |
DNA derived from tumor cells with chromosomal instability is released into the peripheral blood. It is reported that abnormal copy number variations (CNV) is present in peripheral blood cell-free DNA. Peripheral blood cell-free DNA is analyzed to investigate the CNV at the chromosomal level in the cancer-bearing state.
Cell free DNA was extracted from the plasma of patients with preoperative gynecological malignancies after informed consent. We analyzed CNV in peripheral blood cell-free DNA. The samples were obtained from 64 patients. CNV in all chromosomes were detected in 4cases. CNV in partial chromosomes were detected in 19 patients.It was thought that malignant tumor could be detected by analyzing cell - free DNA of patients with malignant tumor.
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