Development of a novel method for ovarian stimulation based on suppression of PDE5 activity in ovarian granulosa cells
| Project/Area Number |
15K15613
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高江 正道 聖マリアンナ医科大学, 医学部, 講師 (00621301)
吉岡 伸人 聖マリアンナ医科大学, 医学部, 助教 (10468928)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 卵胞発育 / PDE5 / cGMP / 不妊治療 / phosphodiesterase 5 |
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| Outline of Final Research Achievements |
Currently, there is no effective treatment for stimulation of follicle growth in infertility patients who show low-response to FSH stimulation due to aging etc. FSH stimulates follicle growth through production of cGMP as a down-stream factor and hosphodiesterase 5 (PDE5) acts to metabolize cGMP to attenuate FSH signal. In this study, we attempt to develop a new method to induce follicle growth by suppression of PDE5 activity. Inhibitors of PDE5, Sildenafil and Tadalafil increased the response to FSH stimulation in ovarian granulosa cells by inhibiting PDE5 activity, leading to stimulation of follicle growth. We also confirmed the normality of mouse eggs obtained from ovaries after ovarian stimulation using PDE5 inhibitors.
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Report
(4 results)
Research Products
(78 results)
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[Journal Article] In Vitro Activation of Follicles and Fresh Tissue Auto-transplantation in Primary Ovarian Insufficiency Patients.2016
Author(s)
Zhai J, Yao G, Dong F, Bu Z, Cheng Y, Sato Y, Hu L, Zhang Y, Wang J, Dai S, Li J, Sun J, Hsueh AJ, Kawamura K, Sun Y
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Journal Title
J Clin Endocrinol Metab
Volume: 101
Issue: 11
Pages: 4405-4412
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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