| Project/Area Number |
15K15622
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Akiyoshi Kazunari 京都大学, 工学研究科, 教授 (90201285)
Mazda Osam 京都府立医科大学, 医学研究科, 教授 (00271164)
Nakano Hiroshi 京都府立医科大学, 医学研究科, 講師 (00405309)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | アレルギー |
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| Outline of Final Research Achievements |
We developed thirty-one kinds of IL-21 artificial transcription factor based on Crispr-Cas9 system. Fifteen kinds of IL-21 artificial transcription factor enhanced IL-21 promoter activities in reporter assay. Then we asked whether IL-21 artificial transcription factor induces mRNA expression. Unfortunately, each IL-21 artificial transcription factor failed to induce IL-21 mRNA. So, we examined wether the mixture of IL-21 artificial transcription factor transcribes IL-21 mRNA, IL-21 expression strongly induced in 3T3 cells (even in undetectable level by qRT-PCR). The present study may lead to a novel therapeutic method against allergic disorders.
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