In vitro "virtual biopsy" of cochlear cells by using Wolfram syndrome patients derived hiPSCs
Project/Area Number |
15K15624
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Otorhinolaryngology
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Research Institution | Keio University |
Principal Investigator |
Fujioka Masato 慶應義塾大学, 医学部(信濃町), 講師 (70398626)
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Co-Investigator(Kenkyū-buntansha) |
小川 郁 慶應義塾大学, 医学部, 教授 (00169179)
大石 直樹 慶應義塾大学, 医学部, 講師 (10348740)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 疾患iPS創薬 / WFS1 / Wolfram症候群 / 遺伝性難聴 / コモンマーモセット / 橋渡し研究 / ヒトiPS細胞 |
Outline of Final Research Achievements |
We identified the expression pattern of WFS1 gene in the primate cochlea using a small non-human primate common marmoset (Suzuki N, 2016). In marmosets, unlike rodents, WFS1 was also expressed in the basal cell of stria vascularis, where severe atrophy was reported in the temporal bone of Wolfram syndrome patients. Based on these findings, we generated WFS1 positive cochlear cells from human iPSCs and its function was analyzed.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Prevalence of TECTA mutation in patients with mid-frequency sensorineural hearing loss.2017
Author(s)
Yamamoto N, Mutai H, Namba K, Morita N, Masuda S, Nishi Y, Nakano A, Masuda S, Fujioka M, Kaga K, Ogawa K, Matsunaga T.
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Journal Title
Orphanet J Rare Dis
Volume: 25;12(1)
Issue: 1
Pages: 157-157
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Sustained Effect of Hyaluronic Acid in Subcutaneous Administration to the Cochlear Spiral Ganglion.2016
Author(s)
Inagaki Y, Fujioka M, Kanzaki S, Watanabe K, Oishi N, Itakura G, Yasuda A, Shibata S, Nakamura M, Okano HJ, Okano H, Ogawa K.
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Journal Title
PLoS One in press
Volume: 11
Issue: 4
Pages: e0153957-e0153957
DOI
Related Report
Peer Reviewed / Open Access
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