| Project/Area Number |
15K15626
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Tohoku University |
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Principal Investigator |
Abe Toshiaki 東北大学, 医学系研究科, 教授 (90191858)
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| Co-Investigator(Kenkyū-buntansha) |
梶 弘和 東北大学, 工学研究科, 准教授 (70431525)
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| Co-Investigator(Renkei-kenkyūsha) |
Nishizawa Matsuhiko 東北大学, 大学院工学系研究科, 教授 (20273592)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 網膜色素上皮細胞 / マイクロ流路 / 活性酸素 / 低酸素 / ＶＥＧＦ |
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| Outline of Final Research Achievements |
We investigated the feasibility of the production of bio-batteries using micro-channel or eye ball by measuring active oxygen. It was possible to fabricate a porous polydimethylsiloxane (PDMS) film having an array of 10 micron pores and a PDMS mold having a flow path structure and culture retinal pigment epithelial cells (ARPE) in FY2015. In FY 2016, we could co-cultivate ARPE with HUVEC and found HUVEC migrate to the ARPE side, especially in some stress conditions. It turned out the possibility that mitochondria was involved in cultured cell death. Since the change in the generation of active oxygen was seen in mitochondria, we think there is a possibility to create the bio-battery, our final objective of production.
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