Analysis of molecular basis of retinal glial cells and their roles in retinal degeneration diseases
Project/Area Number |
15K15628
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Ophthalmology
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Research Institution | The University of Tokyo |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 網膜 / ミュラーグリア / マイクログリア / 変性 / 遺伝子発現 / ヒストンメチル化 / ミューラーグリア / 視細胞変性 |
Outline of Final Research Achievements |
We established technic to purify photoreceptor, Mueller glia, and microglia by using multiple cell surface antigens and a cell sorter. Then, these retinal subsets were purified from normal and photoreceptor-degenerated retina, and RNA-seq was performed using the fractions. Genes specifically up-regulated in each retinal subtype were identified by bio-informatics technics. Time course of gene expression was examined, and interaction of the subtypes through cytokines and chemokines during retinal degeneration was examined from the results.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Conditional rod photoreceptor ablation reveals Sall1 as a microglial marker and regulator of microglial maturation2016
Author(s)
Koso, H., Tsuhako A., Lai, C. Y., Baba, Y., Otsu, M., Ueno, K., Nagasaki, M., Suzuki, Y., Watanabe, S.
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Journal Title
Glia
Volume: 64
Pages: 2005-2024
DOI
Related Report
Peer Reviewed
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[Journal Article] Expression pattern of Ccr2 and Cx3cr1 in inherited retinal degeneration2015
Author(s)
Kohno, H, Koso, H, Okano, K, Sundermeier, TR, Saito, S., Watanabe, S., Hiroshi Tsuneoka, H., Sakai, T.
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Journal Title
Neuroinflammation
Volume: 12
Pages: 1-12
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Roles Histone H3K27 tri-methylase Ezh2 in retinal proliferation and differentiation2015
Author(s)
Iida, A., Iwagawa, T., Baba, Y., Satoh, S., Mochizuki, Y., Nakauchi, H. Furukawa, T., Koseki, H., Murakami, A., Watanabe, S.
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Journal Title
Developmental Neurobiology
Volume: 111
Pages: 3751-3756
DOI
Related Report
Peer Reviewed
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