| Project/Area Number |
15K15636
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
Tanihara Hidenobu 熊本大学, 大学院生命科学研究部(医), 教授 (60217148)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 4次元イメージング / MCP-1 / 緑内障 / 生体分子 / 創傷治癒 / 生理活性 |
|---|
| Outline of Final Research Achievements |
The conditioned medium of conjunctival fibroblast cells increased migration of monocyte THP-1 cells. On the other hand, the conditioned medium of myofibroblast cells presented lower chemotactic activity of THP-1 migration in a comparison with medium of fibroblast. These migrations were inhibited by CCR2 inhibitor and MCP-1 neutralizing antibody. Additionally, the concentration of MCP-1 was decreased in conditioned medium of myofibroblast compared with conditioned medium of fibroblast.
We performed 4-dimentional live imaging using LysM-eGFP mice, and found that the number of LysM positive cell and the cell velocities were increased by MCP-1 administration to conjunctival surface. The effects of MCP-1 on conjunctiva, such as increased cell number and cell velocities, were suppressed by CCR2 inhibitor. These results indicated that LysM positive cells were accumulated and activated by MCP-1 through CCR2 receptor at conjunctiva.
|
