| Project/Area Number |
15K15653
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
AKITA Sadanori 長崎大学, 病院(医学系), 助教 (90315250)
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| Co-Investigator(Kenkyū-buntansha) |
YOHIMOTO Hiroshi 長崎大学, 病院(医学系), 助教 (90513309)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUSE Michiko 長崎大学, 原爆後障害医療研究所, 助教 (30533905)
NAKAZAWA Yuka 長崎大学, 原爆後障害医療研究所, 助教 (00533902)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | ケロイド / 放射線 / 難治創傷 / 幹細胞 / 創傷治癒 / 放射線障害 / 増殖性疾患 / 脂肪由来幹細胞 |
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| Outline of Final Research Achievements |
Treatment of critical limb ischemia is sometimes difficult due to the patient’s condition. The hindlimbs of Sprague-Dawley rats, after 20 Gy X-ray radiation and surgical occlusion, were divided with superficial fascial flap, 5.0 × 106 adipose-derived stromal/stem cell (ASC) and both combined. There were no significant differences between the flap alone and ASC alone treatment groups. Wound healing was significantly increased in the combined treatment at 1 week and there was no wound by 2 weeks except for the no flap and no ASCs group. Von Willbrand factor, blood vessel, showed a significant increase in the combined treatment group, both at 1 week and 2 weeks. In the cell tracking, at 2 weeks, the GFP-tagged ASCs were significantly more positive in the no flap group than in the flap group. ASCs may be a potent cell source in radiated and occluded limbs by enhancing tissue blood flow and blood vessel density.
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