Lineage tracing of adipose-derived stem/stromal cells in fat grafts.

• Project/Area Number: 15K15654
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Jichi Medical University
• Principal Investigator: Uda Hirokazu 自治医科大学, 医学部, 准教授 (20337306)
• Co-Investigator(Kenkyū-buntansha): 須永 中 自治医科大学, 医学部, 講師 (00406117)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 脂肪移植 / 系譜追跡 / 脂肪由来幹細胞

Outline of Final Research Achievements

We established Prx1Cre;Ai14 mice in which Prx1-expressing cells were genetically marked with tdTomato. Adipose-derived stem/stromal cells (ADSCs) from Prx1Cre;Ai14 mice expressed tdTomato. Using the transgenic mice, we transplanted fat grafts from wild mice to wild mice with tdTomato positive ADSCs, and analyzed the grafts with immunofluorescent imaging.
In the transplanted grafts, tdTomato-positive cells partially expressed perilipin or CD31, suggesting ADSCs contributed to regeneration of adipocytes and endothelial cells.