| Project/Area Number |
15K15663
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
Suzuki Takao 京都大学, 医学研究科, 客員研究員 (40328810)
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| Co-Investigator(Kenkyū-buntansha) |
平川 慶子 日本医科大学, 医学部, 助手 (30165162)
小池 薫 京都大学, 医学研究科, 教授 (10267164)
佐藤 格夫 愛媛大学, 医学系研究科, 寄附講座教授 (30409205)
金涌 佳雅 日本医科大学, 医学(系)研究科(研究院), その他 (80465343)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 敗血症 / 敗血症関連脳症 / 脳脊髄液 / ＮＭＲ / NMR解析 / NMR |
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| Outline of Final Research Achievements |
We collected cerebrospinal fluid from rat septic models, following sepsis induction by intraperitoneal administration of LPS (lipopolysaccharide). Then, the cerebrospinal fluid sample was subjected to proton (1 H) NMR measurement using a 7 tesla (300 MHz) FT-NMR apparatus (JEOL). We pattern-recognized this NMR spectrum and spectral data of a single pulse was analyzed by partial least squares regression (PLS-DA). The NS group (control group; administered physiological saline), LPS 2 group (administered 2 mg/kg LPS), and LPS 10 group (administered 10 mg/kg LPS) could not be clustered on the score plot. However, results from the analysis of the T2 spectrum data by PLS-DA suggested that there was a possibility of distinguishing between the LPS 2 and 10 groups. We confirmed that these three groups were clustered on the score plot to a certain extent.
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