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Development of novel methods to regulate only papthologically activated osteoclasts: A novel Concept for the Osteoclast Activation

Research Project

Project/Area Number 15K15677
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionKyushu University

Principal Investigator

KUKITA TOSHIO  九州大学, 歯学研究院, 教授 (70150464)

Co-Investigator(Kenkyū-buntansha) 久木田 明子  佐賀大学, 医学部, 准教授 (30153266)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords破骨細胞 / 病的活性化 / 病的骨破壊 / 膜表面分子 / プロテオミクス / 酸感受性蛍光プローブ / 病的骨吸収
Outline of Final Research Achievements

The purpose of this study is to search and find membrane molecules specific to the pathologically activated osteoclasts (PAOC), which acquired the highest bone destruction activity. As osteoclasts formed in the presence of IL-1β showed extremely high proton-secretion and bone resorbing activity, we have regarded these osteoclasts as PAOC. Data showed a marked suppression in the expression of molecules regulating terminal differentiation of normal osteoclasts and activation of integrinβ3. By use of the analysis of the cell surface proteins, we have detected the cell surface molecule specifically expressed in PAOC. Through this study, we could establish the experimental basis for the development of novel therapy targeting only PAOC.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (20 results)

All 2017 2016 2015 2014

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Acknowledgement Compliant: 2 results,  Open Access: 5 results) Presentation (13 results) (of which Int'l Joint Research: 4 results) Book (1 results)

  • [Journal Article] Osteoblasat-derived Laminin-332 is a novel negative regulator of osteoclastogenesis in bone microenvironments2017

    • Author(s)
      Uehara N, Kukita A, Nakamura-Kyumoto Y, Yamaza T, Yasuda H, Kukita T
    • Journal Title

      Laboratory Investigation

      Volume: 98

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Extremely high expression of antisense RNA for Wilms’ Tumor 1 in active osteoclasts: Suppression of Wilms’ Tumor 1 protein expression during osteoclastogenesis.2016

    • Author(s)
      Li YJ, Kukita A, Kyumoto-Nakamura Y, Kukita T.
    • Journal Title

      American Journal of Pathology

      Volume: 186(9) Issue: 9 Pages: 2317-2325

    • DOI

      10.1016/j.ajpath.2016.05.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Interferon-gamma improves impaired dentinogenic and immunosuppressive functions of irreversible pulpitis-derived human dental pulp stem cells.2016

    • Author(s)
      Sonoda S, Yamaza H, Ma L, Tanaka Y, Tomoda E, Aijima R, Nonaka K, Kukita T, Shi S, Nishimura F, Yamaza T.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 19286-19286

    • DOI

      10.1038/srep19286

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Membrane Nanotube Formation in Osteoclastogenesis.2015

    • Author(s)
      Kukita T., Takahahsi A., Zhang J-Q., Kukita A.
    • Journal Title

      Kurt Pfannkuche (ed), Cell Fusion: Overviews and Methods, Methods in Molecular Biology

      Volume: 1313 Pages: 193-202

    • DOI

      10.1007/978-1-4939-2703-6_14

    • ISBN
      9781493927029, 9781493927036
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibition of RANKL-dependent cellular fusion in pre-osteoclasts by amiloride and a NHE10-specific monoclonal antibody.2015

    • Author(s)
      Mine Y, Shuto T, Nikawa H, Kawai T, Ohara M, Kawahara K, Ohta K, Kukita T, Terada Y, Makihira S.
    • Journal Title

      Cell Biol Int.

      Volume: 39 Issue: 6 Pages: 696-709

    • DOI

      10.1002/cbin.10447

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of osteoclastogenesis through Tim-3: possible involvement of the Tim-3/galectin-9 system in the modulation of inflammatory bone destruction.2014

    • Author(s)
      K. Moriyama, A. Kukita, Y-J. Li, N. Uehara, J-Q Zhang, I. Takahashi, T. Kukita
    • Journal Title

      Lab. Invest

      Volume: 94(11) Issue: 11 Pages: 1200-1211

    • DOI

      10.1038/labinvest.2014.107

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ラミニン-332の骨芽細胞での発現と破骨細胞分化制御2017

    • Author(s)
      上原範久、久木田明子、久本由香里、保田尚孝、久木田敏夫
    • Organizer
      第122回日本解剖学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Galectin-9による破骨細胞分化抑制因子MafBの発現制御2016

    • Author(s)
      久本由香里、上原範久、久木田明子、山座孝義、久木田敏夫
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 病的に活性化された破骨細胞:形成条件の検討と蛍光プローブを用いた骨吸収イメージング2016

    • Author(s)
      白鳥卓麻、久木田明子、上原範久、久本由香里、張旌旗、山座孝義、古谷野潔、久木田敏夫
    • Organizer
      第58回歯科基礎医学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 破骨細胞形成過程における細胞融合とサイトキネシスの形態学的解析2016

    • Author(s)
      張旌旗、高橋良、白鳥卓麻、久木田明子、山座孝義、久木田敏夫
    • Organizer
      第58回歯科基礎医学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 口唇口蓋裂患児由来乳歯幹細胞の解析2016

    • Author(s)
      山座孝義、山座治義、園田聡一郎、友田恵利佳、田中陽介、上原範久、久本由香里、野中和明、久木田敏夫
    • Organizer
      第58回歯科基礎医学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Galectin-9による破骨細胞分化制御と転写因子MafBの発現制御メカニズムの解明2016

    • Author(s)
      久本由香里、上原範久、久木田明子、久木田敏夫
    • Organizer
      第34回日本骨代謝学会学術集会/第3回アジア太平洋骨代謝学会議
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウス骨転移性乳癌細胞由来エクソソームは破骨細胞分化を促進する2016

    • Author(s)
      上原範久、久本由香里、久木田明子、久木田敏夫
    • Organizer
      第34回日本骨代謝学会学術集会/第3回アジア太平洋骨代謝学会議
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] zBTB蛋白質LRF/OCZFの破骨細胞分化における機能と骨吸収中の破骨細胞における発現の解析2016

    • Author(s)
      徐祥赫、白木誠、蒲原麻菜、菖蒲池健夫、久木田敏夫、久木田明子
    • Organizer
      第34回日本骨代謝学会学術集会/第3回アジア太平洋骨代謝学会議
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 病的骨破壊の特異的制御を目指して:炎症性サイトカイン刺激下で形成される破骨細胞は病的に活性化された破骨細胞か?2016

    • Author(s)
      白鳥卓麻、上原範久、久本由香里、久木田明子、徐祥赫、古谷野潔、久木田敏夫
    • Organizer
      第34回日本骨代謝学会学術集会/第3回アジア太平洋骨代謝学会議
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 活発に機能する破骨細胞におけるWilms' Tumor-1 (WT1)アンチセンスRNAの高発現と破骨細胞分化制御2016

    • Author(s)
      久木田敏夫、李銀姫、久本由香里、久木田明子
    • Organizer
      第72回日本解剖学会 九州支部学術集会
    • Place of Presentation
      長崎
    • Related Report
      2016 Annual Research Report
  • [Presentation] ラミニン322によるRANK発現調節とマクロファージ-破骨細胞分化転換の制御2015

    • Author(s)
      上原範久、久木田明子、久本由香里、保田尚孝、久木田敏夫
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] Galectin-9による破骨細胞分化制御メカニズムの解明:NFATc1の発現を抑制する転写因士MafB及び IRF-8の関与2015

    • Author(s)
      久本由香里、上原範久、久木田明子、久木田敏夫
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] Ndd4ユビキチンリガーゼ結合タンパク質Pmepa1の破骨細胞の分化と機能における新たな役割2015

    • Author(s)
      徐祥赫、舟久保立、白木誠、久木田敏夫、久木田明子
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Book] INVOLVEMENT OF DEOXYADENOSINE AND ADENOSINE DEAMINASE IN THE METHOTREXATE-INDUCED SUPPRESSION OF INFLAMMATORY BONE DESTRUCTION*2015

    • Author(s)
      Peng-Fei Qu, Akiko Kukita, Yin-Ji Li, Kanako Moriyama, Lin Lei and Toshio Kukita
    • Total Pages
      23
    • Publisher
      Novs Science Publisher, Inc. NewYork
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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