Functional analysis of stem cell in salivary gland aging

• Project/Area Number: 15K15678
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Showa University
• Principal Investigator: Mishima Kenji 昭和大学, 歯学部, 教授 (50275343)
• Co-Investigator(Kenkyū-buntansha): 安原 理佳 昭和大学, 歯学部, 講師 (20453649) ; 河野 葉子 昭和大学, 歯学部, 准教授 (40195681) ; 田中 準一 昭和大学, 歯学部, 助教 (40710166)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 唾液腺 / 再生 / 老化 / 幹細胞 / CD133 / 幹細胞老化 / LRCs / 再生医療 / 唾液腺老化

Outline of Final Research Achievements

Dry mouth is one of causes to promote various diseases including dental caries, eating and swallowing disorders, and aspiration pneumonia, which, in severe cases, results in remarkable decrease of quality of life (QOL). Dry mouth is known to be caused by Sjogren’s syndrome, irradiation, medication, and ageing. The aim of this research is to clarify whether ageing of salivary gland stem cells is involved in salivary gland atrophy found in old people. The salivary gland stem cells of young mice (six weeks old) and old mice (80~90 weeks old) were identified as CD133-positive cells using flow cytometry and characterized. Consequently, the frequency of salivary gland stem cells was lower in submandibular glands of old mice than that in the young mice. In addition, salisphere-forming capacity was also decreased in submandibular glands of old mice compared with that in young mice. These results suggested that stem cell ageing was possibly involved in ageing-induced salivary gland atrophy.

Research Products

• [Journal Article] The Role of the Slc39a Family of Zinc Transporters in Zinc Homeostasis in Skin (2018)
  • Author(s): Bin Bum-Ho、Hojyo Shintaro、Seo Juyeon、Hara Takafumi、Takagishi Teruhisa、Mishima Kenji、Fukada Toshiyuki
  • Journal Title: Nutrients Volume: 10 Issue: 2 Pages: 219-219
  • DOI: 10.3390/nu10020219
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Establishment of mouse gingival junctional epithelial cell line using a bioengineered tooth (2018)
  • Author(s): Seki T, Aizawa R, Tanaka J, Yajima-Himuroa S, Kato M, Tanaka K, Mishima K, Yamamoto M.
  • Journal Title: Biochem Biophys Res Commun Volume: 047 Issue: 1 Pages: 167-172
  • DOI: 10.1016/j.bbrc.2018.02.047
  • Peer Reviewed / Open Access
• [Journal Article] Requirement of Zinc Transporter SLC39A7/ZIP7 for Dermal Development to Fine-Tune Endoplasmic Reticulum Function by Regulating Protein Disulfide Isomerase. (2017)
  • Author(s): Bin BH, J. Bhin, J. Seo, SY. Kim, E. Lee, K. Park, DH. Choi, T. Takagishi, T. Hara, D. Hwang, H. Koseki, Y. Asada, S. Shimoda, K. Mishima, T. Fukada.
  • Journal Title: Journal of Investigative Dermatology Volume: 137 Issue: 8 Pages: 1682-1691
  • DOI: 10.1016/j.jid.2017.03.031
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 唾液腺発生におけるSox9の機能解析 (2016)
  • Author(s): 田中準一, 大庭 伸介, 馬渕 洋, 安原理佳, 入江太朗, 福島美和子, 河野葉子, 美島健二
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-11-30
• [Presentation] Sox9 is involved in the ability to self-renew of stem-like cells in murine salivary gland. (2016)
  • Author(s): 田中準一, 馬渕 洋, 安原理佳, 入江太朗, 福島美和子, 河野葉子, 美島健二 : Sox9を介したマウス唾液腺組織幹細胞の機能解析
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2016-05-12
• [Presentation] Sox9を介したマウス唾液腺由来CD133陽性細胞の機能解析 (2016)
  • Author(s): 田中準一、馬渕洋、安原理佳、入江太朗、福島美和子、河野葉子、美島健二
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2016-03-17