Search for molecular signatures that discriminate between tendon and ligament tissue
Project/Area Number |
15K15679
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Morphological basic dentistry
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Research Institution | Tsurumi University |
Principal Investigator |
NIFUJI AKIRA 鶴見大学, 歯学部, 教授 (00240747)
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Co-Investigator(Kenkyū-buntansha) |
江面 陽一 東京医科歯科大学, 難治疾患研究所, 准教授 (50333456)
荒木 良子 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 放射線障害治療研究部, チームリーダー(定常) (40392211)
中島 和久 鶴見大学, 歯学部, 講師 (90252692)
島田 明美 鶴見大学, 歯学部, 講師 (00339813)
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Project Period (FY) |
2015-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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Keywords | 腱組織 / 靱帯組織 / 転写産物 / 力学的負荷 / 腱細胞 / 遺伝子発現 / 細胞分化 / 靱帯細胞 / エピジェネティクス |
Outline of Final Research Achievements |
Our aging population will increase the prevalence of musculoskeletal diseases, so the importance of tendon and ligament tissue that support muscles and skeletons is recognized. Tendon and ligament tissues are morphologically similar, but their functional properties seem to be different. This study aimed to search for molecular signatures that can discriminate molecular differences between the two issues. By dissecting mouse tendon and ligament tissues, we compared whole transcripts of two tissue by RNA sequencing. We also compared the transcripts which were expressed in the primary cells isolated from those tissues. We found groups of transcripts differentially expressed between two tissue. The expressions of some transcripts changed after mechanical loading, and treatment with TGFb2 resulted in changes of those transcripts in tenocytes. Thus those newly identified genes could be functional in tendon and/or ligament tissues.
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Academic Significance and Societal Importance of the Research Achievements |
腱組織は骨と筋肉を連結する組織であり、筋肉からの収縮-伸張サイクルの影響を受け長さが変化する。一方靱帯組織は関節を形成する骨と骨を連結し運動を制御する制動器官としての働きをもつ。ともに線維性結合組織を主たる成分とし、力学的負荷に対応する構造を持つが、それらを区別できる分子(群)があるか否かについては不明である。本研究で行った転写産物の解析から、いくつかの分子(群)が、異なる発現量を示すこと、それらが力学的負荷に対応する変化を示すことが解った。これらの知見は腱・靱帯に関わる疾患への基礎的基盤に繋がると考えられる。
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Report
(5 results)
Research Products
(45 results)
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[Journal Article] Annexin A5 involvement in bone overgrowth at the enthesis2018
Author(s)
Shimada Akemi, Ideno Hisashi, Arai Yoshinori, Komatsu Koichiro, Wada Satoshi, Yamashita Teruhito, Amizuka Norio, Poschl Ernst, Brachvogel Bent, Nakamura Yoshiki, Nakashima Kazuhisa, Mizukami Hiroaki, Ezura Yoichi, Nifuji Akira
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Journal Title
Journal of Bone and Mineral Research
Volume: 印刷中
Issue: 8
Pages: 1532-1543
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Essential roles of G9a in cell proliferation and differentiation during tooth development.2017
Author(s)
Kamiunten T, Ideno H, Shimada A, Arai Y, Terashima T, Tomooka Y, Nakamura Y, Nakashima K, Kimura H, Shinkai Y, Tachibana M, Nifuji A
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Journal Title
Experimental Cell Research.
Volume: 印刷中
Issue: 2
Pages: 170-180
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Annexin A5 prevents force-mediated bone ridge overgrowth at the enthesis.2017
Author(s)
Shimada A., Arai Y., Komatsu K., Wada S., Ideno H., Nakashima K., Yamashita T., Ezura Y., Amizuka N., Poumlschl E., Brachvogel B., Nakamura Y., Nifuji A.
Organizer
Spring Harbor Lab meeting, Annexins: 9th International Conference on the Annexins
Related Report
Int'l Joint Research
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[Presentation] Histone methyltransferase G9a is essential to progress osteoblastic differentiation in vitro, and skull bone formation in vivo2015
Author(s)
Ideno H, Komatsu K, Shimada A, Kamiunten T, Arai Y, Nakashima K, Araki R, Nakamura Y, Abe M, Tachibana M, Kimura H, Nifuji A.
Organizer
2015 ASCB Annual Meeting
Place of Presentation
San Diego Convention Center, San Diego, CA, USA
Year and Date
2015-12-12
Related Report
Int'l Joint Research
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[Presentation] Essential roles of H3K9MTase G9a during tooth development2015
Author(s)
Kamiunten T, Shimada A, Ideno H, Nakamura Y, Kimura H, Tachibana M, Nakashima K, Nifuji A
Organizer
2015 ASCB Annual Meeting
Place of Presentation
San Diego Convention Center, San Diego, CA, USA
Year and Date
2015-12-12
Related Report
Int'l Joint Research
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[Presentation] Deletion of G9a, histone methyltransferase, causes impaired bone formation.2015
Author(s)
Ideno H, Komatsu K, Shimada A, Kamiunten T, Arai Y, Nakashima K, Araki R, Nakamura Y, Abe M, Tachibana M, Kimura H, Nifuji A
Organizer
ASM 2015, Joint ANZBMS, MEPSA and MBSANZ Annual Scientific Meeting
Place of Presentation
Hotel Grand Chancellor Hobart,Tasmania, Australia
Year and Date
2015-11-01
Related Report
Int'l Joint Research
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[Presentation] Annexin A5 inhibits bony outgrowth at tendon/ligament insertion sites.2015
Author(s)
Shimada A, Arai Y, Wada S, Ideno H, Kamiunten T, Nakashima K, Komatsu K, Yamashita T, Ezura Y, Amizuka N, Pöschl E, Brachvogel B, Nakamura Y, Nifuji A
Organizer
ASBMR 2015 annual meeting
Place of Presentation
Washington State Convention Center, Seattle, WA, USA
Year and Date
2015-10-09
Related Report
Int'l Joint Research
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[Presentation] Histone 3 lysine 9 methyltransferase G9a is essential for the growth and differentiation of tenocytes.2015
Author(s)
Wada S, Ideno H, Shimada H, Kamiunten T, Nakamura Y, Nakashima K, Kimura H, Tachibana M, Nifuji A.
Organizer
ASBMR 2015 annual meeting
Place of Presentation
Washington State Convention Center, Seattle, WA, USA
Year and Date
2015-10-09
Related Report
Int'l Joint Research
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