| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Vertebrates have the cells which receive the mechanical force from the outer world and translate to the intracellular signaling. However, it is not clear the strength of mechanical stimulation at the local area of the cell and how the signals transduce from cell to cell because these mechanosensory cells are often embedded in the hard tissues like bone. So, we developed the non-contact method to provide mechanical stress in the specified area of the cell by using visible light. We made some light-activatable protein pairs containing LOV domain (light accepting module) and actin-depolymerizing proteins. The expressing vectors of light-activatable proteins were transfected into the MC3T3-E1 cells. The irradiation of blue light to the cells has resulted in a decrease of the actin filament. As this effect is not so remarkably, the improvement of light-activatable proteins is needed.
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