Generation of osteocyte-specific Cre mouse
| Project/Area Number |
15K15682
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
Hayashi Mikihito 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (50581914)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Creマウス / 骨細胞 / 骨生物学 / 細胞・組織 |
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| Outline of Final Research Achievements |
Growing evidences suggest that osteocytes, which constitute a considerable fraction of bone cells, play a key role not only in the regulation of bone homeostasis, but also in the maintenance of whole body as an endocrine cell. Currently, Dmp1-Cre transgenic mice are widely used as an osteocyte-specific Cre mouse line to achieve osteocyte-specific gene deletion. However, recent reports suggest that Dmp1-Cre is also expressed in mature osteoblasts, muscle, certain brain cells, gastric and intestinal mesenchymal cells, as well as osteocytes. In this study, by performing transcriptomic analysis of osteoblastogenesis/osteocytogenesis and Dmp1-positive/negative calvarial cells derived from Dmp1-Cre CAG-CAT-EGFP mice, we identified the gene which exclusively expressed in osteocytes and generated truly osteocyte-specific Cre mice.
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Report
(3 results)
Research Products
(1 results)
