| Project/Area Number |
15K15686
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
Eijiro Jimi 九州歯科大学, 歯学部, 教授 (40276598)
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| Co-Investigator(Kenkyū-buntansha) |
古株 彰一郎 九州歯科大学, 歯学部, 准教授 (30448899)
福島 秀文 東北大学, 歯学研究科, 准教授 (70412624)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | NF-kB / 活性化機構 / NF-κB / リン酸化 / p65サブユニット |
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| Outline of Final Research Achievements |
To investigate the physiological role of the serine residue at position 534 of the NF-κB, p65 subunit, we generated knock-in mice (S534A mice) in which the serine residue at position 534 was substituted with alanine. We established mouse embryonic fibroblasts (MEF) derived from wild-type and S534A mice. When we treated with TNFα, the nuclear translocation of p65 persisted for a longer time and enhanced transcriptional activity of NF-κB in S534A cells compared with wild-type cells. These results led us to, thought that there is phosphorylation-specific negative feedback mechanism of p65 S534. Therefore, we identified a novel molecule binding specifically to phosphorylation of p65 S534, and further revealed a new regulatory mechanism by NF-κB.
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