| Project/Area Number |
15K15699
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Conservative dentistry
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
OKIJI Takashi 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (80204098)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 歯学 / 歯内治療学 / 歯髄 / 修復象牙質 / GaAlAsレーザー / osteopontin / dentin matrix protein 1 / osteopntin / 修復象牙質形成 |
|---|
| Outline of Final Research Achievements |
GaAlAs laser irradiation to the rat molar is known to induce reparative dentin formation under noninfectious condition, and seems a useful experimental model for the investigation of the mechanisms of reparative dentinogenesis. Thus, this study aimed to analyze immunolocalization and mRNA expression of dentin matrix protein 1 (DMP1) and osteopontin in this model, in order to examine the involvement of these proteins in the process of reparative dentinogenesis. Results demonstrated that osteopontin and DMP1 mRNA expression levels were upregulated prior to the formation of reparative dentin, and that colocalization of these molecules was detected at the site at which this tissue first appeared (the border between primary and reparative dentin). These findings suggest that osteopontin and DMP1 are involved in the differentiation of newly-generated odontoblast-like cells and subsequent induction of reparative dentinogenesis.
|
