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UVA-activated riboflavin inhibits demineralization of human dentin

Research Project

Project/Area Number 15K15700
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Conservative dentistry
Research InstitutionOsaka University

Principal Investigator

Hayashi Mikako  大阪大学, 歯学研究科, 教授 (40271027)

Co-Investigator(Renkei-kenkyūsha) MINOSHIMA Koji  大阪大学, 大学院工学研究科, 教授 (50174107)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords歯科保存学 / 根面う蝕 / コラーゲン / 紫外線 / リボフラビン / 象牙質 / う蝕 / 架橋 / 保存修復学 / 分子間架橋
Outline of Final Research Achievements

Flexural strength is the highest in the group of 0.1% riboflavin exposure and UVA irradiated with 1600 mW/cm2 for 10min, which was significantly higher than control group. Raman spectra showed that a C-C bond in a proline ring was amplified, and the ratio between C-N bond and N-H bond was changed by the riboflavin/UVA treatment. SDS-PAGE analysis suggested that the molecular weight of collagen was increased after the riboflavin/UVA treatment due to new collagen crosslinking.
In μCT analysis, the amount of mineral loss after demineralization in the RF/UVA group was significantly lesser than the control group. In SDS-PAGE analysis, the collagen after the collagenase degradation was less soluble in the riboflavin/UVA group than in the control group because of new collagen crosslinking promoted by a radical reaction.
Riboflavin/UVA treatment was effective in enhancing the acid and enzymatic resistance of human dentin by producing new collagen crosslinking.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (7 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 7 results,  Open Access: 5 results,  Acknowledgement Compliant: 4 results) Presentation (11 results) (of which Int'l Joint Research: 3 results,  Invited: 6 results)

  • [Int'l Joint Research] 台湾国立大学(台湾)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] ミネソタ大学(米国)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] 第四医科大学(中国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Comprehensive analyses of how tubule occlusion and advanced glycation end-products diminish strength of aged dentin2016

    • Author(s)
      Shinno Y, Ishimoto T, Saito M, Uemura R, Arino M, Marumo K, Nakano T, Hayashi M
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 19849-19849

    • DOI

      10.1038/srep19849

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Multicenter study on caries risk assessment in adults using survival Classification and Regression Trees2016

    • Author(s)
      Arino M, Ito A, Fujiki S, Sugiyama S, Hayashi M
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 29190-29190

    • DOI

      10.1038/srep29190

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] tatistical analysis for subjective and objective evaluations of dental drill sounds2016

    • Author(s)
      Yamada T, Kuwano S, Ebisu S, Hayashi M
    • Journal Title

      PLos One

      Volume: 11 Issue: 7 Pages: e0159926-e0159926

    • DOI

      10.1371/journal.pone.0159926

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Antibacterial activity of resin composites containing surface pre-reacted glass-ionomer (S-PRG) filler2016

    • Author(s)
      Miki S, Kitagawa H, Kitagawa R, Kiba W, Hayashi M, Imazato S
    • Journal Title

      Dental Materials

      Volume: 32 Issue: 9 Pages: 1095-1102

    • DOI

      10.1016/j.dental.2016.06.018

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of a cavity disinfectant containing antibacterial monomer MDPB.2016

    • Author(s)
      Hirose N, Kitagawa R, Kitagawa H, Maezono H, Mine A, Hayashi M, Haapasalo M, Imazato S.
    • Journal Title

      Journal of Dental Research

      Volume: 95 Issue: 13 Pages: 1487-1493

    • DOI

      10.1177/0022034516663465

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Temporal dynamics of bacterial microbiota in the human oral cavity determined using an in situ model of dental biofilms2016

    • Author(s)
      Wake N, Asahi Y, Noiri Y, Hayashi M, Motooka D, Nakamura S, Gotoh K, Miura J, Machi H, Iida T, Ebisu S
    • Journal Title

      npj Biofilms and Microbiome

      Volume: 2 Pages: 16018-16018

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of caries progression in dentin treated by fluoride-containing materials using an in-air micro-PIGE and micro-PIXE measurement system.2015

    • Author(s)
      Yamamoto H*, Iwami Y, Yagi K, Hayashi M, Komatsu H, Okuyama K, Matsuda Y, Yasuda K
    • Journal Title

      Nucl Instrum Meth B

      Volume: 348 Pages: 152-155

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] モダンカリオロジーの世界的潮流を知る2016

    • Author(s)
      林 美加子
    • Organizer
      第23回日本歯科医学会総会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] VA活性リボフラビンによるヒト象牙質の脱灰抑制効果2016

    • Author(s)
      上村怜央、八木香子、新野侑子、松田祐輔、三浦治郎、林 美加子
    • Organizer
      第144回日本歯科保存学会春季学術大会
    • Place of Presentation
      栃木県総合文化センター(栃木県宇都宮市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] n-air micro-beam PIXE/PIGEによる根面象牙質の耐酸性評価-pHおよびフッ化物の影響に関する検討-2016

    • Author(s)
      八木香子、山本洋子、上村怜央、奥山克史、松田康裕、鈴木耕拓、林 美加子
    • Organizer
      第144回日本歯科保存学会春季学術大会
    • Place of Presentation
      栃木県総合文化センター(栃木県宇都宮市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] How best to shift from invasive treatment to preventive care2016

    • Author(s)
      Hayashi M
    • Organizer
      Asia KOL Meetin
    • Place of Presentation
      3M R&D Center (St Paul, USA)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 新規間葉系幹細胞集団による神経再生能の評価2016

    • Author(s)
      伊藤祥作、山内裕香子、成瀬陽菜、伊藤勇紀、林美加子
    • Organizer
      第145回日本歯科保存学会秋季学術大会
    • Place of Presentation
      松本文化会館(長野県松本市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] クロルヘキシジンを配合したセルフアドヒーシブレジンセメントの開発 ‐ 硬化後の抗菌性と長期水中保管後の物性の評価 ‐2016

    • Author(s)
      北川蘭奈、北川晴朗、廣瀬奈々子、三木彩希、和氣菜々子、山口 哲、林 美加子、今里 聡
    • Organizer
      第145回日本歯科保存学会秋季学術大会
    • Place of Presentation
      松本文化会館(長野県松本市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] グラスファイバー配合強化型レジンを用いた間接支台築造法の開発2016

    • Author(s)
      須崎尚子、新野侑子、廣瀬奈々子、エフゲニー コイチェフ、山口哲、今里聡、林 美加子
    • Organizer
      第145回日本歯科保存学会秋季学術大会
    • Place of Presentation
      松本文化会館(長野県松本市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Caries risk assessment in adults using Survival Analysis Classification and Regression Trees.2015

    • Author(s)
      Arino M, Ito A, Fujiki S, Sugiyama S, Hayashi M
    • Organizer
      62nd ORCA 2015
    • Place of Presentation
      Brussels (Belgium)
    • Year and Date
      2015-07-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 活性リボフラビンによるヒト象牙質の強化効果2015

    • Author(s)
      上村怜央、新野侑子、岡本基岐、高橋雄介、林 美加子
    • Organizer
      第142回日本歯科保存学会春季学術大会
    • Place of Presentation
      北九州市・福岡県・北九州国際会議場
    • Year and Date
      2015-06-25
    • Related Report
      2015 Research-status Report
  • [Presentation] In-air micro-beam PIXE/PIGEを用いた根面象牙質の脱灰評価2015

    • Author(s)
      八木香子、山本洋子、岩見行晃、林 美加子
    • Organizer
      第142回日本歯科保存学会春季学術大会
    • Place of Presentation
      北九州市・福岡県・北九州国際会議場
    • Year and Date
      2015-06-25
    • Related Report
      2015 Research-status Report
  • [Presentation] Japan pioneering clinical Guideline treating dental caries.2015

    • Author(s)
      Hayashi M
    • Organizer
      10th International Dental Cokkaboration of the Mekong River Region
    • Place of Presentation
      Bangkok (Thailand)
    • Year and Date
      2015-06-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2015-04-16   Modified: 2022-02-28  

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