UVA-activated riboflavin inhibits demineralization of human dentin
| Project/Area Number |
15K15700
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MINOSHIMA Koji 大阪大学, 大学院工学研究科, 教授 (50174107)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 歯科保存学 / 根面う蝕 / コラーゲン / 紫外線 / リボフラビン / 象牙質 / う蝕 / 架橋 / 保存修復学 / 分子間架橋 |
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| Outline of Final Research Achievements |
Flexural strength is the highest in the group of 0.1% riboflavin exposure and UVA irradiated with 1600 mW/cm2 for 10min, which was significantly higher than control group. Raman spectra showed that a C-C bond in a proline ring was amplified, and the ratio between C-N bond and N-H bond was changed by the riboflavin/UVA treatment. SDS-PAGE analysis suggested that the molecular weight of collagen was increased after the riboflavin/UVA treatment due to new collagen crosslinking.
In μCT analysis, the amount of mineral loss after demineralization in the RF/UVA group was significantly lesser than the control group. In SDS-PAGE analysis, the collagen after the collagenase degradation was less soluble in the riboflavin/UVA group than in the control group because of new collagen crosslinking promoted by a radical reaction.
Riboflavin/UVA treatment was effective in enhancing the acid and enzymatic resistance of human dentin by producing new collagen crosslinking.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Temporal dynamics of bacterial microbiota in the human oral cavity determined using an in situ model of dental biofilms2016
Author(s)
Wake N, Asahi Y, Noiri Y, Hayashi M, Motooka D, Nakamura S, Gotoh K, Miura J, Machi H, Iida T, Ebisu S
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Journal Title
npj Biofilms and Microbiome
Volume: 2
Pages: 16018-16018
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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