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Immunomodulatory effect of human dental pulp cells in autoimmune disease

Research Project

Project/Area Number 15K15724
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Dental engineering/Regenerative dentistry
Research InstitutionAichi Gakuin University

Principal Investigator

Honda Masaki  愛知学院大学, 歯学部, 教授 (70361623)

Co-Investigator(Renkei-kenkyūsha) Watanabe Nobukazu  東京大学, 医科学研究・FACSコアラボラトリー, 特任准教授 (10334278)
Research Collaborator Saito Kouichi  アイル再生医療研究センター
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords歯髄幹細胞 / 自己免疫疾患 / 免疫寛容能 / 制御性T細胞 / ヘルパーT細胞 / リンパ球 / 1型糖尿病 / Th17細胞 / サイトカイン / 細胞傷害性T細胞 / ヒト歯髄幹細胞
Outline of Final Research Achievements

Mesenchymal stromal cells (MSCs) are multipotent progenitors that can be isolated from the connective tissues of most organs. In addition, they exert powerful immunomodulatory effects, including inhibition of proliferation and function of Th17 cells and promotion of Treg differentiation. The objective of this study was to identify immune cell populations that participate in the mechanisms to prevent and reverse type 1 diabetes in the Non-Obese Diabetic (NOD) mouse strain. Co-culture experiments using human dental pulp cells and spleen cells from NOD. These results showed that Treg cells increased the frequency and numbers of interleukin-10 in vitro. The expansion of these cells was a consequence of the proliferation. In contrast, the number of Th17 cells was deceased by co-culture. This study showed that human dental pulp stem cells have a potential to regulate the differentiation into regulatory T cells and Helper T cells.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (40 results)

All 2017 2016 2015 Other

All Journal Article (16 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 16 results,  Open Access: 6 results,  Acknowledgement Compliant: 9 results) Presentation (19 results) (of which Int'l Joint Research: 4 results,  Invited: 7 results) Book (4 results) Remarks (1 results)

  • [Journal Article] <b>Induction of neural crest cells from human dental pulp-derived induced pluripotent stem </b><b>cells </b>2017

    • Author(s)
      Eisuke KAWANO, Taku TORIUMI, Shinya IGUCHI, Daigo SUZUKI, Shuichi SATO, Masaki HONDA
    • Journal Title

      Biomedical Research

      Volume: 38 Issue: 2 Pages: 135-147

    • DOI

      10.2220/biomedres.38.135

    • NAID

      130005606446

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transplantation of mature adipocyte-derived dedifferentiated fat cells into three-wall defects in the rat periodontium induces tissue regeneration2017

    • Author(s)
      Daigo Suzuki, Daisuke Akita, Niina Tsurumachi, Koichiro Kano, Katsuyuki Yamanaka, Tadashi Kaneko, Eisuke Kawano, Shinya Iguchi, Taku Toriumi, Yoshinori Arai, Taro Matsumoto, Shuichi Sato, Masaki Honda
    • Journal Title

      Journal of Oral Science

      Volume: in press

    • NAID

      130006286520

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effect of collagenase concentration for isolating small adipocytes from human buccal fat pad2017

    • Author(s)
      Niina Tsurumachi, Daisuke Akita, Koichiro Kano, Taro Matsumoto, Masaki Hondai
    • Journal Title

      Journal of Oral Science

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effect of local bone marrow stromal cell administration on ligature-induced periodontitis in mice2017

    • Author(s)
      Shinya Iguchi, Daigo Suzuki, Eisuke Kawano, Takayuki Mashimo, Mikihito Kajiya, Taku Toriumi, Toshihisa Kawai, Hidemi Kurihara, Keitaro Isokawa, Shuichi Sato, Masaki Honda
    • Journal Title

      Journal of Oral Sciences

      Volume: in press

    • NAID

      130006286425

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Energy dispersive spectroscopy-scanning transmission electron microscope observations of free radical production in human polymorphonuclear leukocytes phagocytosing non-opsonized Tannerella forsythia2017

    • Author(s)
      Keiichi Moriguchi, Yoshiaki Hasegawa, Naoya Higuchi, Yukitaka Murakami, Fuminobu Yoshimura, Kazuhiko Nakata, Masaki Honda
    • Journal Title

      Microscopy Research Technique

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Use of rat mature adipocyte-derived dedifferentiated fat cells as a cell source for periodontal tissue regeneration.2016

    • Author(s)
      Akita D, Kano K, Saito-Tamura Y, Mashimo T, Sato-Shionome M, Tsurumachi N, Yamanaka K, Kaneko T, Toriumi T, Arai Y, Tsukimura N, Matsumoto T, Ishigami T, Isokawa K, Honda M
    • Journal Title

      Frontier Physiology

      Volume: 7 Pages: 50-50

    • DOI

      10.3389/fphys.2016.00050

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Small buccal fat pad cells have high osteogenic differentiateon potential.2016

    • Author(s)
      Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T, Kazama T, Oki Y, Tamura Y,Tonogi M, Isokawa K, Shimizu N, Honda M
    • Journal Title

      Tissue Engineering Part C: Methods

      Volume: 22 Issue: 3 Pages: 250-9

    • DOI

      10.1089/ten.tec.2015.0420

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 顎関節脱臼防止用ミニプレート開発のための側頭骨鱗部の形態計測2016

    • Author(s)
      木村俊介,大野紀和,本田雅規,小木信美,栗田賢一
    • Journal Title

      愛院大歯誌

      Volume: 54 Pages: 391-398

    • NAID

      40021084046

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 顎関節脱臼防止用ミニプレート開発のためのCTによる側頭骨鱗部の形態計測2016

    • Author(s)
      木村俊介,泉 雅浩,大野紀和,本田雅規,小木信美,栗田賢一
    • Journal Title

      愛院大歯誌

      Volume: 54 Pages: 429-436

    • NAID

      40021084131

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Melatanin enhances vertical bone augmentation in rat calvania secluded spaces.2016

    • Author(s)
      Hiromichi Shino, Akira Hasuike, Yoshinori Arai, Masaki Honda, Keitaro Isokawa, Shuichi Sato.
    • Journal Title

      Medicina Oral Patologia Oral y Cirugia Bucal

      Volume: 21(1)

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Use of Rat Mature Adipocyte-Derived Deffirentiated Fat Cells as a Cell Source for Periodontal Tissue Regeneration.2016

    • Author(s)
      Akita D, Kano K, Saito-Tamura Y, Mashimo T, Sato-Shionome M, Tsurumachi N, Yamanaka K, Kaneko T Toriumi T, Arai Y, Tsukimura N, Matsumoto T, Ishigami T, Iisokawa K, Honda M.
    • Journal Title

      Front Physiol

      Volume: 印刷中

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Small Buccal Fat Pad Cells Have High Osteogenic Differentiation potential.2016

    • Author(s)
      Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T, Kazama T, oki Y, Tamura Y, Tonoji M, Iisokawa K, Shimizu N, Honda M.
    • Journal Title

      Tissue Eng Part C Methods

      Volume: 22(3) Pages: 250-9

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Melatonin enhances vertical bone augmentation in rat calvaria secluded spaces2015

    • Author(s)
      Shino H, Hasuike A, Arai Y, Honda M, Isokawa K, Sato S
    • Journal Title

      Med Oral Patol Oral Cir Bucal

      Volume: 21 Pages: 122-126

    • DOI

      10.4317/medoral.20904

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of mesenchymal progenitor cells in the crown and root pulp of primary teeth.2015

    • Author(s)
      Toriumi T, Takayama N, Murakami M, Sato M, Yuguchi M, Yamazaki Y, Eto K, Otsu M, Nakauchi H, Shirakawa T, Isokawa K, Honda MJ.
    • Journal Title

      Biomed Res

      Volume: 36(1) Pages: 31-45

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Osteogenic cell sheets reinforced with photofunctionalized micro-thin titanium.2015

    • Author(s)
      Ishijima M, Hirota M, Park W, Honda MJ, Tsukimura N, Isokawa K, Ishigami T, Ogawa T
    • Journal Title

      J Biomater

      Volume: 29(10) Pages: 1372-84

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Alkaline phosphatase determines polyphoshate-induced mineralizashon in a cell-type independent manner.2015

    • Author(s)
      Mikami Y, Tsuda H, Akiyama Y, Honda M, Shimizu N, Suzuki N, Komiyma K.
    • Journal Title

      J BoneMiner Metab

      Volume: 印刷中

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 無血清培地にて培養したヒト歯髄細胞の特性と細胞接着の解析2017

    • Author(s)
      大谷憲司,土屋花織,加藤美咲,本田雅規
    • Organizer
      日本再生医療学会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Use of BMSCs prevents alveolar bone resorption on experimental periodontitis2016

    • Author(s)
      Iguchi S, Kawano E, Suzuki D, Tsurumachi N, Kajiya M, Toriumi T, Isokawa K, Arai Y, Fukuda T, Fujisaki Y, Sugano N, Sato S, Honda M
    • Organizer
      The 102nd Annual Meeting of the American Academy of Periodontology in collaboration with the Japanese Society of Periodontology and Japanese academy of Clinical Periodontology
    • Place of Presentation
      San Diego Convention Center (San Diego, CA, USA)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] Dedifferentiated fat cells for tissue engineering in rat periodontal defects2016

    • Author(s)
      Suzuki D, Iguchi S, Kawano E, Tsurumachi N, Toriumi T, Isokawa K, Arai Y, Sakai A, Ooshio K, Sugano N, Sato S, Honda M
    • Organizer
      The 102nd Annual Meeting of the American Academy of Periodontology in collaboration with the Japanese Society of Periodontology and Japanese academy of Clinical Periodontology
    • Place of Presentation
      San Diego Convention Center (San Diego, CA, USA)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒト歯髄細胞から樹立したiPS細胞のエナメル芽細胞,象牙芽細胞,セメント細胞への分化2016

    • Author(s)
      鳥海 拓,河野英輔,岡 篤志,井口慎也,鈴木大悟,磯川桂太郎,本田雅規
    • Organizer
      第25回硬組織再生生物学会学術大会・総会
    • Place of Presentation
      日本大学歯学部(東京都千代田区)
    • Year and Date
      2016-08-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 急性期ラット脊髄圧挫損傷モデルに対する歯髄細胞移植による後肢運動回復への効果2016

    • Author(s)
      大谷憲司,佐久間重光,本田雅規
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 無血清培地にて培養したヒト歯髄細胞の特性の解析2016

    • Author(s)
      加藤美咲,大谷憲司,濱村和紀,本田雅規
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒトiPS細胞から分化誘導させた神経堤細胞の特性2016

    • Author(s)
      鳥海 拓,河野英輔,磯川桂太郎,本田雅規
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒトiPS細胞は歯を形成する細胞に分化できる2016

    • Author(s)
      本田雅規
    • Organizer
      粉体工学会第52回夏期シンポジウム
    • Place of Presentation
      ひょうご共済会館(兵庫県神戸市)
    • Year and Date
      2016-08-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] バイオフィルム感染症治療を目的とした高分子ナノ粒子DDS製剤設計とその評価2016

    • Author(s)
      高橋知里,松原庸博,小川法子,盛口敬一,本田雅規,川嶋嘉明,山本浩充
    • Organizer
      粉体工学会第52回夏期シンポジウム
    • Place of Presentation
      ひょうご共済会館(兵庫県神戸市)
    • Year and Date
      2016-08-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒト歯髄細胞の特性とその細胞治療、そして今後の課題2016

    • Author(s)
      本田雅規
    • Organizer
      第37回日本歯内療法学会学術大会
    • Place of Presentation
      ウインク愛知(愛知県名古屋市)
    • Year and Date
      2016-07-23
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Dental pulp cells as a cell source for tissue regeneration2016

    • Author(s)
      Masaki Honda
    • Organizer
      International Association for Dental Research (IADR) Pulp Biology Regeneration Group (PBGR) Symposium 2016
    • Place of Presentation
      Nagoya Congress Center (Nagoya, Japan)
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Use of human iPS cells for tissue-engineered tooth.2016

    • Author(s)
      Masaki Honda
    • Organizer
      12th International Conference on Tooth Morphogenesis and Differentiation
    • Place of Presentation
      Conference Center (Porvoo, Finland)
    • Year and Date
      2016-06-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] iPS細胞は,エナメル芽細胞,象牙芽細胞,セメント芽細胞に分化する2016

    • Author(s)
      本田雅規,鳥海 拓
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレットふくしま(福島県・郡山市)
    • Year and Date
      2016-03-30
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Human IPS cells ara capable of differentiation into ameloblasts, odontoblasts, and cementoblasts.2016

    • Author(s)
      Honda M. Toriumi T.
    • Organizer
      Gordon Research Conference, Bone and Teeth
    • Place of Presentation
      米国 テキサス州
    • Year and Date
      2016-02-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ラット脊髄圧挫損傷直後の歯髄細胞移植による下肢運動機能への効果.2015

    • Author(s)
      本田雅規,加藤彰子,水谷 誠,内海倫也,盛口敬一
    • Organizer
      第87回愛知学院大学歯学会学術大会
    • Place of Presentation
      愛知学院大学(愛知県・名古屋市)
    • Year and Date
      2015-12-16
    • Related Report
      2015 Research-status Report
  • [Presentation] ラット脊髄圧挫損傷モデルへの歯髄細胞移植の有用性の検討.2015

    • Author(s)
      本田雅規
    • Organizer
      第60回日本口腔外科学会総会・学術大会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-16
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 下歯槽神経切除モデルにおける細胞移植の効果.2015

    • Author(s)
      本田雅規,鳥海 拓,岡 篤志,篠田雅路,岩田幸一,磯川桂太郎.
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ新潟コンベンションセンター(新潟県・新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Tooth-tissue engineering - current status and future directions.2015

    • Author(s)
      Masaki Honda, Taku Toriumi
    • Organizer
      4th Tripartite Conference on Tooth and Bone Deveropment & Regeneration
    • Place of Presentation
      成田ビューホテル(千葉県・成田市)
    • Year and Date
      2015-06-13
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 脱分化脂肪細胞移植による歯周組織再生の試み.2015

    • Author(s)
      本田雅規,秋田大輔,加野浩一郎,鶴町仁奈,鳥海 拓,井口慎也,鈴木大悟,河野英輔,松本太郎,磯川桂一郎.
    • Organizer
      第58回春季日本歯周病学会学術大会
    • Place of Presentation
      幕張メッセ(千葉県・千葉市)
    • Year and Date
      2015-05-16
    • Related Report
      2015 Research-status Report
  • [Book] カラーアトラス 口腔組織発生学2016

    • Author(s)
      磯川桂太郎,稲井哲一郎,入江一元,本田雅規 他13名
    • Total Pages
      135
    • Publisher
      わかば出版
    • Related Report
      2016 Annual Research Report
  • [Book] 言語聴覚士のための基礎知識 臨床歯科医学・口腔外科学2016

    • Author(s)
      新井直也,新崎章,飯野光喜,本田雅規 他多数
    • Total Pages
      311
    • Publisher
      医学書院
    • Related Report
      2016 Annual Research Report
  • [Book] 再生医療・細胞治療のための細胞加工物評価技術2016

    • Author(s)
      大島勇人,本田雅規,佐藤陽治 他多数
    • Total Pages
      239
    • Publisher
      シーエムシー 出版
    • Related Report
      2016 Annual Research Report
  • [Book] Setm cell biology and tissue engineering in dental sciences2015

    • Author(s)
      Masaki J. Honda, Taku Toriumi, Kyoko Oka, Yoko Saito, Keitaro Isokawa
    • Total Pages
      900
    • Publisher
      Elsevier
    • Related Report
      2015 Research-status Report
  • [Remarks] 愛知学院大学歯学部 講座別詳細サイト 口腔解剖学講座

    • URL

      http://www.dent.aichi-gakuin.ac.jp/HP/kokukaibo/index.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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