Development of radio-chemotherapy using lymphogenous drug delivery for lymph node metastasis of oral cancer

• Project/Area Number: 15K15729
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Tohoku University
• Principal Investigator: MIYASHITA HITOSHI 東北大学, 歯学研究科, 助教 (70372323)
• Co-Investigator(Kenkyū-buntansha): 赤松 妃呂子 (須藤妃呂子) 山形大学, 医学部, 医員 (10611981) ; 市川 真由美 山形大学, 医学部, 助教 (20466634) ; 森 士朗 東北大学, 大学病院, 講師 (80230069)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 口腔癌 / リンパ節転移 / リンパ節転移モデル / リンパ節腫脹マウス / 癌化学療法 / 放射線治療 / 腫瘍血管 / リンパ行性癌化学療法 / 転移モデル

Outline of Final Research Achievements

In the present study, development of radio-chemotherapy by using lymphogeneous drug delivery system for oral cancer lymph node metastasis was examined. For this aim, we used a lymph node metastasis mouse model that has lymph nodes of the same size as humans. As a result, since tumor neovasculature was immature in the early stage of metastasis, drugs administrated intravenously were not sufficiently delivered to metastatic lesions. In addition, it was suggested that radiosensitivity of the metastatic lymph nodes tended to decline due to a decrease in oxygen partial pressure in the metastatic lymph nodes with the suppressed blood inflow. As a solution to this problem, when an anticancer agent is injected into a lymph node existing upstream of a metastatic lymph node in the lymph network, it is possible to deliver an anticancer agent to the downstream metastatic lymph node via the lymphatic duct selectively.

Research Products

• [Journal Article] Odontogenic epithelial proliferation is correlated with COX-2 expression in dentigerous cyst and ameloblastoma. (2017)
  • Author(s): Alsaegh MA, Miyashita H, Taniguchi T, Zhu SR
  • Journal Title: Experimental and Therapeutic Medicine Volume: 13 Issue: 1 Pages: 247-253
  • DOI: 10.3892/etm.2016.3939
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Clinical evaluations of autologous fibrin glue and polyglycolic acid sheets as oral surgical wound coverings after partial glossectomy. (2016)
  • Author(s): Kouketsu A, Nogami S, Fujiwara M, Mori S, Yamauchi K, Hashimoto W, Miyashita H, Kurihara J, Kawai T, Higuchi K, Takahashi T.
  • Journal Title: Journal of Craniomaxillofacial Surgery Volume: 44 Issue: 8 Pages: 964-968
  • DOI: 10.1016/j.jcms.2016.05.022
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Odontogenic epithelial proliferation is correlated with COX-2 expression in dentigerous cyst and ameloblastoma (2016)
  • Author(s): Mohammed A. A, H. Miyashita, T. Taniguchi, Sheng R. Zhu
  • Journal Title: Experimental and Therapeutic Medicine Volume: In press
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 当科における口腔細胞診と組織診断の比較検討について (2017)
  • Author(s): 柳沢佑太，宮下 仁，野上晋之介，山内健介，川井 忠，纐纈 衆，及川麻里子， 森 士朗，三浦弘守，熊本裕行，高橋 哲
  • Organizer: 第35回日本口腔腫瘍学会
  • Place of Presentation: 福岡
  • Year and Date: 2017-01-26
• [Presentation] 増大傾向を示した静止性骨空洞に対して内視鏡を用いた口内アプローチにより摘出した脂肪腫の1例 (2017)
  • Author(s): 山内健介，森島浩允，佐藤 工，野上晋之介，宮下 仁，熊本裕行，高橋 哲
  • Organizer: 第35回日本口腔腫瘍学会
  • Place of Presentation: 福岡
  • Year and Date: 2017-01-26
• [Presentation] PIN1 PEPTIDYL PROLYL ISOMERASE INHIBITORS BLOCK CELL PROLIFERATION IN ORAL SQUAMOUS CELL CARCINOMA (2017)
  • Author(s): H. Miyashita, T. Uchida, T. Takahashi
  • Organizer: 43rd Asia-Pacific Advanced Network Dental Session
  • Place of Presentation: 仙台
  • Int'l Joint Research
• [Presentation] Could be detected the precise degree of bone invasion on oral cancer? : Comparison of pathological specimen between preoperative image diagnosis methods. (2016)
  • Author(s): 纐纈 衆，宮下 仁，小嶋 郁穂，阪本 真弥，森 士朗，山内健介，野上晋之介，川井 忠，髙橋 哲
  • Organizer: 第61回日本口腔外科学会
  • Place of Presentation: 千葉
  • Year and Date: 2016-11-25
• [Presentation] Preoperative Evaluation of the Controlling Nutritional Status Score in Patients with cN0 Oral Cancer (2016)
  • Author(s): Kitamura Jun, Miyashita Hitoshi, Hashimoto Wataru, Nogami Shinnosuke, Yamauchi Kensuke, Kataoka Yoshihiro, Abe Satok, Kouketsu Ayumu, Mori Shiro, Shimoda Gen, Ando Fumi, Inamura Naoko, Okamoto Tomoko, Takahashi Tetsu
  • Organizer: 第40回日本頭頸部癌学会
  • Place of Presentation: 大宮
  • Year and Date: 2016-06-09
• [Presentation] 舌扁平上皮癌における原発巣の深さに関する臨床研究 (2016)
  • Author(s): 北村 淳, 野上 晋之介 , 山内 健介, 纐纈 衆, 飯久保 正弘, 古内 壽, 小嶋 郁穂, 宮下 仁, 熊本 裕行, 髙橋 哲
  • Organizer: 第70回日本口腔科学会
  • Place of Presentation: 福岡
  • Year and Date: 2016-04-15
• [Presentation] Emerging concept of oral cancer metastasis induced from analysis of a cancer metastasis model (2015)
  • Author(s): H. Miyashita, S. Mori, T. Kodama, T. Takahashi
  • Organizer: The 22nd International Conference on Oral and Maxillofacial Surgery (ICOMS) 2015
  • Place of Presentation: Melbourne（Australia）
  • Year and Date: 2015-10-27
  • Int'l Joint Research