Analysis and establishment of glossodynia model mouse from the view of the pain memory malformation hypothesis in hippocampal area

• Project/Area Number: 15K15730
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: University of Tsukuba
• Principal Investigator: YANAGAWA Toru 筑波大学, 医学医療系, 准教授 (10312852)
• Co-Investigator(Kenkyū-buntansha): 田渕 克彦 信州大学, 学術研究院医学系, 教授 (20546767)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 舌痛症 / neuroligin / 急性海馬スライス

Outline of Final Research Achievements

We hypothesed that glossodynia was caused by memory formation abnormality, and performed following experiments and obtained results. 1）CFA was injected into mouse and checked the IDO-1 expression in hippocampal area by immunostaining but there seemed to be no relation between pain memory and IDO-1 expression. 2) β actin expression in the 2/3 layer of the somatic sensory area by genome editing of CRISPR/Cas9 and the in utero electroporation was confirmed. 3) Difference of the neuronal activity with the patch clamp was observed in hipocampal slice of knock in mouse (R704C) and in the dispersion neuroligin2 knockdown nerve culture. These results show the possibility that the pain threshold was changed in the model mouse.

Research Products

• [Journal Article] Distortion of the normal function of synaptic cell adhesion molecules by genetic variants as a risk for autism spectrum disorders. (2017)
  • Author(s): Baig DN, Yanagawa T, Tabuchi K.
  • Journal Title: Brain Res Bull. Volume: 129 Pages: 82-90
  • DOI: 10.1016/j.brainresbull.2016.10.006
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques. (2016)
  • Author(s): Uemura T, Mori T, Kurihara T, Kawase S, Koike R, Satoga M, Cao X, Li X, Yanagawa T, Sakurai T, Shindo T, Tabuchi K.
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 35861-35861
  • DOI: 10.1038/srep35861
  • NAID: 120007100133
  • Peer Reviewed / Open Access / Acknowledgement Compliant