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Analysis and establishment of glossodynia model mouse from the view of the pain memory malformation hypothesis in hippocampal area

Research Project

Project/Area Number 15K15730
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

YANAGAWA Toru  筑波大学, 医学医療系, 准教授 (10312852)

Co-Investigator(Kenkyū-buntansha) 田渕 克彦  信州大学, 学術研究院医学系, 教授 (20546767)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords舌痛症 / neuroligin / 急性海馬スライス
Outline of Final Research Achievements

We hypothesed that glossodynia was caused by memory formation abnormality, and performed following experiments and obtained results. 1)CFA was injected into mouse and checked the IDO-1 expression in hippocampal area by immunostaining but there seemed to be no relation between pain memory and IDO-1 expression. 2) β actin expression in the 2/3 layer of the somatic sensory area by genome editing of CRISPR/Cas9 and the in utero electroporation was confirmed. 3) Difference of the neuronal activity with the patch clamp was observed in hipocampal slice of knock in mouse (R704C) and in the dispersion neuroligin2 knockdown nerve culture. These results show the possibility that the pain threshold was changed in the model mouse.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2017 2016

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results)

  • [Journal Article] Distortion of the normal function of synaptic cell adhesion molecules by genetic variants as a risk for autism spectrum disorders.2017

    • Author(s)
      Baig DN, Yanagawa T, Tabuchi K.
    • Journal Title

      Brain Res Bull.

      Volume: 129 Pages: 82-90

    • DOI

      10.1016/j.brainresbull.2016.10.006

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques.2016

    • Author(s)
      Uemura T, Mori T, Kurihara T, Kawase S, Koike R, Satoga M, Cao X, Li X, Yanagawa T, Sakurai T, Shindo T, Tabuchi K.
    • Journal Title

      Sci Rep

      Volume: 6 Issue: 1 Pages: 35861-35861

    • DOI

      10.1038/srep35861

    • NAID

      120007100133

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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