Elucidation of fibrous dysplasia pathogenesis using disease-specific iPS cells and application to drug discovery
| Project/Area Number |
15K15733
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HOSHI Kazuto 東京大学, 医学部附属病院, 准教授 (30344451)
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| Co-Investigator(Kenkyū-buntansha) |
高戸 毅 東京大学, 医学部附属病院, 教授 (90171454)
西澤 悟 東京大学, 医学部附属病院, 特任助教 (00646200)
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| Co-Investigator(Renkei-kenkyūsha) |
OTSU Makoto 東京大学, 医科学研究所, 准教授 (30361330)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 線維性骨異形成症 / 疾患特異的iPS細胞 / ゲノム編集 / 創薬 / 創薬スクリーニング |
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| Outline of Final Research Achievements |
Fibrotic dysplasia is a disease in which normal bones replace with young bones and connective tissues, resulting in bone deformity and pathological fractures as the main symptoms. Mutation of the GNAS1 gene is known as one of the causes of onset. Currently, the only treatment method for the disease is bone loss surgery, and no fundamental therapeutic agent has been developed yet.
To elucidate the pathophysiology of fibrotic dysplasia and discover therapeutic drugs, a large amount of lesion cells of the disease are required. Therefore, we incorporated GNAS1 mutant genes into iPS cells by genome editing technology to obtain GNAS1 mutant iPS cells, which could be a tool for producing large amounts of diseased cells. In addition, we constructed a screening system to investigate compounds effective for the treatment of this disease.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] T2 and apparent diffusion coefficient of MRI reflect maturation of tissue-engineered auricular cartilage subcutaneously transplanted in rats2016
Author(s)
Fujihara Y, NItta N, Misawa M, Hyodo K, Shirasaki Y, Hayashi K, Kosaka R, Homma K, Numano T, Kuribayashi S, Watanabe Y, Sato J, Ohtomo K, Takato T, Hoshi K
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Journal Title
Tissue Eng Part C Methods
Volume: 22(5)
Issue: 5
Pages: 429-438
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Production of three-dimensional tissue-engineered cartilage through mutual fusion of chondrocyte pellets.2016
Author(s)
Hoshi K, Fujihara Y, Mori Y, Asawa Y, Kanazawa S, Nishizawa S, Misawa M, Numano T, Inoue H, Sakamoto T, Watanabe M, Komura M, Takato T.
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Journal Title
Int J Oral Maxillofac Surg.
Volume: Sep; 45(9)
Issue: 9
Pages: 1177-1185
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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