Explore for master regulator of EMT in oral cancer

• Project/Area Number: 15K15740
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: TSUNEMATSU Takaaki 徳島大学, 大学院医歯薬学研究部(医学系), 助教 (70726752)
• Co-Investigator(Kenkyū-buntansha): 工藤 保誠 徳島大学, 大学院医歯薬学研究部(歯学系), 准教授 (50314753)
• Co-Investigator(Renkei-kenkyūsha): ISHIMARU Naozumi 徳島大学, 大学院医歯薬学研究部, 教授 (60314879) ; ARAKAKI Rieko 徳島大学, 大学院医歯薬学研究部, 准教授 (00193061)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口腔扁平上皮癌 / EMT / 口腔癌 / 上皮間葉移行

Outline of Final Research Achievements

It is known that Epithelial Mesenchymal Transition, EMT is important step in cancer invasion and metastasis. Interestingly, there are spindle cell carcinoma with EMT phenotype known as the subtype of oral squamous cell carcinoma . In this study, we tried to explore the master regulator of EMT by using spindle cell carcinoma cell lines which we previously established. We could not identify the master regulator of EMT but established the spindle cell carcinoma cell line with reporter gene. In future, we try to identify the master regulator of EMT by using this reporter cell line.

Research Products

• [Journal Article] Clear cell odontogenic carcinoma: establishment and characterization of a clear cell odontogenic carcinoma cell line with EWSR1-ATF1 fusion gene. (2017)
  • Author(s): Kujiraoka S, Tsunematsu T, Sato Y, Yoshida M, Ishikawa A, Tohyama R, Tanaka M, Kobayashi Y, Kondo T, Ushio A, Otsuka K, Kurosawa M, Saito M, Yamada A, Arakaki R, Nagai H, Nikai H, Takeuchi K, Nagao T, Miyamoto Y, Ishimaru N, Kudo Y.
  • Journal Title: Oral Oncology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Primary Biliary Cholangitis: Its Pathological Characteristics and Immunopathological Mechanisms (2017)
  • Author(s): Tsuneyama K, Baba H, Morimoto Y, Tsunematsu T, Ogawa H.
  • Journal Title: The Journal of Medical Investigation Volume: 64 Issue: 1.2 Pages: 7-13
  • DOI: 10.2152/jmi.64.7
  • NAID: 130005501980
  • ISSN: 1343-1420, 1349-6867
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Daily Coffee Intake Inhibits Pancreatic Beta Cell Damage and Nonalcoholic Steatohepatitis in a Mouse Model of Spontaneous Metabolic Syndrome, Tsumura-Suzuki Obese Diabetic Mice. (2017)
  • Author(s): Watanabe S, Takahashi T, Ogawa H, Uehara H, Tsunematsu T, Baba H, Morimoto Y, Tsuneyama K.
  • Journal Title: Metab Syndr Relat Disord. Volume: 15(4) Issue: 4 Pages: 170-177
  • DOI: 10.1089/met.2016.0114
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ameloblastin induces tumor suppressive phenotype and enhances chemosensitivity to doxorubicin via Src-Stat3 inactivation in osteosarcoma. (2016)
  • Author(s): Ando T, Kudo Y, Iizuka S, Tsunematsu T, Umehara H, Shrestha M, Matsuo T, Kubo T, Shimose S, Arihiro K, Ogawa I, Ochi M, Takata T.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 40187-40187
  • DOI: 10.1038/srep40187
  • NAID: 120006534974
  • Peer Reviewed / Open Access
• [Journal Article] The Nuclear Receptor AhR Controls Bone Homeostasis by Regulating Osteoclast Differentiation via the RANK/c-Fos Signaling Axis. (2016)
  • Author(s): Izawa T, Arakaki R, Mori H, Tsunematsu T, Kudo Y, Tanaka E, Ishimaru N.
  • Journal Title: The Journal of Immunology Volume: 197(12) Pages: 4639-4650
• [Journal Article] Oral environment and cancer. (2016)
  • Author(s): Kudo Y, Tada H, Fujiwara N, Tada Y, Tsunematsu T, Miyake Y, Ishimaru N.
  • Journal Title: Genes Environ. Volume: 1 Issue: 1 Pages: 38-13
  • DOI: 10.1186/s41021-016-0042-z
  • NAID: 120006937648
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Human odontogenic epithelial cells derived from epithelial rests of Malassez possess stem cell properties. (2016)
  • Author(s): Tsunematsu T, Fujiwara N, Yoshida M, Takayama Y, Kujiraoka S, Qi G, Kitagawa M, Kondo T, Yamada A, Arakaki R, Miyauchi M, Ogawa I, Abiko Y, Nikawa H, Murakami S, Takata T, Ishimaru N, Kudo Y.
  • Journal Title: Lab Invest. Volume: 96(10) Issue: 10 Pages: 1063-1075
  • DOI: 10.1038/labinvest.2016.85
  • NAID: 120006718349
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] PARP6 acts as a tumor suppressor via dowregulating Survivin expression in colorectal cancer. (2016)
  • Author(s): Qi G, Kudo Y, Tang B, Liu T, Jin S, Liu J, Zuo X, Mi S, Shao W, Ma X, Tsunematsu T, Ishimaru N, Zeng S, Tatsuka M, Shimamoto F.
  • Journal Title: Oncotarget. Volume: 印刷中 Issue: 14 Pages: 18812-18824
  • DOI: 10.18632/oncotarget.7712
  • Peer Reviewed
• [Journal Article] MicroRNA-203 Suppresses Invasion and Epithelial-mesenchymal Transition Induction via Targeting NUAK1 and SNAI2 in Head and Neck Cancer (2016)
  • Author(s): Obayashi, M., Yoshida, M., Tsunematsu, T., Ogawa, I., Sasahira, T., Kuniyasu, H., Imoto, I., Abiko, Y., Xu, D., Fukunaga, S., Tahara, H., Kudo, Y., Nagao, T., Takata, T.
  • Journal Title: Oncotarget Volume: 6972 Issue: 7 Pages: 8223-39
  • DOI: 10.18632/oncotarget.6972
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Acceleration of tumor growth due to dysfunction in Ma macrophages and enhanced angiogenesis in an animal model of autoimmune disease. (2016)
  • Author(s): Kondo T, Tsunematsu T, Yamada A, Arakaki R, Saito M, Otsuka K, Kujiraoka S, Ushio A, Kurosawa M, Kudo Y, Ishimaru N.
  • Journal Title: Lab Invest Volume: 96 Issue: 4 Pages: 468-480
  • DOI: 10.1038/labinvest.2015.166
  • Peer Reviewed / Open Access
• [Journal Article] Role of regulatory T cell in inflammatory bowel disease. (2016)
  • Author(s): Yamada A, Arakaki R, Tsunematsu T, Kudo Y, Ishimaru N.
  • Journal Title: World J Gastroenterol. Volume: 22 Issue: 7 Pages: 2195-2205
  • DOI: 10.3748/wjg.v22.i7.2195
  • Peer Reviewed / Open Access
• [Journal Article] Tumor-promoting function and prognostic significance of the RNA-binding protein T-cell intracellular antigen-1 in esophageal squamous cell carcinoma (2015)
  • Author(s): Hamada J, Shoda K, Masuda K, Fujita Y, Naruto T, Kohmoto T, Miyakami Y, Watanabe M, Kudo Y, Fujiwara H, Ichikawa D, Otsuji E, Imoto I
  • Journal Title: Oncotarget Volume: - Issue: 13 Pages: 17111-17128
  • DOI: 10.18632/oncotarget.7937
  • NAID: 120006240043
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Impaired Expansion of Regulatory T Cells in A Neonatal Thymectomy-Induced Autoimmune Mouse Model. (2015)
  • Author(s): Yamada A, Ushio A, Arakaki R, Tsunematsu T, Kudo Y, Hayashi Y, Ishimaru N.
  • Journal Title: Am J Pathol Volume: 185 Issue: 11 Pages: 2886-2897
  • DOI: 10.1016/j.ajpath.2015.07.007
  • NAID: 120006711357
  • Peer Reviewed / Open Access
• [Journal Article] The non-canonical role of Aurora-A in DNA replication. Front Oncol (2015)
  • Author(s): Tsunematsu T, Arakaki R, Yamada A, Ishimaru N, Kudo Y
  • Journal Title: Front Oncol Volume: 96 Pages: 468-480
  • DOI: 10.3389/fonc.2015.00187
  • Peer Reviewed / Open Access
• [Journal Article] A Critical Role for Thymic Stromal Lymphopoietin in Nickel-Induced Allergy in Mice. (2014)
  • Author(s): Ashrin MN, Arakaki R, Yamada A, Kondo T, Kurosawa M, Kudo Y, Watanabe M, Ichikawa T, Hayashi Y, Ishimaru N.
  • Journal Title: J Immunol. Volume: 192 Issue: 9 Pages: 4025-4031
  • DOI: 10.4049/jimmunol.1300276
  • Peer Reviewed / Open Access
• [Presentation] タンパク質のユビキチン化を介した細胞周期と分化の制御機構の解明 (2017)
  • Author(s): 常松貴明
  • Organizer: 第2回口腔医科学フロンティア研究会
  • Place of Presentation: ＺＡＯセンタープラザ「蔵王・山形」
  • Year and Date: 2017-03-05
  • Invited
• [Presentation] 癌の発生・進展に関する分子病理学的研究 (2016)
  • Author(s): 常松貴明
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター「宮城・仙台」
  • Year and Date: 2016-05-12
  • Invited
• [Presentation] 染色体パッセンジャー複合体タンパク質Borealinのユビキチン分解の意義とその癌化への関与 (2016)
  • Author(s): 常松貴明、工藤保誠、山田安希子、新垣理恵子、小川博久、常山幸一、石丸直澄
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター「宮城・仙台」
  • Year and Date: 2016-05-12
• [Presentation] Chromosome Passenger Complex構成因子Borealinの細胞周期依存的 なタンパク分解とその癌化への関与 (2015)
  • Author(s): 常松貴明, 工藤保誠, 近藤智之, 大塚邦紘, 牛尾綾, 鯨岡聡子, 斎藤雅子, 山田安希子, 新垣理恵子, 石丸直澄
  • Organizer: 第26回日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: 北海道大学学術交流会館(北海道札幌市)
  • Year and Date: 2015-07-31
• [Presentation] 染色体パッセンジャー複合体タンパク質Borealinのユビキチン分解とその癌化への関与 (2015)
  • Author(s): 常松貴明, 工藤保誠, 近藤智之, 牛尾綾, 鯨岡聡子, 山田安希子, 新垣理恵子, 石丸直澄
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋国際会議場(愛知県名古屋市)
  • Year and Date: 2015-05-02