3D analysis of the oral mucosa epithelium barrier function

• Project/Area Number: 15K15747
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Keio University
• Principal Investigator: KATO SHIN 慶應義塾大学, 医学部(信濃町), 助教 (80383719)
• Co-Investigator(Kenkyū-buntansha): 角田 和之 慶應義塾大学, 医学部(信濃町), 講師 (60265915)
• Co-Investigator(Renkei-kenkyūsha): SATO Hidekazu
• Research Collaborator: AMAGAI Masayuki ; KUBO Ryouji ; FUJITA KOHEI
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: タイトジャンクション / 口腔粘膜 / 三次元的観察 / 共焦点顕微鏡 / 三次元構造

Outline of Final Research Achievements

On the basis of the reported cofocus microscope and observation method of the tight junction in the mouse auricle epithelium using the 3D construction software, I examined a condition such as one of reagent density so far at dyeing time to establish the observation method of the tight junction suitable for an oral mucosa epithelium more. As a result, observation of ZO-1 which was Claudin-1 which was the constitution protein of the tight junction of the mouse oral mucosa than a conventional method more clearly, Claudin-4 and lining protein was enabled by increasing primary dyeing time.

Research Products

• [Journal Article] A titanium screw-retaining temporary denture on the raw surface muscular flap for the immediate maxillary reconstruction. (2016)
  • Author(s): Miyashita H, Asoda S, Horie N, Usuda S, Kato S, Muraoka W, Yamada Y, Yoshikawa M, Hasegawa T, Onizawa K, Tanaka I, Yazawa M, Ogata H, Kishi K, Tomita T, Nakagawa, Kawana H
  • Journal Title: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology Volume: 28 Pages: 8-12
  • Peer Reviewed
• [Journal Article] 上顎に発生し嚢胞状を呈した石灰化上皮性歯原性腫瘍の1例. (2015)
  • Author(s): 軽部健史, 加藤伸, 高森康次, 長谷川稔洋, 池内忍, 河奈裕正
  • Journal Title: 日本口腔外科学会雑誌 Volume: 61 Pages: 246-250
  • NAID: 130005102755
  • Peer Reviewed
• [Presentation] 皮膚水疱形成を伴なった粘膜優位型尋常性天疱瘡の1例. (2015)
  • Author(s): 潮田裕梨, 加藤伸, 角田和之, 舟山一成, 中川種昭, 河奈裕正
  • Organizer: 第200回日本口腔外科学会関東支部学術集会
  • Place of Presentation: 東京大学鉄門記念講堂（東京都文京区）
  • Year and Date: 2015-12-19
• [Presentation] H. Shiba, S. Kato, T. Nakagawa. Use of navigation systems in orthognathic surgery. (2015)
  • Author(s): Use of navigation systems in orthognathic surgery.
  • Organizer: International Conference on Oral and Maxillofacial Surgery
  • Place of Presentation: メルボルン（オーストラリア）
  • Year and Date: 2015-10-27
  • Int'l Joint Research
• [Presentation] 当科を受診した腫瘍随伴性天疱瘡症例の臨床的検討. (2015)
  • Author(s): 藤田康平, 佐藤英和, 加藤伸, 西須大徳, 池浦一裕, 潮田裕梨, 石井秀太郎, 角田博之, 天谷雅行, 中川種昭, 角田和之
  • Organizer: 第25回日本口腔内科学会学術集会
  • Place of Presentation: 大阪大学コンベンションセンター（大阪府吹田市）
  • Year and Date: 2015-09-18
• [Presentation] 顎枝矢状分割術専用吸収性骨接合材料（Super FIXSORB-MX X型4穴）の使用経験. (2015)
  • Author(s): 井上真理子, 加藤伸, 福田仁, 椎葉勇介, 中川種昭, 柴秀行
  • Organizer: 第25回日本顎変形症学会総会
  • Place of Presentation: ベルサール神田（東京都千代田区）
  • Year and Date: 2015-06-04
• [Presentation] 手術用ナビゲーションシステムを用いた下顎枝矢状分割術時の近位骨片位置決定方法. (2015)
  • Author(s): 加藤伸, 井上真理子, 椎葉勇介, 福田仁, 中川種昭, 柴秀行
  • Organizer: 第25回日本顎変形症学会総会
  • Place of Presentation: ベルサール神田（東京都千代田区）
  • Year and Date: 2015-06-04