| Project/Area Number |
15K15747
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Keio University |
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Principal Investigator |
KATO SHIN 慶應義塾大学, 医学部(信濃町), 助教 (80383719)
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| Co-Investigator(Kenkyū-buntansha) |
角田 和之 慶應義塾大学, 医学部(信濃町), 講師 (60265915)
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Hidekazu
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| Research Collaborator |
AMAGAI Masayuki
KUBO Ryouji
FUJITA KOHEI
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | タイトジャンクション / 口腔粘膜 / 三次元的観察 / 共焦点顕微鏡 / 三次元構造 |
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| Outline of Final Research Achievements |
On the basis of the reported cofocus microscope and observation method of the tight junction in the mouse auricle epithelium using the 3D construction software, I examined a condition such as one of reagent density so far at dyeing time to establish the observation method of the tight junction suitable for an oral mucosa epithelium more. As a result, observation of ZO-1 which was Claudin-1 which was the constitution protein of the tight junction of the mouse oral mucosa than a conventional method more clearly, Claudin-4 and lining protein was enabled by increasing primary dyeing time.
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