| Project/Area Number |
15K15749
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka Medical College |
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Principal Investigator |
Hirata Azumi 大阪医科大学, 医学部, 准教授 (40263587)
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| Co-Investigator(Kenkyū-buntansha) |
野村 良太 大阪大学, 歯学研究科, 准教授 (90437385)
高野 和文 京都府立大学, 生命環境科学研究科, 教授 (40346185)
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| Research Collaborator |
GOLDBERG Michel Université Paris Descartes, INSERM UMR-S 1124・Biomédicale des Saints Pères, Professeur Emerite
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | コラーゲン結合タンパク質 / Streptococcus mutans / 構造解析 / 機能解析 / S. mutans / コラーゲン / 病原性 / 結晶構造 |
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| Outline of Final Research Achievements |
Collagen-binding-proteins of Streptococcus mutans, Cnm/Cbm, have been reported to be as risk factors associated with bacteremia, cardiovascular diseases, and hemorrhagic stroke. In this study, the crystal structure of collagen-binding-domain of Cnm, Cnm30-325, was determined by molecular replacement methods, using the Cna31-344 crystal structure as a search model. The structure of collagen-binding-domain of Cbm, Cbm31-330, was modeled by SWISS-MODEL using the structure of Cnm30-325. The crystal structure of Cnm30-325 exhibits two distinct domains called N1 and N2. The structure of Cbm31-330 is very similar to the structure of Cnm30-325. N1, N2 and Linker form the hole and multiple sites in this area are related to bind to collagen, suggesting that it may be collagen-binding site.
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| Academic Significance and Societal Importance of the Research Achievements |
Cnm/Cbmにおいて、本研究により明らかとなったコラーゲンとの相互作用に関わる領域は、既知のコラーゲン結合タンパク質Cnaの領域と共通しており、この部位はコラーゲン結合タンパク質においてコラーゲンとの結合に関わる最も重要な部位であることが示唆された。
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