Research Project
Grant-in-Aid for Challenging Exploratory Research
To select the optimal therapeutic strategy for individual patients with cancer, clarification of the biological behaviorof the tumor using minimal invasive methods is necessary. Circulating tumor cells (CTCs) and circulating cell-free DNA (ctDNA) released into the bloodstream from the primary tumor and metastases reflect the current tumor status. To establish a noninvasive method for identifying mutation status in tumor cells, we tried to develop methods for next-generation sequencing (NGS) from CTCs and ctDNA. CTCs were isolated using a cascading spiral system with patient blood (Hou et al, Scientific Rep. 2013) while ctDNA and DNA extracted from CTCs were analyzed by NGS (Ion PGM; LifeTechnology). Thirty-four patients with gastric, colorectal, esophageal, or head and neck cancers were enrolled in this study. A total of 8-77 CTCs/ml were retrieved from whole blood. Mutation profiles were successfully identified from CTCs and ctDNA obtained from each patient by NGS.
All 2016 2015
All Journal Article (4 results) (of which Int'l Joint Research: 3 results, Peer Reviewed: 4 results, Open Access: 4 results, Acknowledgement Compliant: 3 results) Presentation (4 results) (of which Int'l Joint Research: 4 results, Invited: 1 results)
Oncotarget
Volume: Apr 21 Issue: 22 Pages: 1-14
10.18632/oncotarget.8890
BMC Cancer
Volume: 16 Issue: 1 Pages: 192-195
10.1186/s12885-016-2229-x
Scientific Reports
Volume: Nov 9;5 Issue: 1 Pages: 15921-15921
10.1038/srep15921
Cell Biosci.
Volume: Aug 18;5 Issue: 1 Pages: 41-49
10.1186/s13578-015-0031-0
