Effects of chewing during prenatal stress on diabetes onset in mouse offspring

• Project/Area Number: 15K15761
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Nagoya Women's University (2017); Seijoh University (2015-2016)
• Principal Investigator: Kubo Kin-ya 名古屋女子大学, 家政学部, 教授 (00329492)
• Co-Investigator(Kenkyū-buntansha): 笹栗 健一 自治医科大学, 医学部, 講師 (10235286) ; 東 華岳 産業医科大学, 医学部, 教授 (20273146) ; 飯沼 光生 朝日大学, 歯学部, 教授 (70184364) ; 小野塚 実 神奈川歯科大学, 歯学部, 名誉教授 (90084780)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: prenatal stress / chewing / diabetes mellitus / adiposeness / fat / insullin resistance / 咀嚼運動 / 妊娠ストレス / 糖尿病 / 肥満 / ストレスコーピング

Outline of Final Research Achievements

Maternal stress induces decreased birth weights and glucose intolerance, leading to diabetes in the pups. We examined the effects of chewing during maternal restraint stress on changes in weight, blood glucose levels, and energy metabolism-related materials in the pup. Pregnant mice were exposed to restraint stress. Half of dams were given a wooden stick to chew during restraint stress. Restraint stress during pregnancy increased body weight, amount of food ingested, blood glucose levels, insulin resistance, plasma leptin levels and NPY mRNA expression, and decreased blood adiponectin levels in the adult pups. Adult offspring of dams allowed to chew during restraint stress exhibited decreased in body weight, amount of food ingested, blood glucose levels, insulin resistance, plasma leptin levels and NPY mRNA expression, and increased blood adiponectin levels. These findings suggest that maternal chewing ameliorates maternal stress-induced diabetes in the adult offspring.

Research Products

• [Journal Article] Maternal Active Mastication during Prenatal Stress Ameliorates Prenatal Stress-Induced Lower Bone Mass in Adult Mouse Offspring (2017)
  • Author(s): Azuma K, Ogura M, Kondo H, Suzuki A, Hayashi S, Iinuma M, Onozuka M, Kubo KY
  • Journal Title: Int J Med Sci Volume: 14 Issue: 4 Pages: 348-355
  • DOI: 10.7150/ijms.18298
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Association between mastication, the hippocampus, and the HPA axis: a comprehensive review. (2017)
  • Author(s): Azuma K, Zhou Q, Niwa M, Kubo KY
  • Journal Title: Int J Mol Sci Volume: 18 Issue: 8 Pages: 1867-1867
  • DOI: 10.3390/ijms18081687
  • Peer Reviewed / Open Access
• [Journal Article] Maternal chewing during prenatal stress ameliorates stress-induced hypomyelination, synaptic alterations, and learning impairment in mouse offspring (2016)
  • Author(s): Suzuki A, Iinuma M, Hayashi S, Sato Y, Azuma K, Kubo KY
  • Journal Title: Brain Res Volume: 91 Pages: 36-43
  • DOI: 10.1016/j.brainres.2016.09.007
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant