| Project/Area Number |
15K15763
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 小胞体ストレス応答 / 歯周組織破壊 / 歯周炎 / 小胞体ストレス / ERAIマウス / 分子イメージング / ERストレス |
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| Outline of Final Research Achievements |
Endoplasmic reticulum (ER) stress is the cell response that activates the unfolded protein response (UPR) pathway in a variety of conditions, such as inflammation and bone metabolism.In order to clarify the exact location of the UPR response in periodontal tissues in periodontal disease, we induced periodontal tissue destraution by administering Porphyromonas gingivalis, a periodontopathic bacterium, and ligature placement around the upper second molar in ERAI mouse in which UPR reponse can be visualized. Although the upregulation of the UPR reponse-related gene was observed by using conventional method, this reponse cannot be visualized in ERAI mouse. We tried to detect UPR response visually in gingival tissue by administering tunicamysin, a UPR response-inducing agent or pancreas, consistently occurring UPR response as positive control experiments. However, we wereunable to detect UPR response visually.
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