Synthesis of artificial genome consisting of only proteins used by early life
| Project/Area Number |
15K16083
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Life / Health / Medical informatics
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Amikura Kazuaki 東京大学, 大学院新領域創成科学研究科, 助教 (60735918)
|
|---|
| Research Collaborator |
Kiga Daisuke 早稲田大学, 先進理工学部・電気・情報生命工学科, 教授 (30376587)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 合成生物学 / 無細胞翻訳系 / リボソーム / tRNA / リポソーム / 構成的アプローチ / 試験管内再構成 / タンパク質 / ゲノム / 無細胞 |
|---|
| Outline of Final Research Achievements |
This study is a research contributing to the development of basic technologies for artificial genome construction. In this study, we developed a system which exhibits desired function by cooperation of multiple kinds of proteins in cell-free translation system. In particular, we were focused on ribosome-related genes and other genes. The in vitro reconstitution system of ribosome using recombinant protein was able to construct a system with higher reconstitution efifciency of ribosome by using biosynthesis factor. In addition, new findings on the function of each factor in the ribosome biosynthesis process were presented.
|
Report
(4 results)
Research Products
(27 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Single-molecule studies of ribosome assembly: Coupling transcription and assembly2016
Author(s)
Ueda, T., Aoyama, R., Amikura, K., Bercy, M., Bizebard, T., Bockelmann, U., Biebricher, A., Wuite, G.J.L., Peterman, E.J.G., Nikolay, R., Hilal, T., Bo, Q., and Nierhaus, K.H.
Organizer
the 16th HFSP Awardees Meeting
Place of Presentation
Biopolice, Singapore
Year and Date
2016-07-10
Related Report
Int'l Joint Research
-
[Presentation] Single-molecule studies of ribosome assembly: Coupling transcription and assembly2016
Author(s)
Ueda, T., Aoyama, R., Amikura, K., Bercy, M., Bizebard, T., Bockelmann, U., Biebricher, A., Wuite, G.J.L., Peterman, E.J.G., Nikolay, R., Hilal, T., Bo, Q., and Nierhaus, K.H.
Organizer
the 16th HFSP Awardees Meeting
Place of Presentation
Singapore
Year and Date
2016-07-10
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
