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Synthesis of artificial genome consisting of only proteins used by early life

Research Project

Project/Area Number 15K16083
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Life / Health / Medical informatics
Research InstitutionThe University of Tokyo

Principal Investigator

Amikura Kazuaki  東京大学, 大学院新領域創成科学研究科, 助教 (60735918)

Research Collaborator Kiga Daisuke  早稲田大学, 先進理工学部・電気・情報生命工学科, 教授 (30376587)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords合成生物学 / 無細胞翻訳系 / リボソーム / tRNA / リポソーム / 構成的アプローチ / 試験管内再構成 / タンパク質 / ゲノム / 無細胞
Outline of Final Research Achievements

This study is a research contributing to the development of basic technologies for artificial genome construction. In this study, we developed a system which exhibits desired function by cooperation of multiple kinds of proteins in cell-free translation system. In particular, we were focused on ribosome-related genes and other genes. The in vitro reconstitution system of ribosome using recombinant protein was able to construct a system with higher reconstitution efifciency of ribosome by using biosynthesis factor. In addition, new findings on the function of each factor in the ribosome biosynthesis process were presented.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (27 results)

All 2018 2017 2016 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (22 results) (of which Int'l Joint Research: 6 results,  Invited: 4 results) Book (2 results) Remarks (1 results)

  • [Journal Article] De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles2018

    • Author(s)
      Furusato Takumi、Horie Fumihiro、Matsubayashi Hideaki T.、Amikura Kazuaki、Kuruma Yutetsu、Ueda Takuya
    • Journal Title

      ACS Synthetic Biology

      Volume: 7 Issue: 4 Pages: 953-961

    • DOI

      10.1021/acssynbio.7b00350

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cell-free translation system: Development in biochemistry and advance in synthetic biology2017

    • Author(s)
      金森 崇,杉本(永池) 崇,車 兪澈,網藏 和晃,上田 卓也
    • Journal Title

      Journal of Japanese Biochemical Society

      Volume: 89 Pages: 211-220

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 再生産可能な再構成型無細胞翻訳系2018

    • Author(s)
      網藏和晃, 青山遼, 日比敬太, 下條優, 風穴彰洋, 福嶋瑞穂, 清水義宏, 上田卓也
    • Organizer
      生命の起原および進化学会, 第 43 回学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 全ての生命が普遍的に有する翻訳系の起源2017

    • Author(s)
      網蔵和晃
    • Organizer
      第5回宇宙における生命ワークショップ
    • Place of Presentation
      一橋講堂(東京、千代田区)
    • Year and Date
      2017-03-06
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 試験管内転写された tRNAによるタンパク質合成系の構築2017

    • Author(s)
      日比 敬太、杉浦 直樹、網藏 和晃、清水 義宏、横川 隆志、上田 卓也
    • Organizer
      第12回無細胞生命科学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] リボソームの試験管内再構成系から考える生命進化2017

    • Author(s)
      網藏 和晃
    • Organizer
      日本進化学会弟19回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] リポソーム内での大腸菌細胞骨格因子群のde novo合成2017

    • Author(s)
      古里 匠、堀江 史博、松林 英明、○網藏 和晃、車 ゆうてつ、上田 卓也
    • Organizer
      第14回21世紀大腸菌研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 大腸菌50Sリボソームサブユニット初期前駆体の試験管内再構成2017

    • Author(s)
      青山 遼, 網藏 和晃, 上田 卓也
    • Organizer
      第14回21世紀大腸菌研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 試験管内転写tRNAによる遺伝暗号改変タンパク質合成系の構築2017

    • Author(s)
      日比 敬太、網藏 和晃、清水 義宏、横川 隆志、上田 卓也
    • Organizer
      第14回21世紀大腸菌研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞骨格因子群の人工膜小胞内合成2016

    • Author(s)
      古里 匠、堀江 史博、松林 英明、車 ゆうてつ、網藏 和晃、上田 卓也
    • Organizer
      「細胞を創る」研究会 9.0
    • Place of Presentation
      早稲田大学(東京、新宿区)
    • Year and Date
      2016-11-21
    • Related Report
      2016 Research-status Report
  • [Presentation] 試験管内転写されたtRNAによるタンパク質合成系の構築2016

    • Author(s)
      日比敬太, 網蔵和晃, 清水義宏, 上田卓也
    • Organizer
      第11回無細胞生命科学研究会
    • Place of Presentation
      ゆこたんの森(岩手、雫石町)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
  • [Presentation] 試験管内再構成実験によるバクテリオリボソームの生合成過程の解明2016

    • Author(s)
      網藏和晃、青山遼、田丸大知、清水義宏、上田卓也
    • Organizer
      第4回 Ribosome Meeting
    • Place of Presentation
      大阪医科大学(大阪、高槻市)
    • Year and Date
      2016-09-17
    • Related Report
      2016 Research-status Report
  • [Presentation] バクテリアリボソームの試験管内再構成2016

    • Author(s)
      網藏和晃
    • Organizer
      日本進化学会弟18回大会
    • Place of Presentation
      東京工業大学(東京、目黒区)
    • Year and Date
      2016-08-25
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Single-molecule studies of ribosome assembly: Coupling transcription and assembly2016

    • Author(s)
      Ueda, T., Aoyama, R., Amikura, K., Bercy, M., Bizebard, T., Bockelmann, U., Biebricher, A., Wuite, G.J.L., Peterman, E.J.G., Nikolay, R., Hilal, T., Bo, Q., and Nierhaus, K.H.
    • Organizer
      the 16th HFSP Awardees Meeting
    • Place of Presentation
      Biopolice, Singapore
    • Year and Date
      2016-07-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Single-molecule studies of ribosome assembly: Coupling transcription and assembly2016

    • Author(s)
      Ueda, T., Aoyama, R., Amikura, K., Bercy, M., Bizebard, T., Bockelmann, U., Biebricher, A., Wuite, G.J.L., Peterman, E.J.G., Nikolay, R., Hilal, T., Bo, Q., and Nierhaus, K.H.
    • Organizer
      the 16th HFSP Awardees Meeting
    • Place of Presentation
      Singapore
    • Year and Date
      2016-07-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] In vitro reconstitution of 30S ribosomal subunit in the presence of ribosome biogenesis factors2016

    • Author(s)
      Kazuaki Amikura, Aoyama Ryo, Tamaru Daichi, Shimizu Yoshihiro, Ueda Takuya
    • Organizer
      EMBO conference ribosome structure and function 2016
    • Place of Presentation
      Strasbourg, France
    • Year and Date
      2016-07-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Reconstitution of the early step assembly of E.coli 50S ribosomal subunit in vitro2016

    • Author(s)
      Aoyama Ryo, Kazuaki Amikura, Ueda Takuya
    • Organizer
      EMBO conference ribosome structure and function 2016
    • Place of Presentation
      Stransbourg, France
    • Year and Date
      2016-07-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] In vitro reconstitution of 30S ribosomal subunit in the presence of ribosome biogenesis factors2016

    • Author(s)
      Amikura Kazuaki, Aoyama Ryo, Tamaru Daichi, Shimizu Yoshihiro, Ueda Takuya
    • Organizer
      EMBO conference:Ribosome structure and function 2016
    • Place of Presentation
      France
    • Year and Date
      2016-07-06
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Reconstitution of the early step assembly of E.coli 50S ribosomal subunit in vitro2016

    • Author(s)
      Ryo aoyama, Kazuaki Amikura, Takuya Ueda
    • Organizer
      EMBO conference:Ribosome structure and function 2016
    • Place of Presentation
      France
    • Year and Date
      2016-07-06
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] リボソームの30Sサブユニットのin vitro再構成手法2016

    • Author(s)
      網藏和晃、青山遼、田丸大知、清水義宏、上田卓也
    • Organizer
      第13回 21世紀大腸菌研究会
    • Place of Presentation
      グリーンピア(熊本、南阿蘇)
    • Year and Date
      2016-06-02
    • Related Report
      2016 Research-status Report
  • [Presentation] 50S サブユニットのin vitro再構成に向けた実験ツールの構築2016

    • Author(s)
      青山遼, 網藏和晃, 上田卓也
    • Organizer
      第13回 21世紀大腸菌研究会
    • Place of Presentation
      グリーンピア(熊本、南阿蘇)
    • Year and Date
      2016-06-02
    • Related Report
      2016 Research-status Report
  • [Presentation] 全ての生命が有する翻訳系の起源2016

    • Author(s)
      網蔵和晃
    • Organizer
      第4回 宇宙における生命ワークショップ
    • Place of Presentation
      東京
    • Year and Date
      2016-03-07
    • Related Report
      2015 Research-status Report
  • [Presentation] 大腸菌50Sサブユニットのアセンブリー初期過程の再現2015

    • Author(s)
      青山遼、網藏和晃、上田卓也
    • Organizer
      第10回無細胞生命科学研究会
    • Place of Presentation
      神奈川
    • Year and Date
      2015-10-13
    • Related Report
      2015 Research-status Report
  • [Presentation] 単純化遺伝暗号:限られたアミノ酸セットで構成されるタンパク質配列空間を探索するための進化分子工学的ツール2015

    • Author(s)
      網藏和晃
    • Organizer
      日本進化学会 第17回大会
    • Place of Presentation
      東京
    • Year and Date
      2015-08-20
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] 無細胞タンパク質合成系による生命システムの再構成2017

    • Author(s)
      網蔵和晃, 上田卓也
    • Total Pages
      215
    • Publisher
      シーエムシー出版
    • Related Report
      2016 Research-status Report
  • [Book] 理大 科学フォーラム2015

    • Author(s)
      網藏和晃 木賀大介
    • Total Pages
      4
    • Publisher
      東京理科大学
    • Related Report
      2015 Research-status Report
  • [Remarks] Kazuaki Amikura

    • URL

      https://sites.google.com/site/amikura1986/

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2021-01-27  

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