The establishment and its application of the evaluation system of disease-associated SNP using a reverse genetics technique

Research Project

Project/Area Number	15K16123
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Risk sciences of radiation and chemicals
Research Institution	Hiroshima University
Principal Investigator	Yanagihara Hiromi 広島大学, 原爆放射線医科学研究所, 助教 (50719474)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	ゲノム編集 / SNP / NBS1
Outline of Final Research Achievements	We planed to establish the new experimental system to analyze disease-associated single nucleotide polymorphisms (SNPs) using a reverse genetics technique. In this study, we developed a method to generate SNP model cells using CRISPR/Cas9 system. This method has enabled us to carry out comparative study of candidate SNP in human cultured cells under genetically uniform backgrounds. The SNP model cell lines we established in this study, which have either mono- or bi-allelic mutation, showed increased chromosome instability.

Report

(3 results)