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Analysis of radiation induced mutation patterns and frequencies with whole genome sequencing

Research Project

Project/Area Number 15K16125
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation and chemicals
Research InstitutionHiroshima University

Principal Investigator

Kanai Akinori  広島大学, 原爆放射線医科学研究所, 助教 (60549567)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords放射線 / 全ゲノム解析
Outline of Final Research Achievements

To estimate radiation induced mutation patterns and frequencies with whole genome sequencing, HT1080 single cells were gamma-irradiated 84 mGy, 390 mGy and 1885 mGy. The cells were cultured, DNA was extracted and we performed whole genome sequencing using HiSeq2500. Large deletions were rarely detected and there was no difference with dose dependent. The numbers of snps, small insertions and deletions were increased in 1885 mGy irradiated cell.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015

All Journal Article (9 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 9 results,  Open Access: 8 results,  Acknowledgement Compliant: 4 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] A novel LSD1 inhibitor NCD38 ameliorates MDS-related leukemia with complex karyotype by attenuating leukemia programs via activating super-enhancers.2017

    • Author(s)
      Sugino N, Kawahara M, Tatsumi G, Kanai A, Matsui H, Yamamoto R, Nagai Y, Fujii S, Shimazu Y, Hishizawa M, Inaba T, Andoh A, Suzuki T, Takaori-Kondo A.
    • Journal Title

      Leukemia

      Volume: in press Issue: 11 Pages: 2303-2314

    • DOI

      10.1038/leu.2017.59

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acquired expression of CblQ367P in mice induces dysplastic myelopoiesis mimicking chronic myelomonocytic leukemia2017

    • Author(s)
      Nakata Y, Ueda T, Nagamachi A, Yamasaki N, Ikeda KI, Sera Y, Takubo K, Kanai A, Oda H, Sanada M, Ogawa S, Tsuji K, Ebihara Y, Wolff L, Honda ZI, Suda T, Inaba T, Honda H.
    • Journal Title

      Blood

      Volume: 129 Issue: 15 Pages: 2148-2160

    • DOI

      10.1182/blood-2016-06-724658

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Propagation of tri-methylated H3K27 regulated by EED is required for embryogenesis, hematopoietic maintenance, and tumor suppression.2016

    • Author(s)
      Ueda T, Nakata Y, Nagamachi A, Yamasaki N, Kanai A, Matsui H, Honda ZI, Oda H, Wolff L, Inaba T, Honda H.
    • Journal Title

      PNAS

      Volume: 113(37) Issue: 37 Pages: 10370-10375

    • DOI

      10.1073/pnas.1600070113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Maintenance of the functional integrity of mouse hematopoiesis by EED and promotion of leukemogenesis by EED haploinsufficiency2016

    • Author(s)
      Ikeda K-I, Ueda T, Yamasaki N, Nakata Y, Sera Y, Nagamachi A, Miyama T, Kobayashi H, TakuboK, Kanai A, Oda H, Wolff l, Honda Z-1, Ichinohe T, Matsubara A, Suda T, Inaba T, Honda H
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 29454-29454

    • DOI

      10.1038/srep29454

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The loss of Ezh2 cooperates with an active JAK2 mutant in the pathogenesis of myelofibrosis and sensitizes tumor-initiating cells to bromodomain inhibition.2016

    • Author(s)
      Sashida G, Wang S, Tomioka T, Oshima M, Kazumasa Aoyama K, Kanai A, Mochizuki-Kashio M, Harada H, Shimoda K, Iwama A.
    • Journal Title

      J Experimenta l Medicine

      Volume: 213 Issue: 8 Pages: 1459-1477

    • DOI

      10.1084/jem.20151121

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] ALKR1275Q perturbs extracellular matrix, enhances cell invasion and leads to the development of neuroblastoma in cooperation with MYCN.2016

    • Author(s)
      Ueda T, Nakata Y, Yamasaki N, Oda H, Sentani K, Kanai A, Onishi N, Ikeda K, Sera Y, Honda ZI, Tanaka K, Sata M, Ogawa S, Yasui W, Saya H, Takita J, Honda H.
    • Journal Title

      Oncogene.

      Volume: 無 Issue: 34 Pages: 4447-4458

    • DOI

      10.1038/onc.2015.519

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] H-NS Facilitates Sequence Diversification of Horizontally Transferred DNAs during Their Integration in Host Chromosomes2016

    • Author(s)
      Higashi K., Tobe T., Kanai A., Uyar E., Ishikawa S., Suzuki Y., Ogasawara N., Kurokawa K., and Oshima T
    • Journal Title

      PLoS genetics

      Volume: 12 Issue: 1 Pages: e1005796-e1005796

    • DOI

      10.1371/journal.pgen.1005796

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] AF4 uses the SL1 components of RNAP1 machinery to initiate MLL fusion- and AEP-dependent transcription.2015

    • Author(s)
      Okuda H, Kanai A, Ito S, Matsui H, Yokoyama A.
    • Journal Title

      Nat Commun.

      Volume: 23;6 Issue: 1

    • DOI

      10.1038/ncomms9869

    • NAID

      120005672585

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Fbxl10 overexpression in murine hematopoietic stemcells induces leukemia involving metabolic activation and upregulation of Nsg22015

    • Author(s)
      Ueda T, Nagamachi A, Takubo K, Yamasaki N, Matsui H, Kanai A, Nakata Y, Ikeda K Konuma T, Oda H, Wolff L, Honda Z, Wu X, Helin K, Iwama A, Suda T, Inaba T, Honda H.
    • Journal Title

      Blood

      Volume: 125 Issue: 22 Pages: 3437-3446

    • DOI

      10.1182/blood-2014-03-562694

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Analysis of radiation induced mutations using next generation sequencer.2017

    • Author(s)
      Akinori Kanai, Akiko Nagamachi, Hidehiko Kawai, Hirotaka Matsui, Kenji Kamiya, Toshiya Inaba
    • Organizer
      放射線災害・医科学研究拠点 第 1 回国際シンポジウム
    • Place of Presentation
      広島市 広島大学
    • Year and Date
      2017-02-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いた放射線誘発変異解析2016

    • Author(s)
      金井昭教、長町安希子、河合秀彦、松井啓隆、神谷研二、稲葉俊哉
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      横浜市 パシフィコ横浜 広島市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いた放射線誘発変異解析2016

    • Author(s)
      金井昭教、長町安希子、河合秀彦、松井啓隆、神谷研二、稲葉俊哉
    • Organizer
      日本放射線影響学会第59回大会
    • Place of Presentation
      JMSアステールプラザ
    • Year and Date
      2016-10-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いた放射線誘発変異解析2015

    • Author(s)
      金井昭教、長町安希子、河合秀彦、松井啓隆、神谷研二、稲葉俊哉
    • Organizer
      第40回中国地区放射線影響研究会
    • Place of Presentation
      広島大学
    • Year and Date
      2015-07-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 次世代シーケンサーを用いた放射線誘発変異解析2015

    • Author(s)
      金井昭教、長町安希子、河合秀彦、松井啓隆、神谷研二、稲葉俊哉
    • Organizer
      NGS現場の会第四回研究会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-07-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 次世代シーケンサーを用いた放射線誘発変異解析2015

    • Author(s)
      金井昭教、長町安希子、河合秀彦、松井啓隆、神谷研二、稲葉俊哉
    • Organizer
      平成27年度広島大学・長崎大学連携研究カンファランス
    • Place of Presentation
      広島大学
    • Year and Date
      2015-06-06
    • Related Report
      2015 Research-status Report
  • [Presentation] Analysis of radiation induced mutations using next generation sequencer.2015

    • Author(s)
      Akinori Kanai, Akiko Nagamachi, Hidehiko Kawai, Hirotaka Matsui, Kamiya Kenji, Toshiya Inaba
    • Organizer
      15th International Congress of Radiation Research (ICRR)
    • Place of Presentation
      Kyoto International Conference Center
    • Year and Date
      2015-05-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2018-03-22  

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