Structural analysis of histone proteins extracted from DNA damaged cells using circular dichroism spectroscopy

• Project/Area Number: 15K16130
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Hiroshima University (2016); Japan Atomic Energy Agency (2015)
• Principal Investigator: Izumi Yudai 広島大学, 放射光科学研究センター, 助教 (20595772)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: DNA損傷応答 / ヒストン / 円二色性 / 翻訳後修飾 / 放射光 / 放射線生物学

Outline of Final Research Achievements

In an attempt to investigate structural alterations of histone proteins induced by DNA damage responses, we measured circular dichroism spectra of histones extracted from DNA-damaged cells. We revealed that DNA damage response increases α-helix structure in histone H2A-H2B, but decreases that in histone H3-H4. Since different structural alterations were observed in H2A-H2B and H3-H4, it is suggested that the structural alterations of histones are caused by the several pathways. Interestingly, these altered structures persisted for at least 24 hours. These structural alterations would play an important role in DNA damage responses, such as DNA damage repair pathways.

Research Products

• [Int'l Joint Research] Synchrotron SOLEIL/University of Paris South/University of Pierre and Marie Curie(フランス)
• [Int'l Joint Research] Synchrotron SOLEIL/University of Paris South/University of Pierre and Marie Curie(フランス)
• [Journal Article] DNA damage response induces structural alterations of histone H3-H4 (2017)
  • Author(s): Y. Izumi, K. Fujii, S. Yamamoto, K. Matsuo, H. Namatame, M. Taniguchi, and A. Yokoya
  • Journal Title: Journal of Radiation Research Volume: 58 Issue: 1 Pages: 59-65
  • DOI: 10.1093/jrr/rrw086
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural transition from β-strand and turn to α-helix in histone H2A-H2B induced by DNA damage response (2016)
  • Author(s): Y. Izumi, K. Fujii, F. Wien, C. Houee-Levin, S. Lacombe, D. Salado-Leza, E. Porcel, R. Masoud, S. Yamamoto, M. Refegiers, M. -A. Herve du Penhoat, and A. Yokoya
  • Journal Title: Biophysics Journal Volume: 111 Issue: 1 Pages: 69-78
  • DOI: 10.1016/j.bpj.2016.06.002
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 放射線DNA損傷の修復応答を開始させる染色体タンパク質ヒストンの構造変化 (2016)
  • Author(s): 泉 雄大、山本悟史、藤井健太郎、横谷明徳
  • Journal Title: Isotope News Volume: 746 Pages: 2-5
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 紫外円二色性スペクトル測定を用いたタンパク質構造研究 (2016)
  • Author(s): 泉 雄大、山本悟史、藤井健太郎、横谷明徳
  • Journal Title: 放射線生物研究 Volume: 51 Pages: 91-106
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Secondary structure alterations of histones H2A and H2B in X-irradiated cancer cells: Altered histones persist in cells for at least 24 hours (2015)
  • Author(s): Y. izumi, S. Yamamoto, K. Fujii, and A. Yokoya
  • Journal Title: Radiation Research Volume: 184 Issue: 5 Pages: 554-558
  • DOI: 10.1667/rr14071.1
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Structural changes of histoneinduced by DNA damage response (2017)
  • Author(s): Y. Izumi
  • Organizer: Hiroshima International Workshop on Circular Dichroism Spectroscopy 2017 ~Application to Imaging Microscopy and Radiation Biology~
  • Place of Presentation: 広島大学（広島県東広島市）
  • Year and Date: 2017-02-28
  • Int'l Joint Research
• [Presentation] 放射光円二色性分光によるDNA損傷誘起ヒストン二次構造変化の観測 (2017)
  • Author(s): 泉 雄大、山本悟史、藤井健太郎、松尾光一、横谷明徳
  • Organizer: 第30回日本放射光学会年会 放射光科学合同シンポジウム
  • Place of Presentation: 神戸芸術センター（兵庫県神戸市）
  • Year and Date: 2017-01-07
• [Presentation] DNA損傷応答により生じるヒストンの二次構造変化 (2016)
  • Author(s): 泉 雄大、山本悟史、藤井健太郎、松尾光一、横谷明徳
  • Organizer: 日本放射線影響学会第59回大会
  • Place of Presentation: JMSアステールプラザ（広島県広島市）
  • Year and Date: 2016-10-26
• [Presentation] DNA損傷により誘起されるヒストンタンパク質の二次構造変化 (2016)
  • Author(s): 泉 雄大、山本悟史、藤井健太郎、松尾光一、横谷明徳
  • Organizer: 第59回放射線化学討論会
  • Place of Presentation: QST高崎量子応用研究所（群馬県高崎市）
  • Year and Date: 2016-09-20
• [Presentation] Secondary structural alteration of histone H2A-H2B induced by DNA damage responses (2016)
  • Author(s): Y. Izumi, S. Yamamoto, K. Fujii, and A. Yokoya
  • Organizer: 14th International Workshop on Radiation Damage to DNA
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2016-03-20
  • Int'l Joint Research
• [Presentation] VUV-CD measurements of histone H2A-H2B proteins: Secondary structural alteration induced by DNA damage responses (2016)
  • Author(s): Y. Izumi
  • Organizer: The 20th Hiroshima International Symposium on Synchrotron Radiation
  • Place of Presentation: 広島大学（広島県東広島市）
  • Year and Date: 2016-03-10
  • Int'l Joint Research / Invited
• [Remarks] 研究成果のプレスリリースページ
  • URL: http://www.jaea.go.jp/02/press2015/p15110501/