Establishment of ozone risk assessment model for leaf CO2 assimilation of woody plant

• Project/Area Number: 15K16136
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental impact assessment
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Watanabe Makoto 東京農工大学, (連合)農学研究科(研究院), 特任准教授 (50612256)
• Research Collaborator: IZUTA Takeshi 東京農工大学, 大学院農学研究院, 教授 (20212946) ; KOIKE Takayoshi 北海道大学, 大学院農学研究院, 教授 (10270919)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: オゾン / 樹木 / 光合成 / リスク評価 / CO2固定

Outline of Final Research Achievements

Ozone is an air pollutant that negatively affects tree growth. The responses of photosynthetic function to elevated ozone were studied to establish a model for estimating ozone effects on CO2 sequestration of trees. Ozone-induced reduction of photosynthetic activity was due not to stomatal closure, but mainly due to biochemical limitation. Furthermore, in some tree species, the ozone sensitivity of photosynthesis in upper canopy leaves was higher than that in lower canopy leaves.

Research Products

• [Journal Article] Effects of simulated nitrogen deposition on ectomycorrhizae community structure in hybrid larch and its parents grown in volcanic ash soil: The role of phosphorous (2018)
  • Author(s): Wang Xiaona、Agathokleous Evgenios、Qu Laiye、Fujita Saki、Watanabe Makoto、Tamai Yutaka、Mao Qiaozhi、Koyama Akihiro、Koike Takayoshi
  • Journal Title: Science of the Total Environment Volume: 618 Pages: 905-915
  • DOI: 10.1016/j.scitotenv.2017.08.283
  • NAID: 120006812529
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Growth and photosynthetic response of two larches exposed to O3 mixing ratios ranging from preindustrial to near future (2018)
  • Author(s): Sugai T.、Kam D.-G.、Agathokleous E.、Watanabe M.、Kita K.、Koike T.
  • Journal Title: Photosynthetica Volume: 印刷中 Issue: 3 Pages: 901-910
  • DOI: 10.1007/s11099-017-0747-7
  • Peer Reviewed
• [Journal Article] Photosynthesis of three evergreen broad-leaved tree species, Castanopsis sieboldii, Quercus glauca,and Q. myrsinaefolia, under elevated ozone (2018)
  • Author(s): Watanabe M.、Kinose Y.、Izuta T.
  • Journal Title: iForest Volume: 印刷中
  • Peer Reviewed / Open Access
• [Journal Article] オゾンおよび高濃度CO2に対する樹木の生理生態学的応答 (2018)
  • Author(s): 渡辺 誠
  • Journal Title: 大気環境学会誌 Volume: 53 Pages: 62-69
  • NAID: 130006734394
• [Journal Article] Ozone alters the feeding behavior of the leaf beetle Agelastica coerulea (Coleoptera: Chrysomelidae) into leaves of Japanese white birch (Betula platyphylla var. japonica) (2017)
  • Author(s): Eugenios Agathokleous, Tetsuichi Sakikawa, Shahenda A. Abu ElEla, Tomoki Mochizuki, Masahiro Nakamura, Makoto Watanabe, Kimitaka Kawamura, Takayoshi Koike
  • Journal Title: Environmental Science and Pollution Research Volume: 24(21) Issue: 21 Pages: 17577-17583
  • DOI: 10.1007/s11356-017-9369-7
  • NAID: 120006484247
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ozone-induced stomatal sluggishness changes stomatal parameters of Jarvis-type model in white birch and deciduous oak (2017)
  • Author(s): Hoshika Y.、Watanabe M.、Carrari E.、Paoletti E.、Koike T.
  • Journal Title: Plant Bioloby Volume: 20 Issue: 1 Pages: 20-28
  • DOI: 10.1111/plb.12632
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Monoterpene emissions from needles of hybrid larch F1 (Larix gmelinii var. japonica × Larix kaempferi) grown under elevated carbon dioxide and ozone. (2017)
  • Author(s): Mochizuki T, Watanabe M, Koike T, and Tani A
  • Journal Title: Atmospheric Environment Volume: 148 Pages: 197-202
  • DOI: 10.1016/j.atmosenv.2016.10.041
  • Peer Reviewed
• [Journal Article] Nutrient supply to soil offsets the ozone-induced growth reduction in Fagus crenata seedlings (2017)
  • Author(s): Kinose, Y., Fukamachi, Y., Okabe, S., Hiroshima, H., Watanabe, M. and Izuta, T.
  • Journal Title: Trees (Structure and Function) Volume: 31 Issue: 1 Pages: 259-272
  • DOI: 10.1007/s00468-016-1481-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Photosynthetic responses to ozone of upper and lower canopy leaves of Fagus crenata Blume seedlings grown under different soil nutrient conditions (2017)
  • Author(s): Kinose, Y., Fukamachi, Y., Okabe, S., Hiroshima, H., Watanabe, M. and Izuta, T.
  • Journal Title: Environmental Pollution Volume: 223 Pages: 213-222
  • DOI: 10.1016/j.envpol.2017.01.014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effects of CO₂ and O₃ on the interaction between root of woody plants and ectomycorrhizae (2016)
  • Author(s): Wang, X., Agathokleous, E., Qu, L., Watanabe, M. and Koike, T.
  • Journal Title: Journal of Agricultural Meteorology Volume: 72 Issue: 2 Pages: 95-105
  • DOI: 10.2480/agrmet.D-14-00045
  • NAID: 130005156598
  • ISSN: 0021-8588, 1881-0136
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Leaf phenology and insect grazing of Japanese white birch saplings grown under free-air ozone exposure (2016)
  • Author(s): Sakikawa T, Shi C, Nakamura M, Watanabe M, Oikawa M, Satoh F, Koike T
  • Journal Title: Journal of Agricultural Meteorology Volume: 72 Issue: 2 Pages: 80-84
  • DOI: 10.2480/agrmet.D-14-00031
  • NAID: 130005156600
  • ISSN: 0021-8588, 1881-0136
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A meta-analysis of leaf nitrogen distribution within plant canopies (2016)
  • Author(s): Hikosaka, K., Anten, NPR., Borjigidai, A., Kamiyama, C., Sakai, H., Hasegawa, T., Oikawa, S., Iio, A., Watanabe, M., Koike, T., Nishina, K., Ito, A.
  • Journal Title: Annals of Botany Volume: 118 Issue: 2 Pages: 239-247
  • DOI: 10.1093/aob/mcw099
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 大気環境と植物－第4講樹木に対するオゾンの影響－ (2016)
  • Author(s): 渡辺 誠, 山口真弘, 伊豆田 猛
  • Journal Title: 大気環境学会誌 Volume: 51
  • NAID: 130005834735
  • Acknowledgement Compliant
• [Journal Article] Foliar chemical composition of two oak species grown in a free-air enrichment system with elevated O₃ and CO₂ (2016)
  • Author(s): Shi, C., Kitao, M., Agathokleous, E., Watanabe, M., Tobita, H., Yazaki, K., Kitaoka, S. and Koike, T.
  • Journal Title: Journal of Agricultural Meteorology Volume: 72 Issue: 1 Pages: 50-58
  • DOI: 10.2480/agrmet.D-14-00018
  • NAID: 130005132481
  • ISSN: 0021-8588, 1881-0136
  • Peer Reviewed / Open Access
• [Journal Article] A review study on past 40 years of research on effects of tropospheric O3 on belowground structure, functioning and processes of trees: a linkage with potential ecological implications (2016)
  • Author(s): Agathokleous, E., Saitanis, CJ., Wang, X., Watanabe, M. and Koike, T.
  • Journal Title: Water, Air, & Soil Pollution Volume: 227 Issue: 1 Pages: 1-28
  • DOI: 10.1007/s11270-015-2715-9
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ozone-induced stomatal sluggishness changes carbon and water balance of temperate deciduous forests (2015)
  • Author(s): Hoshika, Y., Katata, G., Deushi, M., Watanabe, M., Koike, T. and Paoletti, E.
  • Journal Title: Scientific Reports Volume: 5(9871) Issue: 1 Pages: 1-8
  • DOI: 10.1038/srep09871
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ethylene-di-urea (EDU), an effective phytoproctectant against O₃ deleterious effects and a valuable research tool (2015)
  • Author(s): Agathokleous, E., Koike, T., Watanabe, M., Hoshika, Y. and Saitanis, CJ.
  • Journal Title: Journal of Agricultural Meteorology Volume: 71 Issue: 3 Pages: 185-195
  • DOI: 10.2480/agrmet.D-14-00017
  • NAID: 130005098163
  • ISSN: 0021-8588, 1881-0136
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of ozone-induced stomatal closure on ozone uptake and its changes due to leaf age in sun and shade leaves of Siebold's beech (2015)
  • Author(s): Hoshika, Y., Watanabe, M., Inada, N. and Koike, T.
  • Journal Title: Journal of Agricultural Meteorology Volume: 71 Issue: 3 Pages: 218-226
  • DOI: 10.2480/agrmet.D-14-00013
  • NAID: 130005098164
  • ISSN: 0021-8588, 1881-0136
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Difference in photosynthetic responses to free air ozone fumigation between upper and lower canopy leaves of Japanese oak (<i>Quercus mongolica</i> var. <i>crispula</i>) saplings (2015)
  • Author(s): Watanabe, M., Hoshika, Y., Inada, N. and Koike, T.
  • Journal Title: Journal of Agricultural Meteorology Volume: 71 Issue: 3 Pages: 227-231
  • DOI: 10.2480/agrmet.D-14-00012
  • NAID: 130005098169
  • ISSN: 0021-8588, 1881-0136
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Growth of Japanese and hybrid larch seedlings grown under free-air O₃ fumigation—an initial assessment of the effects of adequate and excessive nitrogen (2015)
  • Author(s): Kam, DG., Shi, C., Watanabe, M., Kita, K., Satoh, F. and Koike, T.
  • Journal Title: Journal of Agricultural Meteorology Volume: 71 Issue: 3 Pages: 239-244
  • DOI: 10.2480/agrmet.D-14-00029
  • NAID: 130005098171
  • ISSN: 0021-8588, 1881-0136
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ethylenediurea (EDU) as a protectant of plants against O3 (2015)
  • Author(s): Agathokleous, E., Koike, T., Saitanis, CJ., Watanabe, M., Satoh, F. and Hoshika, Y.
  • Journal Title: Eurasian Journal of Forest Research. Volume: 18 Pages: 37-50
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Forest declining of mountain birch in norther Japan: Examination of effect of the elevated ozone on birch (2018)
  • Author(s): Koike T.、Sakuma A.、Tatsuta S.、Hoshika Y.、 Watanabe M.
  • Organizer: 日本生態学会第65回全国大会
• [Presentation] ブナの葉内光合成過程に対するオゾンの影響 (2018)
  • Author(s): 渡辺 誠、上牧 祐、岡部成晃、森 麻里乃、伊豆田 猛
  • Organizer: 第120回日本森林学会大会
• [Presentation] Difference in ozone susceptibility of photosynthesis between upper and lower canopy leaves of Siebold's beech (2017)
  • Author(s): Watanabe M.、Kinose Y.、Hoshika Y.、Koike T.、Izuta T.
  • Organizer: XIX International Botanical Congress
  • Int'l Joint Research / Invited
• [Presentation] Ozone-induced stomatal sluggishness changes carbon and water balance of temperate deciduous forests (2017)
  • Author(s): Hoshika Y.、Katata G.、Watanabe M.、Deushi M.、Koike T.、Paoletti E.
  • Organizer: IUFRO 125th Anniversary Congress 2017
  • Int'l Joint Research
• [Presentation] Nitrogen use efficiency for growth of Fagus crenata seedlings under elevated ozone with different soil nutrient conditions (2017)
  • Author(s): Watanabe M.、Hiroshima H.、Kinose Y.、Okabe S.、Izuta T
  • Organizer: IUFRO Tokyo 2017: Actions for sustainable forest ecosystems under air pollution and climate change
  • Int'l Joint Research
• [Presentation] Farquhar-Ball モデルによるブナ苗の葉のオゾン吸収量の推定 (2017)
  • Author(s): 黄瀬佳之、深町吉伸、渡辺 誠、伊豆田 猛
  • Organizer: 第58回大気環境学会年会
• [Presentation] 日本の森林樹種の成長および乾物分配のオゾンに対する応答 (2017)
  • Author(s): 渡辺 誠, 黄瀬佳之, 星加康智, Evgenios Agathokleous, 小池孝良, 松村秀幸, 河野吉久, 伊豆田 猛
  • Organizer: 第128回日本森林学会大会
  • Place of Presentation: 鹿児島大学(鹿児島市)
• [Presentation] ブナ苗の成長における窒素利用効率に対するオゾンの影響 (2016)
  • Author(s): 渡辺 誠, 廣島ひろか, 黄瀬佳之, 岡部成晃, 伊豆田 猛
  • Organizer: 第127回日本森林学会大会
  • Place of Presentation: 日本大学（藤沢市）
  • Year and Date: 2016-03-28
• [Presentation] ブナ苗の上位葉と下位葉の光合成能力に対するオゾンの影響 (2016)
  • Author(s): 黄瀬佳之, 岡部成晃, 廣島ひろか, 渡辺 誠, 伊豆田 猛
  • Organizer: 第127回日本森林学会大会
  • Place of Presentation: 日本大学（藤沢市）
  • Year and Date: 2016-03-28
• [Presentation] ブナ苗の根の成長および植栽土壌の呼吸速度に対するオゾンと土壌養分の単独及び複合影響 (2016)
  • Author(s): 岡部成晃, 黄瀬佳之, 廣島ひろか, 伊豆田 猛, 渡辺 誠
  • Organizer: 第127回日本森林学会大会
  • Place of Presentation: 日本大学（藤沢市）
  • Year and Date: 2016-03-28
• [Presentation] 養分状態が異なる土壌で育成したブナ苗の上位葉と下位葉の光合成能力に対するオゾンの影響 (2016)
  • Author(s): 黄瀬佳之, 岡部成晃, 廣島ひろか, 渡辺 誠, 伊豆田 猛
  • Organizer: 第57回大気環境学会年会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] 養分状態が異なる土壌で育成したブナ苗の成長に対するオゾンの影響 (2016)
  • Author(s): 黄瀬佳之, 岡部成晃, 廣島ひろか, 渡辺 誠, 伊豆田 猛
  • Organizer: 第57回大気環境学会年会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] 異なる土壌養分条件下で育成したブナ苗の植栽土壌における呼吸速度に対するオゾンの影響 (2016)
  • Author(s): 岡部成晃, 黄瀬佳之, 廣島 ひろか, 伊豆田 猛, 渡辺 誠
  • Organizer: 第57回大気環境学会年会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] 異なる土壌養分条件におけるブナ苗の成長における窒素利用効率に対するオゾンの影響 (2016)
  • Author(s): 渡辺 誠, 廣島ひろか, 黄瀬佳之, 岡部成晃, 伊豆田 猛
  • Organizer: 第57回大気環境学会年会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] コマツナの成長に対するサブミクロンサイズの硫酸アンモニウム粒子の影響 (2016)
  • Author(s): 母袋 朗, 渡辺 誠, 伊豆田 猛
  • Organizer: 第57回大気環境学会年会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] 高濃度CO2、O3、それら複合曝露がグイマツ雑種F1のモノテルペン放出に及ぼす影響 (2016)
  • Author(s): 望月智貴, 渡辺 誠, 小池孝良, 谷 晃
  • Organizer: 第57回大気環境学会年会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] Global ozone impacts on forest carbon and water use (2016)
  • Author(s): Hoshika, Y., Katata, G., Watanabe, M., Deushi, M., Koike, T. and Paoletti, E.
  • Organizer: IUFRO Regional Congress for Asia and Oceania 2016, Forest for Sustainable Development: The Role of Research
  • Place of Presentation: China National Convention Centre (中国北京市)
  • Int'l Joint Research
• [Presentation] Nitrogen use efficiency for growth of Fagus crenata seedlings under elevated ozone with different soil nutrient conditions (2016)
  • Author(s): Watanabe, M., Hiroshima, H., Kinose, Y., Okabe, S. and Izuta, T.
  • Organizer: The 2nd Asian Air Pollution Workshop
  • Place of Presentation: Research Center for Eco-Environmental Sciences Chinese Academy of Sciences (中国北京市)
  • Int'l Joint Research
• [Presentation] Nutrient supply to soil offsets the ozone-induced reduction in growth of Fagus crenata seedlings (2016)
  • Author(s): Kinose, Y., Okabe, S., Hiroshima, H., Watanabe, M. and Izuta, T.
  • Organizer: The 2nd Asian Air Pollution Workshop
  • Place of Presentation: Research Center for Eco-Environmental Sciences Chinese Academy of Sciences (中国北京市)
  • Int'l Joint Research
• [Presentation] 植物葉群内における葉間窒素分配のメタ解析 (2015)
  • Author(s): 彦坂幸毅, Anten, NPR., Borjigidai, A., 神山千穂, 酒井英光, 長谷川利拡, 及川真平, 飯尾淳弘, 渡辺 誠, 小池孝良, 仁科一哉, 伊藤昭彦
  • Organizer: 東北植物学会第5回大会
  • Place of Presentation: 福島大学（福島市）
  • Year and Date: 2015-12-19
• [Presentation] Difference in photosynthetic responses of Siebold's beech (Fagus crenata) and Japanese oak (Quercus mongolica var. crispula) saplings to free air ozone fumigation (2015)
  • Author(s): Watanabe, M., Hoshika, Y. and Koike, T.
  • Organizer: The 1st Asian Air Pollution Workshop
  • Place of Presentation: 東京大学（文京区）
  • Year and Date: 2015-10-03
  • Int'l Joint Research
• [Presentation] Toward modeling of stomatal conductance under elevated ozone in forest trees (2015)
  • Author(s): Hoshika, Y., Watanabe, M., Katata, G., De Marco, A., Deushi, M., Carriero, G., Koike, T. and Paoletti, E.
  • Organizer: 10th congress of Italian Society of Silviculture and Forest Ecology
  • Place of Presentation: Universita degli Studi di Firenze（イタリア、フィレンツェ）
  • Year and Date: 2015-09-16
  • Int'l Joint Research
• [Presentation] 着葉位置の異なるミズナラ葉の光合成に対するオゾンの影響 (2015)
  • Author(s): 渡辺 誠, 星加康智, 稲田直輝, 小池孝良
  • Organizer: 第56回大気環境学会年会
  • Place of Presentation: 早稲田大学（新宿区）
  • Year and Date: 2015-09-15
• [Presentation] ブナの光合成パラメータに対するオゾンの影響評価 (2015)
  • Author(s): 黄瀬佳之, 深町吉伸, 渡辺 誠, 伊豆田 猛
  • Organizer: 第56回大気環境学会年会
  • Place of Presentation: 早稲田大学（新宿区）
  • Year and Date: 2015-09-15
• [Presentation] スダジイ、アラカシおよびシラカシ苗のオゾンに対する成長・光合成応答 (2015)
  • Author(s): 渡辺 誠, 黄瀬佳之, 伊豆田 猛
  • Organizer: 第56回大気環境学会年会
  • Place of Presentation: 早稲田大学（新宿区）
  • Year and Date: 2015-09-15
• [Presentation] To develop stomatal conductance modeling under elevated ozone in forest trees (2015)
  • Author(s): Hoshika, Y., Watanabe, M., Carriero, G., Koike, T. and Paoletti, E.
  • Organizer: IUFRO 2015: Grobal Challenges of Air Pollution and Climate Change to Forests
  • Place of Presentation: Hyatt Regency Nice Palais de la Mediterranean（フランス、ニース）
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] Plant defense and photosynthesis of Japanese white birch saplings grown under a free-air O3 fumigation system (2015)
  • Author(s): Sakikawa, T., Nakamura, M., Watanabe, M.., Kanie, S., Satoh, F. and Koike, T.
  • Organizer: IUFRO 2015: Grobal Challenges of Air Pollution and Climate Change to Forests
  • Place of Presentation: Hyatt Regency Nice Palais de la Mediterranean（フランス、ニース）
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] Difference in photosynthetic responses to ozone between canopy positions in Japanese oak (2015)
  • Author(s): Watanabe, M., Hoshika, Y., Inada, N. and Koike, T.
  • Organizer: IUFRO 2015: Grobal Challenges of Air Pollution and Climate Change to Forests
  • Place of Presentation: Hyatt Regency Nice Palais de la Mediterranean（フランス、ニース）
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] Relationship between cumulative stomatal ozone uptake and photosynthetic parameters of Fagus crenata (2015)
  • Author(s): Kinose, Y., Fukamachi, Y., Watanabe, M. and Izuta, T.
  • Organizer: IUFRO 2015: Grobal Challenges of Air Pollution and Climate Change to Forests
  • Place of Presentation: Hyatt Regency Nice Palais de la Mediterranean（フランス、ニース）
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] Growth of Japanese larch and its hybrid saplings grown under different O3 levels (2015)
  • Author(s): Kam, DG., Watanabe, M., Yamaguchi, M., Watanabe, T., Satoh, F. and Koike, T.
  • Organizer: IUFRO 2015: Grobal Challenges of Air Pollution and Climate Change to Forests
  • Place of Presentation: Hyatt Regency Nice Palais de la Mediterranean（フランス、ニース）
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] Response of Japanese forest tree species to elevated ozone (2015)
  • Author(s): Watanabe, M., Hoshika, Y. and Koike, T.
  • Organizer: The second international forum on sustainable development of subtropical forest: forest ecology and environment protection
  • Place of Presentation: Zhejiang A&F University （中国臨安市）
  • Year and Date: 2015-04-09
  • Int'l Joint Research / Invited