| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
To establish a bitter sensor for high-throughput evaluation, the easier method with common instruments such as microplate reader was applied in my previous study. In this study, I have improved this method.
First, I found out that dysfunction of a transfected gene was responsible for the low responsibility. The response to bitter tastant salicin was increased to 2.3 times by establishing stably expressed cell lines, and was increased to 1.6 times by modifying transfection condition of the gene. At the end of further modification of transfecting all genes, the response was increased to 22% of that to positive control reagent.
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