The physiological significance of the change in biomembrane fatty acids according to nutritional state

• Project/Area Number: 15K16203
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Ochanomizu University
• Principal Investigator: Ikuyo Ichi お茶の水女子大学, 基幹研究院, 講師 (50403316)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脂肪酸 / 必須脂肪酸 / 脂肪滴 / リン脂質 / 多価不飽和脂肪酸

Outline of Final Research Achievements

In this study, we examined that the biological significance of biomembrane fatty acids via the excess or deficiency of fatty acids. First, we focused the phospholipid fatty acids in lipid droplets of adipocytes as a model of over nutrition. We clarified that the biophysical properties of lipids themselves constituting lipid droplets contributed to the formation of large lipid droplets. Furthermore, Our results suggest that saturated fatty acid chains in phospholipid monolayers might establish the form and/or stability of large LDs. In addition, as a model of nutritional deficiency, we examined the change of fatty acid metabolism during essential fatty acid deficiency. We revealed that Elovl5 of fatty acid elongation enzyme changed the substrate specificity via phosphorylation modification and induced the synthesis of Mead acid (C20:3n-9) in essential fatty acid deficiency.

Research Products

• [Journal Article] Ezetimibe in Combination With Statins Ameliorates Endothelial Dysfunction in Coronary Arteries After Stenting: The CuVIC Trial (Effect of Cholesterol Absorption Inhibitor Usage on Target Vessel Dysfunction After Coronary Stenting), a Multicenter Randomized Controlled Trial (2017)
  • Author(s): S. Takase, T. Matoba, S. Nakashiro, Y. Mukai, S. Inoue, K. Oi, T. Higo, S. Katsuki, M. Takemoto, N. Suematsu, K. Eshima, K. Miyata, M. Yamamoto, M. Usui, K. Sadamatsu, S. Satoh, T. Kadokami, K .Hironaga, I. Ichi, K. Todaka, J. Kishimoto, K. Egashira, K. Sunagawa
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 37 Issue: 2 Pages: 350-358
  • DOI: 10.1161/atvbaha.116.308388
  • Peer Reviewed
• [Journal Article] Oleuropein improves insulin resistance in skeletal muscle by promoting the translocation of GLUT4 (2017)
  • Author(s): Y. Fujiwara, C. Tsukahara, N. Ikeda, Y. Sone, T. Ishikawa, I. Ichi, T. Koike, and Y. Aoki
  • Journal Title: J. Clin. Biochem. Nutr. Volume: 印刷中
  • NAID: 130006191915
  • Peer Reviewed
• [Journal Article] Fatty acid composition profiles of 235 strains of 3 plantae phylum within the NIES algal collection (2017)
  • Author(s): E. Mitani, F. Nakayama, I. Matsuwaki, I. Ichi, A. Kawabata, M. Kawachi, M. Kato
  • Journal Title: Microb. Resour. Syst. Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Saturated fatty acid in the phospholipid monolayer contributes to the formation of large lipid droplets (2016)
  • Author(s): Kotoko Arisawa, Haruka Mitsudome, Konomi Yoshida, Shizuka Sugimoto, Tomoko Ishikawa, Yoko Fujiwara, Ikuyo Ichi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 480 Issue: 4 Pages: 641-647
  • DOI: 10.1016/j.bbrc.2016.10.109
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] TTC39B Deficiency Stabilizes LXR Leading to Decreased Atherosclerosis and Hepatic Steatosis (2016)
  • Author(s): M. Koseki, J. Hsieh, M. M. Molusky, E. Yakushiji, I. Ichi, M. Westerterp, S. Abramowicz, L. Tascau, C. B. Welch, J. Iqbal, S. Takiguchi, S. Yamashita, M. M. Hussain, D. J. Rader, and A. R. Tall
  • Journal Title: Nature Volume: 535 Issue: 7611 Pages: 303-307
  • DOI: 10.1038/nature18628
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] TTC39B Deficiency Stabilizes LXR Leading to Decreased Atherosclerosis and Hepatic Steatosis (2016)
  • Author(s): Masahiro Koseki, Joanne Hsieh1, Matthew M. Molusky, Emi Yakushiji, Ikuyo Ichi, Marit Westerterp, Sandra Abramowicz, Liana Tascau, Carrie B. Welch, Jahangir Iqbal, Shunichi Takiguchi, Shizuya Yamashita, M. Mahmood Hussain, Daniel J. Rader, and Alan R. Tall1
  • Journal Title: Nature Volume: in press
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 脂肪滴膜リン脂質の脂肪酸不飽和度が脂肪滴膜タンパク質の局在移行に与える影響 (2017)
  • Author(s): 有澤琴子、市 育代、藤原葉子
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-17
• [Presentation] 必須脂肪酸欠乏におけるElovl5のリン酸化を介した活性制御機構の解明 (2017)
  • Author(s): 林優里、市 育代、山野美怜、藤原葉子
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-17
• [Presentation] 多価不飽和脂肪酸によるElovl5の活性制御機構の解明 (2016)
  • Author(s): 林優里、市 育代、山野美怜、藤原葉子
  • Organizer: 第39回日本分子生物学会年会11月30日～12月2日
  • Place of Presentation: 横浜
  • Year and Date: 2016-11-30
• [Presentation] 脂肪滴の肥大化と膜リン脂質および中性脂肪の脂肪酸との関連性 (2016)
  • Author(s): 市 育代、有澤琴子、満留 悠、藤原葉子
  • Organizer: 日本ビタミン学会第68回大会
  • Place of Presentation: 富山
  • Year and Date: 2016-06-17
• [Presentation] 必須脂肪酸欠乏における脂肪酸伸長酵素Elovl5の活性制御機構 (2016)
  • Author(s): 林 優里、市 育代、山野美怜、藤原葉子
  • Organizer: 第70回日本栄養・食糧学会
  • Place of Presentation: 兵庫
  • Year and Date: 2016-05-13
• [Presentation] 脂肪滴における膜リン脂質の脂肪酸組成とPerilipin1の局在との関連性 (2016)
  • Author(s): 有澤琴子、市 育代、満留 悠、藤原葉子
  • Organizer: 第70回日本栄養・食糧学会
  • Place of Presentation: 兵庫
  • Year and Date: 2016-05-13
• [Presentation] LPS誘発性炎症モデルにおけるdesmosterolの蓄積と炎症に対する作用 (2016)
  • Author(s): 小林恭子、市 育代、永瀬摩奈、藤原葉子
  • Organizer: 第70回日本栄養・食糧学会
  • Place of Presentation: 兵庫
  • Year and Date: 2016-05-13
• [Presentation] 必須脂肪酸欠乏の脂肪酸代謝と調節機構 (2016)
  • Author(s): 市 育代
  • Organizer: 第19回日本病態栄養学会
  • Place of Presentation: 神奈川県・横浜市
  • Year and Date: 2016-01-09
  • Invited
• [Presentation] 脂肪滴における中性脂肪およびリン脂質の脂肪酸組成と脂肪滴サイズの関連性 (2015)
  • Author(s): 有澤琴子, 市 育代, 満留悠，藤原葉子
  • Organizer: BMB2015
  • Place of Presentation: 兵庫県・神戸市
  • Year and Date: 2015-12-01
• [Presentation] 酸化ステロールの骨格筋細胞における糖代謝への影響 (2015)
  • Author(s): 柳本彩，市 育代，藤原葉子
  • Organizer: BMB2015
  • Place of Presentation: 兵庫県・神戸市
  • Year and Date: 2015-12-01
• [Presentation] 必須脂肪酸欠乏における皮膚炎症とミード酸産生遺伝子との関連性 (2015)
  • Author(s): 多田萌, 市 育代，藤原葉子
  • Organizer: BMB2015
  • Place of Presentation: 兵庫県・神戸市
  • Year and Date: 2015-12-01
• [Presentation] ステント留置後の冠動脈疾患患者におけるエゼチミブの血清酸化コレステロールの減少作用 (2015)
  • Author(s): 市 育代，藤原葉子
  • Organizer: 第37回日本臨床栄養学会総会
  • Place of Presentation: 東京都・千代田区
  • Year and Date: 2015-10-02
• [Presentation] 脂肪酸鎖長伸長酵素Elovl5の多価不飽和脂肪酸による伸長活性の変化 (2015)
  • Author(s): 市 育代、山野美怜、藤原葉子
  • Organizer: 日本ビタミン学会代67回大会
  • Place of Presentation: 奈良県・奈良市
  • Year and Date: 2015-06-17
• [Presentation] 食品に含まれるステロールと動脈硬化について (2015)
  • Author(s): 市 育代
  • Organizer: 第15回日本抗加齢医学会総会
  • Place of Presentation: 福岡県・福岡市
  • Year and Date: 2015-05-29
  • Invited
• [Presentation] Ezetimibe, a cholesterol absorption inhibitor, reduces serum oxysterol in patients with coronary artery undergoing stent placement (2015)
  • Author(s): Aya Yanagimoto, Ikuyo Ichi, Sakie Igarashi, Tetsuya Matoba, Susumu Takase, Kensuke Egashira and Yoko Fujiwara
  • Organizer: ACN2015 12th Asian Congress of Nutrition
  • Place of Presentation: 神奈川県・横浜市
  • Year and Date: 2015-05-14
  • Int'l Joint Research
• [Book] 栄養科学シリーズNEXT 臨床栄養学実習第2版 (2017)
  • Author(s): 塚原丘美（分担執筆）
  • Total Pages: 165
  • Publisher: 講談社
• [Book] 新スタンダード栄養・食物シリーズ9 基礎栄養学 (2015)
  • Author(s): 池田彩子、鈴木恵美子、背山洋右、野口忠、藤原葉子
  • Total Pages: 10
  • Publisher: 東京化学同人