The role of astrocyte lipid rafts in mental disorder induced by a lack of nutrition

• Project/Area Number: 15K16206
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Tohoku University
• Principal Investigator: Kagawa Yoshiteru 東北大学, 医学系研究科, 助教 (30728887)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脂肪酸結合蛋白質 / FABP7 / アストロサイト / 脂質ラフト / caveolin-1 / 神経可塑性 / ヒストンアセチル化 / エピゲノム

Outline of Final Research Achievements

Recently, the relationship between higher brain functions and neurological disorder has been paid attention. It is reported that a lack of nutrition such as n-3 polyunsaturated fatty acids (PUFAs) induces mental disorders, but its mechanism is still unknown. In this study, we focused the fatty acid binding protein (FABP7) which is highly expressed in astrocytes and has high affinity with n-3 PUFAs. We examined whether FABP7 is involved in the functions of lipid rafts which is the main source of cellular activity in response to external stimuli. The following results have been so far obtained: 1. FABP7 controls lipid raft function through regulation of caveolin-1 expression and is involved in the response of astrocytes to the external stimuli, 2. altered production of humoral factors from FABP7-KO astrocytes induced neuroplasticity changes.

Research Products

• [Journal Article] Ultrastructural histometric evidence for expansion of the sustentacular cell envelope in response to hypersecretion of adrenal chromaffin cells in mice. (2016)
  • Author(s): Pakkarato S, Thoungseabyoun W, Tachow A, Rawangwong A, Kagawa Y, Owada Y, Kondo H, Hipkaeo W.
  • Journal Title: Anat Sci Int. Volume: 印刷中
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 脳型脂肪酸結合蛋白質によるアストロサイトの膜微小ドメイン機能制御 (2016)
  • Author(s): 香川慶輝, 尾形雅君, Majid Ebrahimi, 大和田祐二
  • Journal Title: Proceedings Clinical Electron Microscopy Volume: 34 Pages: 1-4
  • Acknowledgement Compliant
• [Journal Article] Astrocyte-expressed FABP7 regulates dendritic morphology and excitatory synaptic function of cortical neurons. (2016)
  • Author(s): Ebrahimi M, Yamamoto Y, Sharifi K, Kida H, Kagawa Y, Yasumoto Y, Islam A, Miyazaki H, Shimamoto C, Maekawa M, Mitsushima D, Yoshikawa T, Owada Y.
  • Journal Title: Glia Volume: 64(1) Issue: 1 Pages: 48-62
  • DOI: 10.1002/glia.22902
  • Peer Reviewed / Open Access
• [Journal Article] Dorsal forebrain-specific deficiency of Reelin-Dab1 signal causes behavioral abnormalities related to psychiatric disorders. (2016)
  • Author(s): Imai H, Shoji H, Ogata M, Kagawa Y, Owada Y, Miyakawa T, Sakimura K, Terashima T, Katsuyama Y.
  • Journal Title: Cereb Cortex Volume: in press
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Inhibition of fatty acid synthase decreases expression of stemness markers in glioma stem cells. (2016)
  • Author(s): Yasumoto Y., Miyazaki H., Vaidyan L.K., Kagawa Y., Ebrahimi M., Yamamoto Y., Ogata M., Katsuyama Y., Sadahiro H., Suzuki M., & Owada Y.
  • Journal Title: PLoS One Volume: 11(1) Issue: 1 Pages: 11-11
  • DOI: 10.1371/journal.pone.0147717
  • Peer Reviewed / Open Access
• [Journal Article] Omega-3 fatty acid transport through the placenta. (2016)
  • Author(s): Islam A, Kodama T, Yamamoto Y, Ebrahimi M, Miyazaki H, Yasumoto Y, Kagawa Y, Sawada T, Owada Y, Tokuda N.
  • Journal Title: Asian J Med Biol Res Volume: in press
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Proteomic differential display analysis reveals decreased expression of PEA-15 and vimentin in FABP7-deficient astrocytes. (2015)
  • Author(s): Ebrahimi, M., Sharifi, K., Islam, A., Miyazaki, H., Yasumoto, Y., Kagawa, Y., Yamamoto, Y., Kitagawa, T., Kuramitsu, Y., Nakamura, K. and Owada, Y.
  • Journal Title: J Proteom Bioinform. Volume: 8 Issue: 01 Pages: 9-14
  • DOI: 10.4172/jpb.1000347
  • Peer Reviewed / Open Access
• [Journal Article] Immunohistochemical analysis of sustentacular cells in the adrenal medulla, carotid body and sympathetic ganglion of mice using an antibody against brain-type fatty acid binding protein (B-FABP). (2015)
  • Author(s): Pakkarato S, Chomphoo S, Kagawa Y, Owada Y, Mothong W, Iamsaard S, Sawatpanich T, Kondo H, Hipkaeo W.
  • Journal Title: J Anat Volume: 226 Issue: 4 Pages: 348-353
  • DOI: 10.1111/joa.12285
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fatty acid-binding protein 7 regulates function of caveolae in astrocytes through expression of caveolin-1. (2015)
  • Author(s): Yoshiteru Kagawa, Yuki Yasumoto, Kazem Sharifi, Majid Ebrahimi, Ariful Islam, Hirofumi Miyazaki, Yui Yamamoto, Tomoo Sawada, Hiroko Kishi, Sei Kobayashi, Motoko Maekawa, Takeo Yoshikawa, Eiichi Takaki, Akira Nakai, Hiroshi Kogo, Toyoshi Fujimoto and Yuji Owada
  • Journal Title: Glia Volume: 63 Issue: 5 Pages: 780-794
  • DOI: 10.1002/glia.22784
  • Peer Reviewed / Open Access
• [Journal Article] A novel trigger for cholesterol-dependent smooth muscle contraction mediated by the sphingosylphosphorylcholine-Rho-kinase pathway in the rat basilar artery: a mechanistic role for lipid rafts (2015)
  • Author(s): Satoshi Shirao, Hiroshi Yoneda, Mizuya Shinoyama, Kazutaka Sugimoto, Hiroyasu Koizumi, Hideyuki Ishihara, Fumiaki Oka, Hirokazu Sadahiro, Sadahiro Nomura, Masami Fujii, Masakatsu Tamechika, Yoshiteru Kagawa, Yuji Owada, and Michiyasu Suzuki
  • Journal Title: Journal of Cerebral Blood Flow and Metabolism Volume: 印刷中 Issue: 5 Pages: 1-8
  • DOI: 10.1038/jcbfm.2014.260
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 細胞核内FABP7はcaveolin-1遺伝子発現をエピジェネティックに制御する (2017)
  • Author(s): 香川慶輝, Ariful Islam, 尾形雅君, 大和田祐二
  • Organizer: 第122回 日本解剖学会総会
  • Place of Presentation: 長崎大学（長崎県、長崎市）
  • Year and Date: 2017-03-28
• [Presentation] 腸上皮細胞間リンパ球(IEL)の抗体刺激に伴う絨毛上皮細胞の剥離機構 (2017)
  • Author(s): 尾形雅君, 香川慶輝, 安本有希, 伊藤恒敏, 大和田祐二
  • Organizer: 第122回 日本解剖学会総会
  • Place of Presentation: 長崎大学（長崎県、長崎市）
  • Year and Date: 2017-03-28
• [Presentation] Significance of FABP7 and Ndufs4 in the regulation of astrocyte mitochondorial dynamics and fucntions. (2017)
  • Author(s): Angelina Misiou, Fumika Nanto, Yoshiteru Kagawa, Takaaki Abe, Yuji Owada
  • Organizer: Euro-Japan double degree program International Workshop
  • Place of Presentation: 京都府立医科大学（京都府、京都市）
  • Year and Date: 2017-01-25
  • Int'l Joint Research
• [Presentation] 核内FABP7はエピジェネティックなcaveolin-1の発現制御に関与する (2016)
  • Author(s): 香川慶輝, Ariful Islam, 尾形雅君, 大和田祐二
  • Organizer: 第39回 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県、横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 脂肪酸結合蛋白質FABP7の細胞核局在の意義ーcaveolin-1遺伝子発現制御との関連ー (2016)
  • Author(s): 香川慶輝, Ariful Islam, 尾形雅君, 大和田祐二
  • Organizer: 第62回 日本解剖学会 東北・北海道連合支部学術集会
  • Place of Presentation: 帯広畜産大学（北海道、帯広市）
  • Year and Date: 2016-09-03
• [Presentation] 腸上皮間リンパ球(IEL)における自己作用型DNA傷害およびDNA修復 (2016)
  • Author(s): 尾形雅君, 香川慶輝, 安本有希, 伊藤恒敏, 大和田祐二
  • Organizer: 第62回 日本解剖学会 東北・北海道連合支部学術集会
  • Place of Presentation: 帯広畜産大学（北海道、帯広市）
  • Year and Date: 2016-09-03
• [Presentation] 脂肪酸結合蛋白質FABP7の細胞内局在がcaveolin-1遺伝子発現に及ぼす影響 (2016)
  • Author(s): 香川慶輝、大和田祐二
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビックパレットふくしま（福島市）
  • Year and Date: 2016-03-28
• [Presentation] Ultimate fate of mouse small intestinal intra-epithelial lymphocytes (2016)
  • Author(s): 尾形雅君、勝山裕、香川慶輝、伊藤恒敏、大和田祐二
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビックパレットふくしま（福島市）
  • Year and Date: 2016-03-28
• [Presentation] 大脳皮質特異的Dab1欠損マウスは精神疾患関連行動異常を示す (2016)
  • Author(s): 勝山裕、今井英明、昌子浩孝、尾形雅君、香川慶輝、崎村健司、宮川剛、大和田祐二、寺島俊雄
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビックパレットふくしま（福島市）
  • Year and Date: 2016-03-28
• [Presentation] FABP7 is an epigenetic modulator of caveolin-1 gene expression in astrocytes. (2015)
  • Author(s): Yoshiteru Kagawa, Yuji Owada
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場、神戸国際展示場（神戸市）
  • Year and Date: 2015-07-28
• [Remarks] 東北大学大学院医学系研究科器官解剖学分野ホームページ
  • URL: http://www.organ-anatomy.med.tohoku.ac.jp/index.html