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Investigation of mechanism of dietary ligands-TRPA1 interactions

Research Project

Project/Area Number 15K16212
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Eating habits
Research InstitutionOkayama University

Principal Investigator

NAKAMURA Toshiyuki  岡山大学, 環境生命科学研究科, 助教 (90706988)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsフラボノイド / イソチオシアネート / TRPA1
Outline of Final Research Achievements

We investigated the interaction between TRPA1 and food compounds. We indicated some novel agonists of TRPA1 such as Kaempferol and Myricetin. In addition, the agonistic activities of several major quercetin glycosides and metabolites towards TRPA1 were also evaluated. Methylated quercetin activated TRPA1 receptor. To identify the site of interaction of these flavonoids and TRPA1, we attempted to prepare the monoclonal antibodies to flavonoids. We developed a novel monoclonal antibody using dihydroxyphenylacetic acid as an immunogen.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2016 2015

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite.2016

    • Author(s)
      Nakashima, S., Liu, Z., Yamaguchi, Y., Saiki, S., Munemasa, S., Nakamura, T., Murata, Y., Nakamura, Y.
    • Journal Title

      Biochem. Biophys. Rep.

      Volume: 7 Pages: 240-245

    • DOI

      10.1016/j.bbrep.2016.06.020

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Activation of transient receptor potential ankyrin 1 by quercetin and its analogs.2016

    • Author(s)
      Toshiyuki Nakamura, Noriyuki Miyoshi, Takeshi Ishii, Miyu Nishikawa, Shinichi Ikushiro, Tatsuo Watanabe
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 80 Issue: 5 Pages: 949-954

    • DOI

      10.1080/09168451.2015.1132148

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 食品成分の相互作用と代謝と局在と機能性2016

    • Author(s)
      中村俊之
    • Organizer
      Food Science Forum
    • Place of Presentation
      岡山
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Quercetin and its analogues activates TRPA1.2015

    • Author(s)
      Toshiyuki Nakamura, Noriyuki Miyoshi, Takeshi Ishii, Miyu Nishikawa, Shinichi Ikushiro, Tatsuo Watanabe
    • Organizer
      International Conference on Food Factors
    • Place of Presentation
      Soul, Korea
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Modulation of TRPA1 activity by flavonoids2015

    • Author(s)
      Toshiyuki Nakamura, Noriyuki Miyoshi, Takeshi Ishii, Shinichi Ikushiro, Tatsuo Watanabe
    • Organizer
      International Symposium on Dietary Antioxidants and Oxidative Stress in Health 2015
    • Place of Presentation
      Awaji, Japan
    • Year and Date
      2015-08-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2018-03-22  

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