Investigation of mechanism of dietary ligands-TRPA1 interactions

• Project/Area Number: 15K16212
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Okayama University
• Principal Investigator: NAKAMURA Toshiyuki 岡山大学, 環境生命科学研究科, 助教 (90706988)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: フラボノイド / イソチオシアネート / TRPA1

Outline of Final Research Achievements

We investigated the interaction between TRPA1 and food compounds. We indicated some novel agonists of TRPA1 such as Kaempferol and Myricetin. In addition, the agonistic activities of several major quercetin glycosides and metabolites towards TRPA1 were also evaluated. Methylated quercetin activated TRPA1 receptor. To identify the site of interaction of these flavonoids and TRPA1, we attempted to prepare the monoclonal antibodies to flavonoids. We developed a novel monoclonal antibody using dihydroxyphenylacetic acid as an immunogen.

Research Products

• [Journal Article] A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite. (2016)
  • Author(s): Nakashima, S., Liu, Z., Yamaguchi, Y., Saiki, S., Munemasa, S., Nakamura, T., Murata, Y., Nakamura, Y.
  • Journal Title: Biochem. Biophys. Rep. Volume: 7 Pages: 240-245
  • DOI: 10.1016/j.bbrep.2016.06.020
  • Peer Reviewed / Open Access
• [Journal Article] Activation of transient receptor potential ankyrin 1 by quercetin and its analogs. (2016)
  • Author(s): Toshiyuki Nakamura, Noriyuki Miyoshi, Takeshi Ishii, Miyu Nishikawa, Shinichi Ikushiro, Tatsuo Watanabe
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 80 Issue: 5 Pages: 949-954
  • DOI: 10.1080/09168451.2015.1132148
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 食品成分の相互作用と代謝と局在と機能性 (2016)
  • Author(s): 中村俊之
  • Organizer: Food Science Forum
  • Place of Presentation: 岡山
  • Year and Date: 2016-09-08
  • Invited
• [Presentation] Quercetin and its analogues activates TRPA1. (2015)
  • Author(s): Toshiyuki Nakamura, Noriyuki Miyoshi, Takeshi Ishii, Miyu Nishikawa, Shinichi Ikushiro, Tatsuo Watanabe
  • Organizer: International Conference on Food Factors
  • Place of Presentation: Soul, Korea
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] Modulation of TRPA1 activity by flavonoids (2015)
  • Author(s): Toshiyuki Nakamura, Noriyuki Miyoshi, Takeshi Ishii, Shinichi Ikushiro, Tatsuo Watanabe
  • Organizer: International Symposium on Dietary Antioxidants and Oxidative Stress in Health 2015
  • Place of Presentation: Awaji, Japan
  • Year and Date: 2015-08-30
  • Int'l Joint Research