Investigation of mechanism of dietary ligands-TRPA1 interactions
Project/Area Number |
15K16212
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Eating habits
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Research Institution | Okayama University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | フラボノイド / イソチオシアネート / TRPA1 |
Outline of Final Research Achievements |
We investigated the interaction between TRPA1 and food compounds. We indicated some novel agonists of TRPA1 such as Kaempferol and Myricetin. In addition, the agonistic activities of several major quercetin glycosides and metabolites towards TRPA1 were also evaluated. Methylated quercetin activated TRPA1 receptor. To identify the site of interaction of these flavonoids and TRPA1, we attempted to prepare the monoclonal antibodies to flavonoids. We developed a novel monoclonal antibody using dihydroxyphenylacetic acid as an immunogen.
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Report
(3 results)
Research Products
(5 results)
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[Journal Article] A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite.2016
Author(s)
Nakashima, S., Liu, Z., Yamaguchi, Y., Saiki, S., Munemasa, S., Nakamura, T., Murata, Y., Nakamura, Y.
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Journal Title
Biochem. Biophys. Rep.
Volume: 7
Pages: 240-245
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Quercetin and its analogues activates TRPA1.2015
Author(s)
Toshiyuki Nakamura, Noriyuki Miyoshi, Takeshi Ishii, Miyu Nishikawa, Shinichi Ikushiro, Tatsuo Watanabe
Organizer
International Conference on Food Factors
Place of Presentation
Soul, Korea
Year and Date
2015-11-22
Related Report
Int'l Joint Research
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[Presentation] Modulation of TRPA1 activity by flavonoids2015
Author(s)
Toshiyuki Nakamura, Noriyuki Miyoshi, Takeshi Ishii, Shinichi Ikushiro, Tatsuo Watanabe
Organizer
International Symposium on Dietary Antioxidants and Oxidative Stress in Health 2015
Place of Presentation
Awaji, Japan
Year and Date
2015-08-30
Related Report
Int'l Joint Research