Fabrication of a microfluidic device for circulating tumor cell sensing
| Project/Area Number |
15K16320
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
TABATA Miyuki 東京医科歯科大学, 生体材料工学研究所, テニュアトラック助教 (00636839)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | バイオセンサ / 血中循環腫瘍細胞 / 乳がん |
|---|
| Outline of Final Research Achievements |
In this research, we performed to fabricate a compact and portable device that detect circulating tumor cells (CTC) and analyze nucleic acids using ultra-sensitive biosensor based-on the principle of field effect transistors (FETs). Model breast cancer epithelial cells were immobilized on the sensor which the probe molecules were immobilized, and then electrical measurement was performed. Because cancer cells have a negative charge derived from sialic acids, we succeeded in electrically discriminating cancer cells and epithelial cells according to the difference in the amount of sialic acid expression on cell membrane. Furthermore, we tried to develop an early diagnosis device for cancer by analyzing the nucleic acid.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Real-time Monitoring and Detection of Primer Generation-Rolling Circle Amplification of DNA Using an Ethidium Ion-selective Electrode2016
Author(s)
Ayaka Seichi, Nanami Kozuka, Yuko Kashima, Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Naoko Iwasawa, Daniel Citterio, Yuji Miyahara, Koji Suzuki
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Journal Title
Analytical Sciences
Volume: 32
Issue: 5
Pages: 505-510
DOI
NAID
ISSN
0910-6340, 1348-2246
Related Report
Peer Reviewed
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[Presentation] Real-time monitoring and detection of primer generation-rolling circle amplification of DNA using an ethidium ion selective electrode2015
Author(s)
Ayaka Seichi, Nanami Kozuka, Yuko Kashima, Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Naoko Iwasawa, Daniel Citterio, Yuji Miyahara, Koji Suzuki
Organizer
The 16th Beijing Conference and Exhibision on Instrumental Analysis (BCEIA2015)
Place of Presentation
Beijing, China
Year and Date
2015-10-27
Related Report
Int'l Joint Research
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