Biological nano imaging in wetcondition using cathodoluminescence of rare-earth doped nanophosphors

• Project/Area Number: 15K16323
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Osaka University
• Principal Investigator: Furukawa Taichi 大阪大学, ナノサイエンスデザイン教育研究センター, 特任助教(常勤) (70749043)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: カソードルミネッセンス / バイオイメージング / 希土類 / ナノ粒子 / 蛍光体 / 均一沈殿法

Outline of Final Research Achievements

We developed specimen chamber, rare-earth doped nanophosphors, and new CL detection system for CL bioimaging in liquid condition. Biological CL imaging in liquid condition was achieved by developing specimen chamber having SiN thin membrane. Mono-dispersed nanophosphors with size of about 40 nm were obtained by homogeneous precipitation method using excess urea. In addition, we developed new mirror for efficient CL detection, and the mirror was mounted in SEM.

Research Products

• [Journal Article] カソードルミネッセンス顕微鏡と近赤外顕微鏡によるマルチモーダルイメージング (2017)
  • Author(s): 新岡宏彦，福島昌一郎，古川太一，橋本守
  • Journal Title: 光アライアンス Volume: 28 Pages: 24-28
  • NAID: 40021036876
• [Journal Article] Correlative near-infrared light and cathodoluminescence microscopy using Y2O3:Ln, Yb (Ln = Tm, Er) nanophosphors for multi-scale, multi-colour bioimaging (2016)
  • Author(s): Fukushima, S., Furukawa, T., Niioka, H., Ichimiya, M., Sannomiya, T., Tanaka, N., Onoshima, D., Yukawa, H., Baba, Y., Miyake, J., Ashida, M., Araki, T., and Hashimoto, M.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 25950-25950
  • DOI: 10.1038/srep25950
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Multispectral Emissions of Lanthanide-Doped Gadolinium Oxide Nanophosphors for Cathodoluminescence and Near-Infrared Upconversion/Downconversion Imaging (2016)
  • Author(s): D. T. K. Dung, S. Fukushima, T. Furukawa, H. Niioka*, T. Sannomiya, K. Kobayashi, H. Yukawa, Y. Baba, M. Hashimoto, and J. Miyake
  • Journal Title: Nanomaterials Volume: 6 Issue: 9 Pages: 163-163
  • DOI: 10.3390/nano6090163
  • Peer Reviewed / Open Access
• [Journal Article] Synthesis of Y2O3 nanophosphors by homogeneous precipitation method using excessive urea for cathodoluminescence and upconversion luminescence bioimaging (2016)
  • Author(s): S. Fukushima, T. Furukawa, H. Niioka, M. Ichimiya, T. Sannomiya, J. Miyake, M. Ashida, T. Araki, and M. Hashimoto
  • Journal Title: Optical Materials Express Volume: 6 Issue: 3 Pages: 831-843
  • DOI: 10.1364/ome.6.000831
  • Peer Reviewed / Open Access
• [Presentation] Imaging of 3D cellular spheroids by trimodalities CL-NIR-MRI (2016)
  • Author(s): D. T. K. Dung, S. Fukushima, T. Furukawa, H. Niioka, Y. Mori, Y. Yoshioka, M. Hashimoto, and J. Miyake
  • Organizer: NFO-14
  • Place of Presentation: アクトシティ浜松(静岡県・浜松市)
  • Year and Date: 2016-09-04
• [Presentation] Multi-scaled bioimaging with near-infrared luminescence and cathodoluminescence (2016)
  • Author(s): S. Fukushima, T. Furukawa, H. Nioka, M. Ichimiya, T. Sannomiya, M. Ashida, J. Miyake, T. Araki, and M. Hashimoto
  • Organizer: 生体医用画像研究会第3回若手発表会
  • Place of Presentation: 大阪大学基礎工学部シグマホール (豊中市，大阪府)
  • Year and Date: 2016-03-02
• [Presentation] カソードルミネッセンス顕微鏡を用いた液中ナノバイオイメージング (2016)
  • Author(s): 古川 太一，福島 昌一郎，新岡 宏彦，一宮 正義，芦田 昌明，三宅 淳，橋本 守
  • Organizer: 日本機械学会 第28回バイオエンジニアリング講演会
  • Place of Presentation: 東京工業大学 (大岡山，東京)
  • Year and Date: 2016-01-09
• [Presentation] 希土類添加蛍光体の電子線励起発光を用いた液中生体ナノイメージング (2015)
  • Author(s): 古川 太一，福島 昌一郎，新岡 宏彦，一宮 正義，芦田 昌明，三宅 淳，橋本 守
  • Organizer: 医用分光学研究会
  • Place of Presentation: ホテルグランドヒル市ヶ谷 (市ヶ谷，東京)
  • Year and Date: 2015-12-03
• [Presentation] Rare-earth doped Y2O3 nano-phosphor probes for correlative cathodoluminescence and near-infrared optical bio-imaging (2015)
  • Author(s): H. Niioka, S. Fukushima, T. Furukawa, M. Ichimiya, M. Ashida, J. Miyake, and M. Hashimoto
  • Organizer: The 2nd East-Asia Microscopy Conference
  • Place of Presentation: The Himeji Chamber of Commerce and Industry (Himeji, Hyogo, JAPAN)
  • Year and Date: 2015-11-24
  • Int'l Joint Research
• [Presentation] Biological high-resolution imaging in wetcondition using cathodoluminescence microscopy (2015)
  • Author(s): T. Furukawa, S. Fukushima, H. Niioka, M. Ichimiya, J. Miyake, M. Ashida , T.Araki, M. Hashimoto
  • Organizer: Optics & Photonics International Congress 2015
  • Place of Presentation: Pacifico-Yokohama (Yokohama, Kanagawa, JAPAN)
  • Year and Date: 2015-04-21
  • Int'l Joint Research