| Project/Area Number |
15K16336
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical systems
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| Research Institution | Tohoku University |
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Principal Investigator |
Ito-Sasaki Takahiro 東北大学, 原子分子材料科学高等研究機構, 研究支援者 (90400449)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 等温増幅 / 簡易遺伝子検査 / 核酸クロマトグラフ / 多検体検出 / プローブを作製 / 環状化酵素の選択 / 増幅酵素の選択 / 断片化酵素の選択 / 増幅シグナルの検出に成功 |
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| Outline of Final Research Achievements |
In this study, I developed simplified gene detection system to use clinical site. Then I researched "RCA-PAS method" that used isothermal amplification and one of the paper chromatogragh, STH-PAS. Although RCA method that used in this study had two steps, circularization and amplification, I succeeded to combine these reaction to optimize reactive condition. This combination was contributed to cut the time needed for reaction. Then I tested STH-PAS detection with amplification solution. It was detected the objective signal on STH-PAS and it was succeeded to construct the simplified gene detection system.
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