Response of skeletal muscle in mice and hamster to ketogenic diet feeding
| Project/Area Number |
15K16499
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Sports science
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| Research Institution | The University of Tokushima (2017)
National Institute of Advanced Industrial Science and Technology (2015-2016) |
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Principal Investigator |
NAKAO Reiko 徳島大学, 大学院医歯薬学研究部(医学系), 講師 (20582696)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ケトン体食 / 熱産生 / 筋萎縮 / 体内時計 |
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| Outline of Final Research Achievements |
We found that the mRNA expression of Slc25a25, a Ca2+-sensitive ATP carrier in the inner mitochondrial membrane, fluctuates in a circadian manner in mouse skeletal muscle and it was damped in Clock mutant and Bmal1-deficient mice. Furthermore, a ketogenic diet (KD) that induces time-of-day-dependent hypothermia, induced Slc25a25 mRNA expression in skeletal muscle. Sciatic denervation abolished circadian and KD-induced Slc25a25 expression. We measured body temperature (Tb) in sciatic denervated mice fed with KD to determine the functional role of KD-induced Slc25a25 expression. Sciatic denervation abolished Slc25a25 expression and augmented KD-induced hypothermia compared with sham-operated mice. These findings suggest that KD feeding induces expression of the muscle circadian gene Slc25a25 via neural pathways, and that SLC25A25 might be involved in muscle thermogenesis under KD-induced hypothermia in mice.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Short-term feeding at the wrong time is sufficient to desynchronize peripheral clocks and induce obesity with hyperphagia, physical inactivity and metabolic disorders in mice2016
Author(s)
Yasumoto Y, Hashimoto C, Nakao R, Yamazaki H, Hiroyama H, Nemoto T, Yamamoto S, Sakurai M, Oike H, Wada N, Yoshida-Noro C, Oishi K
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Journal Title
Metabolism
Volume: 65
Issue: 5
Pages: 714-727
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Structural analysis of the TKB domain of ubiquitin ligase Cbl-b complexed with its small inhibitory peptide, Cblin2016
Author(s)
Ohno A, Ochi A, Maita N, Ueji T, Bando A, Nakao R, Hirasaka K, Abe T, Teshima-Kondo S, Nemoto H, Okumura Y, Higashibata A, Yano S, Tochio H, Nikawa T
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Journal Title
Arch Biochem Biophys
Volume: 594
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] N-Myristoylated ubiquitin ligase Cbl-b inhibitor prevents on glucocorticoid-induced atrophy in mouse skeletal muscle.2015
Author(s)
Ochi A, Abe T, Nakao R, Yamamoto Y, Kitahata K, Takagi M, Hirasaka K, Ohno A, Teshima-Kondo S, Taesik G, Choi I, Kawamura T, Nemoto H, Mukai R, Terao J, Nikawa T.
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Journal Title
Arch Biochem Biophys
Volume: 570
Pages: 23-31
DOI
Related Report
Peer Reviewed
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