Identification of lncRNAs specific for aging of adipose tissue and recovery from diabetic conditions by regulation of their expression
| Project/Area Number |
15K16540
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | Hamamatsu University School of Medicine (2016-2017)
National Center for Geriatrics and Gerontology (2015) |
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Principal Investigator |
Iwashita Yuji 浜松医科大学, 医学部, 助教 (70730406)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 分子生物学 / 老化 / 脂肪 / マイクロアレイ / lncRNA / siRNA / shRNA |
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| Outline of Final Research Achievements |
To reveal how the long non-coding RNAs (lncRNAs) that are not translated into proteins are associated with tissue dysfunction and pathogenesis during aging, difference of gene expression in visceral and subcutaneous adipose tissue were compared between young, middle-aged and old mouse by microarray analysis. In addition to proteins related to cellular senescence and chronic inflammation, novel senescence-associated lncRNAs were differentially expressed in an age-dependent manner. By inhibition of expression of these genes, expression of other marker proteins in aging were altered and reduction of cell numbers were observed. These results suggest that senescence-associated lncRNAs are required for maintenance of senescent cells.
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Report
(4 results)
Research Products
(8 results)
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[Presentation] Identification of gastric cancer-related DNA adducts by DNA adductome analysis2017
Author(s)
Iwashita Y, Ohnishi I, Kurabe N, Yamashita T, Ohtsuka S, Suzuki M, Kahyo T, Iwaizumi M, Matsushima Y, Totsuka Y, Ochiai H, Matsumoto K, Suzuki S, Mori H, Ozawa T, Tanioka F, Sugimura H,
Organizer
48th Annual Meeting of the Environmental Mutagenesis and Genomics Society
Related Report
Int'l Joint Research
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[Presentation] Identification of DNA Adducts in Human Gastric Mucosa by DNA Adductome Analysis2017
Author(s)
Yuji Iwashita, Ippei Ohnishi, Nobuya Kurabe, Takashi Yamashita, Shunsuke Ohtsuka, Masako Suzuki, Tomoaki Kahyo, Moriya Iwaizumi, Yoshitaka Matsushima, Yukari Totsuka, Hideto Ochiai, Keigo Matsumoto, Shioto Suzuki, Hiroki Mori, Takachika Ozawa, Fumihiko Tanioka and Haruhiko Sugimura
Organizer
THE 12TH INTERNATIONAL CONFERENCE & 5TH ASIAN CONGRESS ON ENVIRONMENTAL MUTAGENS
Related Report
Int'l Joint Research
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